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Bat 10 thermometer

Manufactured by Physitemp
Sourced in United States

The Bat-10 thermometer is a compact and versatile digital thermometer designed for laboratory and scientific applications. It features a fast-response thermistor probe and a large, easy-to-read LCD display. The Bat-10 thermometer can measure temperatures ranging from -50°C to 150°C with an accuracy of ±0.1°C.

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9 protocols using bat 10 thermometer

1

Rectal Temperature Monitoring

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Rectal temperature was monitored with a BAT-10 Thermometer (Physitemp Instruments, Clifton, NJ).
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2

Measuring Animal Body Temperature

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Due to the presence of Zn in the fumes and the possible development of metal fume fever, animal body temperatures were measured before and after each exposure using a Bat-10 thermometer coupled to a RET-2 rat rectal probe (Physitemp Inc, Clifton, NJ).
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3

Temperature Regulation in Mice

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WT and N1+/− mice (n = 5, each) were housed in cages at 4 °C for 8 h, and rectal temperature was recorded with a Bat-10 thermometer coupled to a RET-3 mouse rectal probe (Physitemp, Clifton, NJ) lubricated with mineral oil at a frequency of one data point per 1 h12 (link). The measurement was conducted at 8 weeks of age.
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4

Cold Exposure Body Temperature Measurement

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Six mice of each genotype, aged 2.5 months, were placed at 4°C. Five hours after food removal, body temperature was measured using a rectal thermometer (BAT-10 Thermometer; Physitemp Instruments, Inc., Clifton, NJ).
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5

Metabolic Profiling of Dietary Interventions

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Wild-type C57BL6/J mice at the age of 5–6 weeks were purchased from The Jackson Laboratory (Bar Harbor, ME). All studies were approved by and performed in compliance with the guidelines of the Yeshiva University Institutional Animal Care and Use Committee. The CD and HFD were ground into fine powder and mixed with pure MGF (CTMedChem, Bronx, NY). The mixtures were repelleted and administered to individually housed mice. Mice were fed ad libitum with the CD or HFD, with or without MGF, and water.
Fat composition and fat distribution were determined by MRI. Lean and fat mass was determined as described (17 (link)). Metabolic measurements (Vo2), respiratory quotient (RQ), energy expenditure (EE), and locomotor activity were obtained continuously using a CLAMS (Columbus Instruments) open-circuit indirect calorimetry system (18 (link)). Animals were acclimatized for 2 days, and data were subsequently collected over a 5-day period. Body temperature was measured using a BAT-10 thermometer (Physitemp, Clifton, NJ). Hyperinsulinemic-euglycemic clamps were performed as described (19 (link)). An intraperitoneal glucose tolerance test (IGTT) was performed on conscious mice after 12-h fasting and an intraperitoneal injection with 20% glucose in saline (2 g/kg total body weight).
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6

Metabolic Monitoring in Mice

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Rectal body core temperature was measured using a probe attached to a digital thermometer (BAT-10 thermometer, Physitemp, Clifton, NJ, USA). An 8-channel PromethION etabolic system (Sable Systems International, Las Vegas, Nevada, USA) was used to monitor energy expenditure and respiration in mice. Mice were housed at 24 °C or 4 °C in the PromethION metabolic cages (1 mouse per cage) placed in a temperature-controlled cabinet. The basic metabolic indicators, including diet consumption, O2 consumption and CO2 production, were monitored in real-time according to the manufacturer's instructions.
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7

Ambient Temperature Effects on METH-Induced Thermoregulation

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The ambient room temperature during METH “binge” treatment for Experiment-1 (cold ambient temperature) was 14 ± 1 °C, and for Experiment-2 (hot ambient temperature) was 29 ± 1°C. Mice were placed in a 14 °C or 29 °C environment 1 hour prior to METH exposure and remained there until 3 hours after the last dose of METH.
Core body (rectal) temperature was measured using a Bat-10 thermometer equipped with a RET-3 mouse rectal probe (Physitemp, Inc., Clifton, NJ, USA), as previously described (Raineri et al., 2012 (link)). Core temperatures were recorded immediately prior to the initiation of the experiments (baseline readings), 1 hour after every dose of METH and up to 3 hours after the last dose of METH. Previous results from other laboratories indicate that core body temperatures determined at 1 hour post-injection of METH are representative of the peak changes in temperature (Bowyer et al., 2001 (link)). An additional temperature reading was taken (16 hours after last METH injection) after animals had returned to the animal facility (standard temperature).
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8

Multimodal Temperature Monitoring

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Core temperature was measured using a RET-4 thermocouple sensor, Type T rectal probe (Physitemp Instruments), and BAT10 thermometer (Physitemp Instruments). Whole-body surface temperature was measured using an infrared thermal camera (T650sc, FLIR Systems). Images were taken at the specified time points.
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9

Cold Exposure Rectal Temperature

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WT and N1+/-mice (n = 5, each) were housed in cages at 4 °C for 8 hrs, and rectal temperature was recorded with a Bat-10 thermometer coupled to a RET-3 mouse rectal probe (Physitemp, Clifton, NJ) lubricated with mineral oil at a frequency of one data point per 1 hr 13 (link) . The measurement was conducted at 8 weeks of age.
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