Lsm 700 system

Manufactured by Zeiss

Sourced in Germany

The LSM 700 system is a confocal laser scanning microscope designed for high-resolution imaging of biological samples. It features a compact and modular design, allowing for flexible configuration to meet specific research needs. The system utilizes a laser illumination source and advanced optics to provide detailed, three-dimensional images of samples with high contrast and resolution.

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Lab products found in correlation

4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (4 mentions) Prolong gold antifade reagent, by Thermo Fisher Scientific (3 mentions) Efficient navigation zen , by Zeiss (3 mentions) Zen 2010, by Zeiss (3 mentions) Saponin, by Merck Group (2 mentions) Lsm image browser, by Zeiss (2 mentions) Ab13970, by Abcam (2 mentions) Plan apochromat 40x 1.4 oil objective, by Zeiss (2 mentions) Alexa fluor 594 and alexa fluor 488 conjugated secondary antibodies, by Thermo Fisher Scientific (2 mentions) Anti flag and anti γh2ax antibodies, by Thermo Fisher Scientific (2 mentions) Mouse anti o linked n acetylglucosamine o glcnac hgac85, by Thermo Fisher Scientific (2 mentions) Mouse anti o glcnac transferase c 10, by Santa Cruz Biotechnology (2 mentions) Pe conjugated goat anti rabbit igg, by Santa Cruz Biotechnology (2 mentions) Fluorsave, by Merck Group (2 mentions) Imagej, by Zeiss (2 mentions) Cell observer, by Zeiss (2 mentions) Arginase 1 arg 1, by Santa Cruz Biotechnology (2 mentions) Prolong gold antifade, by Thermo Fisher Scientific (2 mentions) Axiovision 4, by Zeiss (2 mentions) Plan apochromat, by Zeiss (1 mentions)

Close spelling variants of this product

LSM 700 (4063 citations) LSM 700 confocal microscope system (34 citations) LSM 700 confocal system (32 citations) LSM 700 META confocal system (9 citations) Zeiss LSM 700 laser confocal microscopy system (7 citations) LSM 700 confocal imaging system (6 citations) LSM 700 laser scanning confocal system (5 citations) Laser Scanning System LSM 700 (4 citations) LSM 700 stage imaging system (3 citations) LSM 700 microscope system (3 citations)

48 protocols using lsm 700 system

Optimized Confocal Imaging Protocols

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Immunohistochemistry microscopy of cryosections: Confocal laser scanning microscopy was performed using a LSM-700 system (Zeiss) with a Plan-Apochromat 20x NA 0.8, an EC Plan-Neofluar 40x NA1.30 Oil DIC Plan-Apochromat, or a Plan-Apochromat 63x NA 1.40 oil DIC. Z-stacks were selected to cover the entire section and taken in software (ZEN) suggested optimal size steps.
Immunohistochemistry microscopy of fixed organotypical slice cultures after ex vivo electroporation: Confocal laser scanning microscopy was performed using a LSM-700 system (Zeiss) with a Plan-Apochromat 20x NA 0.8 objective or using a SP8 system (Leica) with a HCX PL FLUOTAR L 20x objective. Z-stacks were selected to cover the entire section and taken in software (ZEN) suggested optimal size steps.

Will L., Portegies S., van Schelt J., van Luyk M., Jaarsma D, & Hoogenraad C.C. (2019). Dynein activating adaptor BICD2 controls radial migration of upper-layer cortical neurons in vivo. Acta Neuropathologica Communications, 7, 162.

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Fluorescence Microscopy Imaging Protocols

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TIRF microscopy imaging was performed on a custom automated Nikon inverted fluorescence microscope using the 100×/1.45 numerical aperture (NA) objective. TIRF illumination was set up and aligned according to the manufacturer’s instructions, as previously described (72 (link)). Confocal microscopy was performed on a Zeiss LSM 700 system (Carl Zeiss).

Azoulay-Alfaguter I., Strazza M., Peled M., Novak H.K., Muller J., Dustin M.L, & Mor A. (2017). The tyrosine phosphatase SHP-1 promotes T cell adhesion by activating the adaptor protein CrkII in the immunological synapse. Science signaling, 10(491), eaal2880.

