Primary insulin antibody

Manufactured by Abcam

Sourced in United States

The primary insulin antibody is a laboratory reagent used to detect and quantify insulin in biological samples. It functions as a specific binding agent that recognizes and attaches to insulin molecules, allowing for their identification and measurement.

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Lab products found in correlation

Affinipure goat anti mouse igg h l antibody, by Jackson ImmunoResearch (1 mentions) Pannoramic midi and pannoramic viewer, by 3DHISTECH (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Vector Laboratories (1 mentions) 4 6 diamidino 2 phenylindole dapi, by Abcam (1 mentions)

Close spelling variants of this product

Insulin

2 protocols using primary insulin antibody

Insulin Expression in Pancreatic Islets

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The pancreatic isolated islets were fixed and paraffin embedded. Tissue sections
were incubated with primary insulin antibody (Abcam, Cambridge, MA, USA), along
with secondary AffiniPure Goat Anti-Mouse IgG H&L antibody (Jackson
Immunoresearch Laboratories and Molecular Probes, West Grove, PA, USA).
Counterstaining was performed with DAPI (Vector, Burlingame, CA, USA).
Pannoramic MIDI and Pannoramic Viewer (3DHistech, Budapest, Hungary) were used
to scan stained slides and capture images. All the pictures were quantified and
analyzed using Image-Pro Plus software.

Cai X.H., Wang G.Q., Liang R., Wang L., Liu T.L., Zou J.Q., Liu N., Liu Y., Wang S.S, & Shen Z.Y. (2020). CORM-2 Pretreatment Attenuates Inflammation-mediated Islet Dysfunction. Cell Transplantation, 29, 0963689720903691.

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Immunocytochemical Analysis of Insulin Expressing MIN6 Cells

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Wild-type or α-ENaC^−/− MIN6 cells were grown in glass-bottom culture dishes at 37°C with 5% CO₂, after 24 h, cells were fixed (4% paraformaldehyde), permeabilized (5% Triton X-100), blocked (1% bovine serum albumin), and then the cells were incubated with primary insulin antibody (Abcam) at 4°C overnight. Next, the fluorescent mouse IgG (Thermo Fisher) were incubated for 1 h at room temperature and nuclei were stained with 4',6-diamidino-2-phenylindole (DAPI; Abcam). Finally, the cells were observed under the fluorescence microscopy.

Zhang X., Zhao L., Jin R., Li M., Li M.S., Li R, & Liang X. (2021). CRISPR/Cas9-Mediated α-ENaC Knockout in a Murine Pancreatic β-Cell Line. Frontiers in Genetics, 12, 664799.

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