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Rabbit anti rfp

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Rabbit anti-RFP is a primary antibody that specifically binds to the red fluorescent protein (RFP) reporter molecule. It is suitable for the detection and quantification of RFP-tagged proteins in various applications, such as Western blotting, immunohistochemistry, and flow cytometry.

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Lab products found in correlation

Mouse anti gfp, by Thermo Fisher Scientific (5 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (3 mentions) Chicken anti gfp, by Thermo Fisher Scientific (2 mentions) 780 upright confocal microscope, by Zeiss (2 mentions) Donkey anti chicken alexa 647, by Thermo Fisher Scientific (2 mentions) Rabbit anti nestin, by Merck Group (2 mentions) Chicken anti map2, by Abcam (2 mentions) Rabbit anti gaba, by Merck Group (2 mentions) Goat anti sox2, by Santa Cruz Biotechnology (2 mentions) Mouse anti syn1, by Merck Group (2 mentions) Lsm 880 confocal microscope, by Zeiss (2 mentions) Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 goat anti chicken, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488 goat anti rat, by Thermo Fisher Scientific (2 mentions) Rat anti elav 7e8a10, by Developmental Studies Hybridoma Bank (2 mentions) Vectashield mounting medium, by Vector Laboratories (2 mentions) Axiovert 200, by Zeiss (1 mentions) Coolsnap hq, by Teledyne (1 mentions) Alexa conjugated secondary antibodies, by Jackson ImmunoResearch (1 mentions) Anti brp, by Thermo Fisher Scientific (1 mentions)

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Anti-RFP (13 citations)

15 protocols using rabbit anti rfp

1

Immunostaining of Drosophila Larval Neurons

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Third-instar larvae were dissected, fixed in Bouin’s fixative or 4% PFA in PBS, and immunostained as previously described (Eaton et al., 2002 (link); Harris et al., 2015 (link)). Dissected third instar larvae were fixed with PFA (4%) and incubated overnight at 4 C with primary antibodies (mouse anti Flag 1:50; rabbit anti-RFP 1:100; rabbit anti-Dlg, 1:1000; anti-Syt1 1:1000, anti-Brp 1:100, Life Technologies). Alexa-conjugated secondary antibodies and goat anti-HRP were used (Jackson Laboratories 1:500). Images were acquired with either a Zeiss LSM700 confocal microscope equipped with Zen software using a 63X 1.6 NA oil immersion objective or an upright epifluorescence deconvolution confocal microscope (Axiovert 200, Zeiss) equipped with a 100X objective (N.A. 1.4), cooled CCD camera (CoolSnap HQ, Roper Scientific). Slidebook 5.0 (3I, Intelligent Imaging) was used for capturing, deconvolving and analyzing images. Structured illumination microscopy (Nikon LSM 710 equipped with 63X objective and Andor Ixon EMCCD camera) was used to perform Brp-GFP and MCTP-Flag colocalization experiments. Bouton numbers and Brp numbers and densities were quantified as described previously (Harris et al., 2015 (link)).
Genç Ö., Dickman D.K., Ma W., Tong A., Fetter R.D, & Davis G.W. (2017). MCTP is an ER-resident calcium sensor that stabilizes synaptic transmission and homeostatic plasticity. eLife, 6, e22904.
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2

Larval Fillet Immunostaining Protocol

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Earlier third instar larval fillets were stained with the following primary antibodies: chicken anti-GFP (1:5000, Abcam), mouse anti-GFP (1:5000, Life Technologies), and rabbit anti-RFP (1:5000, Life Technologies). The following secondary antibodies were used: donkey anti-chicken Alexa Fluro® 488(1:500, Jackson ImmunoResearch Inc), donkey anti-mouse Alexa Fluro® 488 (1:500, Jackson ImmunoResearch Inc) and donkey anti-rabbit Rhodamine RX (1:500, Jackson ImmunoResearch Inc).
Li R., Liang Y., Zheng S., He Q, & Yang L. (2019). The atypical cadherin flamingo determines the competence of neurons for activity-dependent fine-scale topography. Molecular Brain, 12, 109.
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3

