Male newborn rats were obtained in our laboratory by mating females with males of the same colony of specific pathogen-free rats of the Sprague-Dawley strain purchased from Charles River Laboratories Japan, Inc. (Kanagawa, Japan). One foster mother with 8 male newborns were housed together in plastic Econ cages (W 340 mm × D 450 mm × H 185 mm) with bedding (White flakes: Charles River Laboratories Japan, Inc.) in an environmentally controlled animal room (temperature, 23 ± 3ºC; relative humidity, 50 ± 20%; air ventilation rate, 10–15 times per hour; lighting, 12 h/12 h light/dark cycle) and fed an irradiation-sterilized pelleted diet (NMF, Oriental Yeast Co., Ltd., Tokyo, Japan) and tap water ad libitum. Finally, a total of fifty 6-day-old male rats were subjected to the experiment. The protocol of this study was reviewed and approved by the Animal Care and Use Committee of BoZo Research Center.
White flakes
Manufactured by Charles River Laboratories
Sourced in Japan
White flakes are a powder-like substance used in various laboratory applications. The product's core function is to serve as a material component in experimental processes, providing a consistent and uniform physical format for study and analysis. No further details or interpretations about the intended use of this product are provided.
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3 protocols using white flakes
1
Sprague-Dawley Rat Newborn Model
2
Mouse Housing and Feeding Study
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Male Crj: CD-1 (ICR) mice (Charles River Laboratories Japan, Inc.) were 7 weeks of age at the time of treatment. Animals were housed in aluminum cages with absorbent bedding (White Flakes, Charles River Laboratories Japan, Yokohama, Japan) in a specific pathogen free facility with a 12-h light/12-h dark cycle. Mice were provided cobalt-60 irradiated solid feed (CE-2, CLEA Japan, Inc., Tokyo, Japan) and water ad libitum.
3
Sprague Dawley Rat Housing and Acclimation
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Four-week-old male and female Sprague Dawley (SD) rats were obtained from Charles River Laboratories Japan, Inc. (Kanagawa, Japan). They were quarantined and acclimatized for 9 days and 3 weeks after receipt for the first and second experiments (Experiment 1 and Experiment 2), respectively. The rats were housed individually in cages with a bedding (White flakes, Charles River Laboratories Japan, Inc.) under controlled temperature (23°C ± 3°C) and humidity (50% ± 20%) conditions and a 12-hr light/dark cycle. Basal diet (MF, Oriental Yeast Co., Ltd., Tokyo, Japan) and drinking water were provided ad libitum. The maintenance and experimental protocols conformed to the Guide for the Care and Use of Laboratory Animals of Taisho Pharmaceutical Co., Ltd.
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