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3 protocols using epicatechin

1

HPLC Analysis of Polyphenol Compounds

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Epicatechin (EC), gallocatechin (GC), catechin gallate (CG), gallocatechin gallate (GCG), epigallocatechin (EGC), Epicatechin gallate (ECG) and catechin (C) were purchased from Chemfaces with purity >98%. Epigallocatechin gallate (EGCG) with guaranteed grade was purchased from Nacalai Tesque. A high-performance liquid chromatography (HPLC) grade acetonitrile was obtained from JT Baker. HepG2 cells (ATCC® HB-8065™) were obtained from Institute of Marine Biotechnology, Universiti Malaysia Terengganu. Alpha-minimum essential media (α-MEM) with SP grade, Penicillin-Streptomycin solution with SP grade and MTT powder with guaranteed reagent (GR) grade were obtained from Nacalai Tesque. Fetal bovine serum (FBS) was obtained from Tico Europe. MEM non-essential amino acids solution, trypsin ethylenediaminetetraacetic acid (EDTA), trypan blue stain, phosphate buffered saline (PBS) tablet, dimethyl sulfoxide (DSMO) with AR grade and BODIPY FL LDL were obtained from Thermo Fisher Scientific. Lipid depleted FBS was obtained from Biowest. Cholesterol powder with purity ≥99%, 25-hydroxylcholesterol with purity ≥98% was purchased from Sigma-Aldrich, while berberine chloride (BC) was purchased from Merck.
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2

Comprehensive Phytochemical Standards Database

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The standards for anthocyanins comprised delphinidin 3-O-glucoside chloride, petunidin 3-O-glucoside chloride, cyanidin chloride (Extrasynthese, Genay, France), and cyanidin 3-O-glucoside chloride (SinoStandards Bio-Tech Co., Ltd., Chengdu, China). The standards for flavan-3-ols consisted of catechin hydrate (Sigma Aldrich Co., St Louis, MO, USA) and epicatechin (Chemfaces, Wuhan, China). The standards for isoflavones comprised daidzein, glycitein, genistein, daidzin, glycitin, genistin (LC Laboratories, Woburn, MA, USA), acetyl daidzin, acetyl glycitin, acetyl genistin (Nacalai Tesque, Kyoto, Japan), malonyl daidzin, malonyl glycitin, and malonyl genistin (GenDEPOT, Katy, TX, USA).
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3

Choline Chloride and Fructose Characterization

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The DES was composed of choline chloride (ChCl) (purity ≥99%) obtained from Gentham Life Science, UK, and D (−)fructose (Fru) (purity ≥99%) purchased from Acros Organics, United States. The solvents, such as methanol (purity ≥99.9%), acetonitrile (purity ≥99.9%) and water (LC-MS grade), were obtained from Honeywell, Germany. The chemical standards were obtained as follows: piceatannol (purity ≥98%), epicatechin (purity ≥98%), procyanidin B1 (purity ≥98%), (−)-epigallocatechin (purity ≥98%) and taxifolin (purity ≥98%) from Chem Faces, China; isorhapontin (purity ≥98%) from Toronto Research Chemicals, Canada; 4-coumaric acid (purity ≥98%) and (+)-catechin (purity ≥98%) from Extrasynthese, France. Ultra-pure water was obtained using a Millipore Milli-Q Plus water treatment system (Millipore Bedford Corp., Bedford, MA).
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