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Confocal Imaging of Lymphatic Vessels

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Confocal images of lymphatic vessels of the ear skin, the popliteal lymphatic vessels or mesenteric lymphatic vessels were acquired using a Zeiss LSM 700 system (Carl Zeiss) with a 20×/0.8 objective or a Leica SP8 laser confocal microscope system (Leica Microsystems) with either of 25×/1.0, 25×/0.95, 20×/0.75 or 10×/0.3 objectives. The images represent maximum intensity projections of z stacks that, in the case of overviews, were stitched from multiple tile scan images, either manually using Adobe Photoshop (Adobe) or automatically by the Leica LAF software. Images were processed with FIJI (Schindelin et al., 2012 (link)) or Adobe Photoshop software (Adobe). Intensity Adjustments in Fig. 5E′ were applied specifically to the vessel area (dashed line) in order to enhance the visibility of perivascular SMCs. Tiled epi-fluorescence images showing the entire capillary network were acquired using an Axio Observer Z1 system (Carl Zeiss) with a 5×/0.13 objective and images were automatically aligned by Zen blue 2012 software (Carl Zeiss).

Wang Y., Jin Y., Mäe M.A., Zhang Y., Ortsäter H., Betsholtz C., Mäkinen T, & Jakobsson L. (2017). Smooth muscle cell recruitment to lymphatic vessels requires PDGFB and impacts vessel size but not identity. Development (Cambridge, England), 144(19), 3590-3601.

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Immunofluorescence Staining of DCs

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Bone marrow-derived DCs were plated on glass coverslips and fixed by incubation for 15 min on ice in 3.7% w/v paraformaldehyde followed by quenching for 10 min with 50 mM NH₄Cl in PBS. The cells were incubated for 1 h with specific primary antibodies in permeabilization buffer (PBS with 1 mg/mL BSA and 0.05% w/v saponin (Sigma-Aldrich)), washed twice, and incubated for another hour with the fluorescently labelled secondary antibody in permeabilization buffer. Lastly, the cells were mounted on slides in Prolong Gold antifade reagent in the presence of DAPI (Invitrogen Carlsbad, CA). Confocal microscopy was performed with a Zeiss LSM 700 system (Carl Zeiss) and a 63x NA1.4 objective. Images were processed with the Zeiss LSM Image Browser (Carl Zeiss) and ImageJ software (version 1.43).

Belabed M., Mauvais F.X., Maschalidi S., Kurowska M., Goudin N., Huang J.D., Fischer A., de Saint Basile G., van Endert P., Sepulveda F.E, & Ménasché G. (2020). Kinesin-1 regulates antigen cross-presentation through the scission of tubulations from early endosomes in dendritic cells. Nature Communications, 11, 1817.

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Biofilm Formation on Glass Coverslips

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Bacteria were cultured as described for the bead biofilm system, but instead of inoculating into bead-containing tubes, cultures were diluted into 1 ml M9 citrate in uncoated polystyrene 24-well plates. Each well contained one sterile 10-mm-diameter glass coverslip. Biofilms were grown in 37°C with 200 rpm shaking for 24 h. The glass coverslips were removed with forceps, gently washed three times in 10 ml PBS, and immediately mounted on a glass slide for confocal microscopy with a Zeiss LSM 700 system. Images were acquired and analyzed using Zeiss’s Zen software.

Irie Y., Roberts A.E., Kragh K.N., Gordon V.D., Hutchison J., Allen R.J., Melaugh G., Bjarnsholt T., West S.A, & Diggle S.P. (2017). The Pseudomonas aeruginosa PSL Polysaccharide Is a Social but Noncheatable Trait in Biofilms. mBio, 8(3), e00374-17.

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TRAIL-Induced Apoptosis Quantification

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GC cells were treated with the different concentrations of TRAIL for 24 h. Then a TUNEL Detection Kit (Roche, Basel, Switzerland) was used to determine cell apoptosis, according to the manufacturer’s protocol. Cell confocal images were acquired with a Zeiss LSM700 system (Carl Zeiss, Jena, Germany). Percentages of apoptotic cells are shown from at least 1000 cells, and data were obtained from three independent experiments.

Wang Q., Chen Q., Zhu L., Chen M., Xu W., Panday S., Wang Z., Li A., Røe O.D., Chen R., Wang S., Zhang R, & Zhou J. (2017). JWA regulates TRAIL-induced apoptosis via MARCH8-mediated DR4 ubiquitination in cisplatin-resistant gastric cancer cells. Oncogenesis, 6(7), e353-.