Imaging of Drosophila Imaginal Discs

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The following antibodies used were: rabbit anti-aPKC (1:500; Santa Cruz); rat anti-crb (1:500; gift from E.Knust); mouse anti-Dlg (1:500; Hybridoma Bank); rabbit anti-dpERK (1:200; Cell signalling); goat anti-GFP (1:500; Abcam); rabbit anti-RFP (1:300; Life Technologies); Phalloidin TRITC (1:200; Sigma Aldrich); mouse anti-Patched (1:100; Hybridoma Bank); rat anti-Srp (1:500 made in the Casanova lab). The TUNEL assay was performed using the In situ Cell Death Detection Kit (Roche). Cy2, Cy3 and Cy5-conjugated secondary antibodies were from Molecular Probes and were used at 1:200 dilutions, and discs were mounted in Vectashield containing DAPI. Confocal images were acquired with a Leica SP5. Images were analysed with Fiji software [National Institutes of Health (NIH) Bethesda, MD] and assembled into figures using both Fiji and the Adobe Photoshop software.
Campbell K., Lebreton G., Franch-Marro X, & Casanova J. (2018). Differential roles of the Drosophila EMT-inducing transcription factors Snail and Serpent in driving primary tumour growth. PLoS Genetics, 14(2), e1007167.
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4

Neuronal Morphology of NRXN1+/- hiPSC-Neurons

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Unlabeled control hiPSC-NPCs were plated onto 24-well matrigel coated plates with coverslips. Control hiPSC-NPCs were transduced with a scramble_gRNA-eGFP-puro expressing lentivirus while NRXN1+/− hiPSC-NPCs were transduced with scramble_gRNA-tdTomato-puro expressing lentivirus. A small number of control tdTomato labeled and NRXN1+/− eGFP-labeled hiPSC-NPCs were spiked into the unlabeled background of hiPSC-NPCs upon plating. hiPSC-NPCs were then differentiated for 6 weeks into hiPSC-neurons and immunostained using mouse anti-GFP (ThermoFisher), 1:1,000; rabbit anti-RFP (ThermoFisher) 1:1,000; with Alexa donkey anti-mouse-488 and Alexa donkey anti-rabbit-568 secondary antibodies as described above. Individual neurons were imaged at 20x on the Zeiss 780 upright confocal microscope, followed by tracing and analysis using ImageJ Simple Neurite Tracer Plugin. One-way ANOVA with Dunnett’s test was used to test the impact of genotype on neuronal morphology. As only the eGFP 3’-NRXN1+/− were imaged on the same coverslip as tdTomato labeled controls, we assess the impact of coverslip to coverslip variation between these lines using a two-way ANOVA.
Flaherty E., Zhu S., Barretto N., Cheng E., Deans M.P., Fernando M., Schrode N., Francoeur N., Antoine A., Alganem K., Halpern M., Deikus G., Shah H., Fitzgerald M., Ladran I., Gochman P., Rapoport J., Tsankova N., Mccullumsmith R., Hoffman G.E., Sebra R., Fang G, & Brennand K.J. (2019). Neuronal impact of patient-specific aberrant NRXN1α splicing. Nature genetics, 51(12), 1679-1690.
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5

Immunostaining of Brain Tissue and Cells

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Immunostaining of brain sections and dissociated cells were performed as described previously.66 (link), 67 (link) The following primary antibodies were used: Chicken anti-GFP (Thermo Fisher Scientific Inc.), rabbit anti-GFP (Thermo Fisher Scientific Inc.), rabbit anti-RFP (Thermo Fisher Scientific Inc.), mouse anti-MAP2 (BioLegend, San Diego, CA, USA), guinea pig anti-vGlut (EMD Millipore, Billerica, MA, USA), rabbit anti-vGAT (PhosphoSolutions, Aurora, CO, USA), mouse anti-GAD6 (DSHB) and mouse anti-Sig-1 R (Santa Cruz Biotechnology, Inc., Dallas, TX, USA)). Appropriate secondary antibodies conjugated with Alexa Fluor dyes (Thermo Fisher Scientific Inc) were used to detect primary antibodies.
Ka M., Kook Y.H., Liao K., Buch S, & Kim W.Y. (2016). Transactivation of TrkB by Sigma-1 receptor mediates cocaine-induced changes in dendritic spine density and morphology in hippocampal and cortical neurons. Cell Death & Disease, 7(10), e2414-.
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6

