T0901317 was purchased from Cayman Chemical (Ann Arbor, Michigan, USA). Gefitinib (Iressa) was purchased from AstraZeneca UK Limited (Macclesfield, UK). All drugs were suspended in DMSO and stored at −20°C. Antibodies against MMPs, E-cadherin, and β-actin were obtained from Cell Signaling Technology (Danvers, Massachusetts, USA).
Gefitinib iressa
Manufactured by AstraZeneca
Sourced in United Kingdom
Gefitinib (Iressa) is a small-molecule tyrosine kinase inhibitor. It is a targeted therapy used to treat certain types of non-small cell lung cancer.
Automatically generated - may contain errors
Lab products found in correlation
E cadherin, by Cell Signaling Technology (2 mentions)
β actin, by Cell Signaling Technology (2 mentions)
T0901317, by Cayman Chemical (1 mentions)
Actin ac15, by Merck Group (1 mentions)
Pemetrexed, by Eli Lilly (1 mentions)
P erk1 2 t202 y204, by Cell Signaling Technology (1 mentions)
P met y1234 y1235, by Cell Signaling Technology (1 mentions)
β tubulin, by Cell Signaling Technology (1 mentions)
N cadherin, by Cell Signaling Technology (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
P pi3k, by Cell Signaling Technology (1 mentions)
Erlotinib, by Roche (1 mentions)
P akt ser473, by Abcam (1 mentions)
4 protocols using gefitinib iressa
1
Protein Expression and Antibody Validation
2
Combination Therapy for Cancer Treatment
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T‐QY305 (Beijing Tcmages Pharmaceutical Co., Ltd), gefitinib (IRESSA, AstraZeneca), minocycline (Minocin, Hanhui Pharmaceuticals Co., Ltd), loperamide (Imodium, Xian Janssen pharmaceutical Ltd)
3
Lung Adenocarcinoma Cell Line Cultivation
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The lung adenocarcinoma cell lines CL1-0, CL1-5, and A549 were provided by Dr. Cheng-Wen Wu and Dr. Pan-Chyr Yang [44 (link),45 (link),46 (link)]. Cells were grown in RPMI 1640 medium with 10% heat-inactivated fetal bovine serum and cultured at 37 °C in a humidified atmosphere with 5% CO2. The folate-deficient medium, RPMI 1640 medium without folic acid, was purchased from Gibco (Thermo Fisher Scientific, Waltham, MA, USA). Fetal bovine serum was dialyzed at 4 °C for 16 h against 10 volumes of sterile phosphate-buffered saline and used under the FD condition to minimize the amount of folate from exogenous sources. Pemetrexed from Lilly (Indianapolis, IN, USA). Gefitinib (Iressa) was obtained from AstraZeneca (Macclesfield, Cheshire, UK), and erlotinib (Tarceva) was obtained from Roche (Basel, Switzerland). Antibodies specific for E-cadherin (H-108), vimentin (V9), SLUG (A-7) (Santa Cruz Biotechnology, Dallas, TX, USA), N-cadherin (22200002) (Novus International, Saint Charles, MO, USA), Sox9 (EPR14335) (Epitomics, Wembley, London, UK), and actin (AC-15) (Sigma-Aldrich, St. Louis, MO, USA) were used in Western blot analysis.
4
Investigating Gefitinib and Erlotinib Effects
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Gefitinib (Iressa) was obtained from AstraZeneca (Cheshire, UK) and erlotinib (Tarceva) was purchased from Roche Pharma (Schweiz, UK). The stock solution was prepared in dimethyl sulfoxide (DMSO) at 20 mM and stored at −20°C. MTE (M. tenacissima extract, trade name: Xiao-Ai-Ping injection) (1g crude/ml) was obtained from SanHome Pharmaceutical Co., Ltd (NanJing, China). Our previous HPLC-MS analysis demonstrated that there were at least thirteen C-21 steroids in MTE [29 (link)]. Epidermal growth factor (EGF) and hepatocyte growth factor (HGF) were purchased from Biosource International Inc. (Camarillo, CA). Antibodies against p-PI3K, Akt, mTOR, p-mTOR (Ser2448), EGFR, p-EGFR (Tyr1068), Met, p-Met (Y1234/Y1235), Axl, p-Axl (Tyr702), ERK1/2, p-ERK1/2 (T202/Y204), E-cadherin, N-cadherin, vimentin, β-actin and β-tubulin were purchased from Cell Signaling Technology (Beverly, MA). PI3K and p-Akt (Ser473) were obtained from ABcam (Cambridge, UK).
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