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Milliplex map magnetic bead kit

Manufactured by Merck Group

The Milliplex MAP Magnetic Bead Kit is a multiplex assay designed for the quantitative detection of multiple analytes in a single sample. The kit utilizes magnetic beads coated with specific capture antibodies to enable the simultaneous measurement of various targets in a sample. The core function of this product is to provide a platform for the multiplexed analysis of biological samples.

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Lab products found in correlation

3 protocols using milliplex map magnetic bead kit

1

Biomarker Profiling in Biological Samples

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Plasma interleukin (IL)-6, IL-10, chemokine (C-C motif) ligand 2 (CCL2), chemokine (C-X-C motif) ligand 9 (CXCL9), CXCL10, and granulocyte-colony stimulating factor (G-CSF) were measured using a Milliplex map magnetic bead kit (EMD Millipore). Indoleamine 2,3-dioxygenase (IDO) activity was evaluated by measuring kynurenine and tryptophan levels and arginase activities by measuring ornithine and arginine concentrations by liquid chromatography coupled with tandem mass spectrometry (LC MS-MS).
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2

Aqueous Humor Cytokine Profiling

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AC taps were performed pre-operatively and at each post-operative time point using aseptic technique with 30G needle to obtain 100 μl aqueous humor from each eye. Aqueous humor samples were stored in − 80 °C before analyses. Cytokine assay was performed using the MILLIPLEX MAP Magnetic Bead Kit (Merck Millipore, Billerica, MA) according to instructions by the manufacturers. Cytokine levels were measured using the Bio-Plex 200 system (Bio-Rad Laboratories, Hercules, CA) and values were normalized to total protein content of each sample.
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3

Inflammatory Biomarker Profiling

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Plasma levels of Myeloperoxidase (MPO), neutrophil gelatinase-associated lipocalin (NGAL) (R&D Systems), S100A8/9, S100A12, and Arginase I (Hycult Biotech) were determined by ELISA. Plasma levels of G-CSF, IL-10, and IL-12 were measured using a Milliplex map magnetic bead kit (EMD Millipore). IDO and arginase activities were respectively evaluated by measuring kynurenine and tryptophan levels by high-performance liquid chromatography, and ornithine and arginine concentrations by ion-exchange chromatography (11, 30) .
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