Immunocytochemistry of hiPSC-derived Neurons

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hiPSC-NPCs, hiPSC-neurons and NGN2-neurons were fixed in 4% paraformaldehyde in PBS at room temperature for 10 min. hiPSC-NPCs were permeabilized at room temperature for 15 min in 1.0% Trition in PBS. All cells were blocked in 5% donkey serum with 0.1% Triton at room temperature for 30 min. Primary antibodies, including rabbit anti-NESTIN (Millipore), 1:500; goat anti-SOX2 (Santa Cruz), 1:500; chicken anti-MAP2 (Abcam), 1:500–1:5,000; mouse anti-SYN1 (Millipore), 1:500; mouse anti-GFP (ThermoFisher), 1:1,000; rabbit anti-RFP (ThermoFisher), 1:1,000, rabbit anti-GABA (Sigma) 1:500; were incubated overnight at 4 °C. Secondary antibodies including Alexa donkey anti-rabbit 488, 568, 647 (Invitrogen), Alexa donkey anti-mouse 488, 568, 647 (Invitrogen) and Alexa donkey anti-chicken 647 (Invitrogen) were used at 1:500–1:1,000. DAPI (0.5 μg/mL) was used to visualize nuclei. Coverslips were mounted with Vectashield and imaged using the Zeiss upright 780 confocal microscope.
Flaherty E., Zhu S., Barretto N., Cheng E., Deans M.P., Fernando M., Schrode N., Francoeur N., Antoine A., Alganem K., Halpern M., Deikus G., Shah H., Fitzgerald M., Ladran I., Gochman P., Rapoport J., Tsankova N., Mccullumsmith R., Hoffman G.E., Sebra R., Fang G, & Brennand K.J. (2019). Neuronal impact of patient-specific aberrant NRXN1α splicing. Nature genetics, 51(12), 1679-1690.
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7

Immunocytochemistry of hiPSC-derived Neurons

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hiPSC-NPCs, hiPSC-neurons and NGN2-neurons were fixed in 4% paraformaldehyde in PBS at room temperature for 10 min. hiPSC-NPCs were permeabilized at room temperature for 15 min in 1.0% Trition in PBS. All cells were blocked in 5% donkey serum with 0.1% Triton at room temperature for 30 min. Primary antibodies, including rabbit anti-NESTIN (Millipore), 1:500; goat anti-SOX2 (Santa Cruz), 1:500; chicken anti-MAP2 (Abcam), 1:500–1:5,000; mouse anti-SYN1 (Millipore), 1:500; mouse anti-GFP (ThermoFisher), 1:1,000; rabbit anti-RFP (ThermoFisher), 1:1,000, rabbit anti-GABA (Sigma) 1:500; were incubated overnight at 4 °C. Secondary antibodies including Alexa donkey anti-rabbit 488, 568, 647 (Invitrogen), Alexa donkey anti-mouse 488, 568, 647 (Invitrogen) and Alexa donkey anti-chicken 647 (Invitrogen) were used at 1:500–1:1,000. DAPI (0.5 μg/mL) was used to visualize nuclei. Coverslips were mounted with Vectashield and imaged using the Zeiss upright 780 confocal microscope.
Flaherty E., Zhu S., Barretto N., Cheng E., Deans M.P., Fernando M., Schrode N., Francoeur N., Antoine A., Alganem K., Halpern M., Deikus G., Shah H., Fitzgerald M., Ladran I., Gochman P., Rapoport J., Tsankova N., Mccullumsmith R., Hoffman G.E., Sebra R., Fang G, & Brennand K.J. (2019). Neuronal impact of patient-specific aberrant NRXN1α splicing. Nature genetics, 51(12), 1679-1690.
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8

Comprehensive Antibody Characterization for Cellular Studies

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Our studies employed the following primary antibodies: Rabbit monoclonal anti-Engrailed (Monoclonal Antibody Facility, Institute of Neuroscience, University of Oregon), mouse polyclonal anti-Prox1 (AngioBio, 11-002P), mouse anti-FLAG M1 (prepared in-house, 1:5,000), rabbit anti-FLAG M2 (Sigma, F7425, 1:1,000), rabbit anti-GFP (which also detects YFP) (Thermo Fisher Scientific A11122, 1:5,000), mouse anti-PKA-C (BD Biosciences, 610980, 1:5,000), mouse anti-PKA-R (BD Biosciences, 610609, 1:500), mouse anti Arl13b (Antibodies, 75–287, 1:1,000), rabbit anti-RFP (Thermo Fisher Scientific, R10367, 1:1,000), rabbit anti-CREB (Cell Signaling Technology, 9197S, 1:1,000), and rabbit anti phospho-CREB (Cell Signaling Technology, 9198S, 1:1,000). For chemiluminescent western blots, HRP-conjugated anti-mouse and anti-rabbit secondary antibodies were obtained from Promega and used at 1:20,000. For infrared western blots, IR680- and IR800-conjguated secondary antibodies (LiCor) were used at 1:20,000. For immunofluorescence, AlexaFluor-conjugated secondary antibodies were obtained from Thermo Fisher Scientific and used at 1:1,000. M1 FLAG affinity resin and M1 FLAG-Alexa 647 conjugates were prepared in house.
Arveseth C.D., Happ J.T., Hedeen D.S., Zhu J.F., Capener J.L., Klatt Shaw D., Deshpande I., Liang J., Xu J., Stubben S.L., Nelson I.B., Walker M.F., Kawakami K., Inoue A., Krogan N.J., Grunwald D.J., Hüttenhain R., Manglik A, & Myers B.R. (2021). Smoothened transduces Hedgehog signals via activity-dependent sequestration of PKA catalytic subunits. PLoS Biology, 19(4), e3001191.
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9

