A CCK8 assay was employed to test the viability of Jar cells transfected with Flag-HLA-F/Flag-NC or siRNA-HLA-F/siRNA-NC. In brief, approximately 5000 Jar cells were seeded into 96-well plates and cultured for 48 h. After reagent treatment, 10 µl of CCK-8 solution was added to each well, and cells were incubated for 2 h. Absorbance at 450 nm was measured using a microplate reader (BioTek® ELx808IU, USA).
Elx808iu
Manufactured by Agilent Technologies
Sourced in United States
The ELx808IU is a multi-function microplate reader manufactured by Agilent Technologies. It is designed to perform absorbance, fluorescence, and luminescence measurements on various microplate formats. The device provides reliable and accurate results for a wide range of applications in life science research and clinical diagnostics.
Automatically generated - may contain errors
Lab products found in correlation
Elx50, by Agilent Technologies (1 mentions)
Cell counting kit 8 (cck8), by Dojindo Laboratories (1 mentions)
Tissuelyser 2, by Qiagen (1 mentions)
Alginate, by Merck Group (1 mentions)
Sodium alginate, by Merck Group (1 mentions)
Tail vein, by Merck Group (1 mentions)
Bradford dye reagent, by Takara Bio (1 mentions)
Bovine serum albumin (bsa), by Beyotime (1 mentions)
Wst 1 assay, by Roche (1 mentions)
Testosterone enzyme immunoassay test kit, by Biocheck (1 mentions)
34 protocols using elx808iu
1
Cell Viability Assay for Jar Cells
2
Serum Hormone Concentration Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Serum nesfatin-1, FSH, LH, and T concentrations were measured using commercial ELISA kits according to the manufacturer’s instruction (Wuhan Xinqidi Biological Technology, Wuhan, China). Briefly, 100 μL of standard or sample was added to each well and incubated for 2 hours at 37 °C. The liquid was then removed from each well, and 100 μL of anti-biotin antibodies was added, and the wells were incubated for 1 hour at 37 °C. After washing thrice with washing buffer (200 μL) using an autowasher (ELx 50, BIOTEK, USA), 100 μL of horseradish peroxidase (HRP)-conjugated avidin was added to each well and incubated for 1 hour at 37 °C. The plates were washed five times as mentioned above. Then, 90 μL of TMB substrate was added to each well and incubated for 20 minutes at 37 °C. Finally, 50 μL of stop solution was added to each well. Absorbance was determined at 450 nm using a microplate reader (ELx808IU, Biotek, USA). The correlation coefficients were more than 0.95 for the standard curve of the FSH, LH, and T assays, and the intra- and inter-assay coefficients of variation (CVs) were less than 9% and 11%, respectively. The sensitivity and detection range for the FSH assay were 0.5 IU/L and 0.5–10 IU/L, respectively; the corresponding values for the LH assay were 2 ng/L and 2–40 ng/L, respectively; and those for the T assay were 3 nmol/L and 3–160 nmol/L, respectively.
3
DPPH-based antioxidant assay for E. alba
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Free radical scavenging activity of the E. alba was estimated using DPPH assay with some modifications from Ref. [19 (link)]. The stock solution of DPPH (0.3 mM) was prepared in ethanol, and 10 μL of plant sample or gallic acid as standard was mixed with 190 μL of DPPH in a 96-well plate with subsequent incubation for 30 minutes in dark. The absorbance was measured using microplate reader (Biotek ELx808IU, USA) at 517 nm, and measurements were used to calculate percentage inhibition of the DPPH solution.
4
Lactate Measurement in Cell Cultures
5
Growth Analysis of Bacterial Strains
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Growth analysis was carried out as previously described with minor modifications68 (link). All strains were first grown overnight in LB broth at 37 °C, harvested the following day, diluted to an OD630 of 0.1 in 200 µl of fresh LB, and placed into 96 well plate. Growth curves were carried out at 37 °C shaking and data was recorded with the BioTek ELx808IU plate reader, measurements taken at OD630 every 30 min.