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Detailed Immunostaining and Western Blot Protocol

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The following primary antibodies were used: Chicken anti-GFP (ab13970, 1:1000 (IF); Abcam); Rat anti-Ecadherin (ECCD-2, 1:500 (IF); Thermofisher), and anti-K8 (TROMA-I, 1:200 (IF); DSHB); Rabbit anti-SMA (ab5694, 1:200 (IF); Abcam), anti-ER (E115, 1:200 (IF), 1:500 (WB); Abcam), anti-PR (D8Q2J, 1:600 (IF); Cell Signaling), anti-HER2 (29D8, 1:400 (IF); Cell Signaling), anti-Tsc22d1 (A303–582A, 1:200 (IF), 1:400 (WB); Bethyl), and anti-p53 (CM5, 1:200; Leica); Guinea pig anti-K14 (BP5009, 1:1000 (IF); Origene); Mouse anti-Pten (CPTC-PTEN-1, 1:200 (IF); DSHB), and anti-Cdkn2a (2D9A12, 1:100 (IF); Abcam). Tissue slides were processed for immunostaining as previously described (Beronja et al., 2013 (link); Beronja et al., 2010 (link)), and mounted in ProLong Gold antifade reagent with or without DAPI (Life Technologies). Confocal images were taken on a Zeiss LSM700 system. Images were processed using Zen (Zeiss) and ImageJ software (Schindelin et al., 2012 (link)). Western blot was performed using iBlot system (Invitrogen) and imaged using Odyssey Fc (LI-COR).

Ying Z, & Beronja S. (2020). Embryonic barcoding of equipotent mammary progenitors functionally identifies breast cancer drivers. Cell stem cell, 26(3), 403-419.e4.

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Quantifying Clone Morphology in Skin

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Head skin whole-mounts were imaged on a Zeiss LSM700 system with a Plan-Apochromat 40X/1.4 oil objective. Using ImageJ clone outlines were traced and an ellipse was fitted to each clone. The perimeter and area of the ellipse was measured using ImageJ. Circularity was calculated using the following equation: circularity = 4π(area / perimeter²).

Sandoval M., Ying Z, & Beronja S. (2021). Interplay of opposing fate choices stalls oncogenic growth in murine skin epithelium. eLife, 10, e54618.

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Comprehensive Immunostaining of Tissue Samples

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The following primary antibodies were used: chicken anti-GFP (ab13970, 1:1000; Abcam); mouse anti-BrdU (MoBU-1, 1:100; Invitrogen); rabbit anti-K10 (Poly19054, 1:1,000 BioLegend); rabbit anti-RFP (6000-401-379, 1:1000; Rockland); rat anti-Nidogen (sc-33706, 1:1000; Santa Cruz Biotechnology). Tissues were processed for immunostaining as previously described^4,5 and mounted in ProLong Gold (Invitrogen) with or without 4,6-diamidino-2-phenylindole (DAPI; Life Technologies). Confocal images were taken on a Zeiss LSM700 system using a Plan-Apochromat 40X/1.4 oil objective. Image processing was done using Zeiss Zen and ImageJ software.

Sandoval M., Ying Z, & Beronja S. (2021). Interplay of opposing fate choices stalls oncogenic growth in murine skin epithelium. eLife, 10, e54618.

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Immunostaining Protocol for Müller Cells

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Fresh frozen sections were permeabilized and blocked in a humidified chamber for 1 h at room temperature with 3% horse serum in PBS with 0.3% Triton X-100. The slides were exposed for 2 h to primary antibodies diluted in the same solution. Anti-Sox-9 (1:100, Rb Cell Signaling 82630) was used to mark Müller cells. Slides were washed three times with PBS. Secondary antisera (Alexa-Fluor 488 and 647) diluted 1:10,000 in the serum solution were added for 1 h at room temperature followed by two washes with PBS. Labeling for 30 min at room temperature (Apply Click-iT Invitrogen Alexa Fluor 555 C10338) was followed by a wash with PBS. After incubation with DAPI (1:10,000 in PBS) for 5 min and the application of mounting solution, sections were imaged by confocal microscopy with a Zeiss LSM 700 system with a ×20/0.8 NA objective lens with a pinhole size set at the first Airy disc.

Kastan N., Gnedeva K., Alisch T., Petelski A.A., Huggins D.J., Chiaravalli J., Aharanov A., Shakked A., Tzahor E., Nagiel A., Segil N, & Hudspeth A.J. (2021). Small-molecule inhibition of Lats kinases may promote Yap-dependent proliferation in postmitotic mammalian tissues. Nature Communications, 12, 3100.

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