Neuronal Morphology of NRXN1+/- hiPSC-Neurons

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Unlabeled control hiPSC-NPCs were plated onto 24-well matrigel coated plates with coverslips. Control hiPSC-NPCs were transduced with a scramble_gRNA-eGFP-puro expressing lentivirus while NRXN1+/− hiPSC-NPCs were transduced with scramble_gRNA-tdTomato-puro expressing lentivirus. A small number of control tdTomato labeled and NRXN1+/− eGFP-labeled hiPSC-NPCs were spiked into the unlabeled background of hiPSC-NPCs upon plating. hiPSC-NPCs were then differentiated for 6 weeks into hiPSC-neurons and immunostained using mouse anti-GFP (ThermoFisher), 1:1,000; rabbit anti-RFP (ThermoFisher) 1:1,000; with Alexa donkey anti-mouse-488 and Alexa donkey anti-rabbit-568 secondary antibodies as described above. Individual neurons were imaged at 20x on the Zeiss 780 upright confocal microscope, followed by tracing and analysis using ImageJ Simple Neurite Tracer Plugin. One-way ANOVA with Dunnett’s test was used to test the impact of genotype on neuronal morphology. As only the eGFP 3’-NRXN1+/− were imaged on the same coverslip as tdTomato labeled controls, we assess the impact of coverslip to coverslip variation between these lines using a two-way ANOVA.
Flaherty E., Zhu S., Barretto N., Cheng E., Deans M.P., Fernando M., Schrode N., Francoeur N., Antoine A., Alganem K., Halpern M., Deikus G., Shah H., Fitzgerald M., Ladran I., Gochman P., Rapoport J., Tsankova N., Mccullumsmith R., Hoffman G.E., Sebra R., Fang G, & Brennand K.J. (2019). Neuronal impact of patient-specific aberrant NRXN1α splicing. Nature genetics, 51(12), 1679-1690.
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10

Immunostaining Protocols for Drosophila Eye Imaginal Discs

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Wandering 3rd instar larvae were collected from respective genetic crosses and eye imaginal discs were processed for immunostaining as previously described (Sudhakaran et al. 2014 (link)). Each immunostaining experiment was reproduced in at least three independent biological replicates: for each replicate, discs dissected from multiple larvae were individually examined.
Primary antibodies used were: rabbit anti-RFP (Invitrogen R103367,) at 1:1000, rabbit-anti-Rasputin (Aguilera-Gomez et al. 2017 (link)) 1:1000, rabbit-anti-Caprin (Papoulas et al. 2010 (link)) at 1:1000, rat-anti-Elav-7E8A10 (Developmental Studies Hybridoma Bank) 1:300 and chicken-anti-Ataxin-2 (Bakthavachalu et al. 2018 (link)) at 1:500. Secondary antibodies were used at 1:1000 dilution: Alexa Fluor®488 goat anti-Chicken (Invitrogen A11039), Alexa Fluor®488 goat anti-Rabbit (Invitrogen A11078), Alexa Fluor®488 goat anti-Rat (Invitrogen A11006), and Fluor®555 goat anti-Rabbit (Invitrogen A21428).
Prepared eye-imaginal discs were mounted in Vectashield Mounting Medium (Vector Labs, H-1000) on microscope slides and imaged on a Zeiss LSM880 confocal microscope.
Huelsmeier J., Walker E., Bakthavachalu B, & Ramaswami M. (2021). A C-terminal ataxin-2 disordered region promotes Huntingtin protein aggregation and neurodegeneration in Drosophila models of Huntington’s disease. G3: Genes|Genomes|Genetics, 11(12), jkab355.
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