6
Cell Proliferation Assay with PTX
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cell proliferation was measured by Cell Counting Kit-8 (CCK-8, Dojindo Molecular Technologies Inc., Shanghai, China). OV and OV_PTX cells were plated in 96-well plates with a density of 5 × 103 cells per well and incubated for 24 h. PTX was added with increasing concentrations from 0.001–10 μmol/mL to cells, which were then incubated for 48 h. The cells were then incubated with 10 μL of CCK-8 per well for 1 h at 37 °C. Absorbance was measured at a wavelength of 450 nm using a Bio-Tek ELX808IU absorbance microplate reader (Bio-Tek Instruments Inc., USA).
7
Bacterial Growth Curve Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Growth curves were initiated by growing the 1026b wild-type and various mutant strains overnight, then diluting 200× into fresh LB. The 96-well plate was incubated at 37 °C with shaking in the BioTek ELx808IU and measuring the OD600 every 30 min for 30 h. Each growth curve was done in triplicate and average was presented with the standard error of the mean (s.e.m.).
8
Antibacterial Activity of C. cajan Seed Extracts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The antibacterial activity against the multidrug-resistant S. aureus ATCC BAA-44, S. aureus ATCC 25923, S. aureus ATCC 6538, and S. aureus coagulase (−) of the C. cajan seed extracts was evaluated by agar-well diffusion assay. Overnight grown bacterial culture was inoculated in 5 mL Mueller–Hinton Broth (MHB) and adjusted to an optical density of 1 × 106 CFU/mL as measured using a microplate reader (ELx808IU/BioTek Instruments, Inc., Winooski, VT, USA). The bacterial suspensions were then mixed with 1% Mueller–Hinton Agar (MHA) and dispensed into sterile Petri dishes. The mixture was allowed to solidify, and wells were made using a sterile borer. The seed extract (20 mg/well), DMSO (negative control) and tetracycline 5 mg/mL (positive control) were dispensed into the wells. The plates were then incubated at 37 °C for 18–24 h. The zones of inhibition were measured in millimeters (mm) using a caliper.
9
Cell Viability Assay using MTT
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were plated in 96-well plates and incubated overnight, and then cells were exposed to test compounds for 24 h. Subsequently, cells were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Briefly, 10 μL of MTT was added to each well and incubated for 4 h. The media was removed, and 150 μL of DMSO was added to each well to dissolve the crystal formazan dye. The absorbance was measured at 570 nm on an enzyme-linked immunosorbent assay reader (ELX808IU, Bio-Tek).
10
DNA Synthesis Inhibition Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Analysis of DNA synthesis was measured by a Cell Proliferation ELISA, BrdU (colorimetric) kit (Roche, Mannheim, Germany). This immunostaining procedure is based on measuring the incorporation of bromodeoxyuridine (BrdU) into nuclear DNA in place of thymidine during the S-phase of the cell cycle using specific anti-BrdU antibodies [25 (link)]. Hence, such method provides a colorimetric measurement for DNA synthesis inhibition ratio of the carcinogenic cells. Firstly cells were seeded into 96 well flat-bottomed microtiter plates at a density of 2 × 103. The tumor cells were cultured in the presence of various doses of compounds 2 , 3 , 5 , 9 , 10 and cisplatin. Microtiter plates were incubated at 37 °C in a 5% CO2/95% air humidified atmosphere for 24 h. Cells were labeled with 10 µL BrdU solution for 2 h and then fixed. Anti-BrdU-POD (100 µL) was added and incubated for 90 min. Finally, wells were washed with BPS and cells were incubated with substrate. Absorbance of the samples was measured with an ELX808-IU Bio-Tek apparatus at 492 nm. All experiments were repeated two times. For each compound dose, dublicate wells were used.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!