Cooh modified europium nanoparticles eunps

Bovine serum albumin (BSA) and Tween-20 were obtained from Solarbio (Beijing, China). EDC was obtained from TCI (Shanghai, China). COOH-modified europium nanoparticles (EuNPs) were obtained from Thermo (USA). Mouse anti-pig monoclonal IgG antibodies and rabbit anti-goat IgG antibodies were obtained from Beijing Biolab Technology (Beijing, China). Goat anti-rabbit IgG was obtained from Cell Signal Technology (USA). NC membranes (Millipore 135) and Ultra-15 3 kDa centrifugal filters were obtained from Millipore (USA). The sample pads, absorbent paper and plastic backing were obtained from Jinbiao Biotech (Shanghai, China). BCA kits were obtained from Beyotime Biotechnology (Shanghai, China). The ES commercial ELISA kit was obtained from Qiagen (Germany).
XYZ3060 dispenser was obtained from Biodot (USA). A TRF fluorescence quantitative analyzer was obtained from Weice Biotech (Nanjing, China). A UV lamp was obtained from Shenzhen Feike Technology (Shenzhen, China).

COOH-modified europium nanoparticles (EuNPs), were obtained from Thermo Fisher (USA); 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC), N-hydroxysulfosuccinimide (NHS), were obtained from Tokyo Chemical Industry (TCI Shanghai, China); mouse anti-human IgG antibody, goat anti-rabbit IgG antibody, rabbit anti-goat IgG antibody, were obtained from Beijing Biolab Technology (Beijing, China); nitrocellulose filter membrane (CN140), sample pads (SB06, SB08, VL78, VL98 and RB45), absorbent pad, plastic backing, were obtained from Jinbiao Biotech (Shanghai, China); and bovine serum albumin (BSA), Tween-20, sucrose, were obtained from Solarbio (Beijing, China). The Time-resolved fluorescence (TRF) quantitative analyzer was obtained from Weice Biotech (Nanjing, China). The CSTR1 antigen, composed of four repeating units, was synthesized by Sangon Biotech (Shanghai) Co., Ltd. (Shanghai, China) at a concentration of 1 mg/mL.