Mouse anti bcl xl

Manufactured by Santa Cruz Biotechnology

Mouse anti-BCL-XL is a primary antibody that specifically binds to the BCL-XL protein in mouse samples. BCL-XL is an anti-apoptotic member of the Bcl-2 family and plays a role in regulating programmed cell death.

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Bcl-xL (224 citations) Anti-Bcl-xL (83 citations) Mouse monoclonal anti-Bcl-XL (2 citations)

2 protocols using mouse anti bcl xl

Western Blot Analysis of Cell Signaling

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Immunoblot analysis was performed
as previously described^{58 (link)−60 (link)} using mouse anti-β-Actin,
mouse anti-p-ERK, rabbit anti-ERK, mouse anti-p-EGFR (Y1045), mouse
anti-p-EGFR (Y1148), mouse anti-p-EGFR (Y1173), mouse anti-CCND1,
mouse anti-CDK4, mouse anti-CCNB1, rabbit anti-c-MYC, mouse anti-BCL2,
mouse anti-BCL-XL, mouse anti-BAD, rabbit anti-CYCS, mouse anti-p-ERK
(44/42), and rabbit anti-ERK antibodies were procured from Santa Cruz
Biotechnology, CA. Mouse anti-CDH1, mouse anti-CDH2, rabbit anti-OCLN,
rabbit anti-pSTAT3, and mouse anti-STAT3 antibodies were obtained
from Abcam, Cambridge, MA. Rabbit anti-p-EGFR (Y992) and rabbit anti-p-CDK2
antibodies were obtained from Cell Signaling. Cell extracts were resolved
on SDS-PAGE and immunoblotted, with the appropriate and respective
antibodies. β-Actin was used as input control for cell lysate.
The sizes of the detected protein bands are shown in kilodaltons on
the left side.

Sebastian A., Pandey V., Mohan C.D., Chia Y.T., Rangappa S., Mathai J., Baburajeev C.P., Paricharak S., Mervin L.H., Bulusu K.C., Fuchs J.E., Bender A., Yamada S., B.a.s.a.p.p.a., Lobie P.E, & Rangappa K.S. (2016). Novel Adamantanyl-Based Thiadiazolyl Pyrazoles Targeting EGFR in Triple-Negative Breast Cancer. ACS Omega, 1(6), 1412-1424.

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Cytotoxicity Evaluation Protocol

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Cis-diammineplatinum dichloride (CDDP), doxorubicin hydrochloride (DXR) and thiazolyl blue tetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO).
Monoclonal rat anti-Gal-3 antibody was isolated from the supernatant of hybridoma (catalog number: TIB-166, American Type Culture Collection, Manassas, VA); customized polyclonal rabbit anti-Gal-3 antibody was created by Invitrogen (Carlsbad, CA); mouse anti-V5 was purchased from Invitrogen; polyclonal rabbit anti-Bax, mouse anti-Bcl-xl, mouse anti-p-ERK, mouse ERK1/2 and rabbit anti-p-Akt were purchased from Santa Cruz Biotechnology (Santa Cruz, CA); mouse anti-β-actin was purchased from Sigma-Aldrich; monoclonal mouse anti-Bax was purchased from BD Biosciences (San Diego, CA); mouse anti-p21, rabbit anti-Bcl2, rabbit anti-PARP (9542L), rabbit anti-cleaved caspase-3, rabbit anti-Akt and mouse anti-caspase-8 were purchased from Cell Signaling Technology (Beverly, MA); purified rabbit IgG was purchased from ZYMED (San Francisco, CA);

Harazono Y., Kho D.H., Balan V., Nakajima K., Zhang T., Hogan V, & Raz A. (2014). Galectin-3 leads to attenuation of apoptosis through Bax heterodimerization in human thyroid carcinoma cells. Oncotarget, 5(20), 9992-10001.

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