Potassium dichromate (K2Cr2O7) [CAS# 7778-50-9] was purchased from Sigma-Aldrich Chemical Co. St. Louis, Missouri, (USA). Ethanol (96%; CAS #: 64-17-5) was purchased from Merck Millipore (MS, USA). Creatinine (cat# CR 12 51), Urea (cat# UR 21 10) and catalase (cat# CA 25 17) colorimetric kits were both purchased from Bio Diagnostic (Giza, Egypt), while the ELISA kits, including nuclear factor erythroid 2-related factor 2 (Nrf2, cat# SL0985Ra), prostaglandin E2 (PGE2, cat# SL0601Ra), cyclooxygenase 2 (COX-2, cat# SL0218Ra), UDP-glucuronosyltransferase (cat# SL1171Ra), mitogen-activated protein kinase (MAPK) (cat# SL1529Ra), and extracellular signal-regulated kinase (ERK) (cat# SL1390Ra), were all obtained from (SUNLONG BIOTECH CO., Ltd.), Hangzhou, China.
Creatinine
Manufactured by Biodiagnostic
Sourced in Egypt
Creatinine is a laboratory test used to measure the amount of creatinine in the blood or urine. Creatinine is a waste product that is filtered out of the blood by the kidneys and excreted in the urine. The level of creatinine in the blood or urine can provide information about kidney function.
Automatically generated - may contain errors
Lab products found in correlation
Albumin, by Biodiagnostic (6 mentions)
Urea, by Biodiagnostic (4 mentions)
Aspartate aminotransferase, by Biodiagnostic (4 mentions)
Catalase, by Biodiagnostic (3 mentions)
Alkaline phosphatase, by Biodiagnostic (3 mentions)
Total protein, by Biodiagnostic (3 mentions)
Malondialdehyde, by Biodiagnostic (2 mentions)
Uric acid, by Biodiagnostic (2 mentions)
Glutathione peroxidase, by Biodiagnostic (2 mentions)
Reduced glutathione, by Biodiagnostic (2 mentions)
Potassium dichromate k2cr2o7, by Merck Group (1 mentions)
Ascorbic acid, by Honeywell (1 mentions)
Ursolic acid, by Merck Group (1 mentions)
Butylated hydroxytoluene, by Merck Group (1 mentions)
2 4 dinitrophenylhydrazine, by Merck Group (1 mentions)
Glutathione (gsh), by Merck Group (1 mentions)
Biozol reagent, by Thermo Fisher Scientific (1 mentions)
Syber green 1 step qrt pcr kit, by Thermo Fisher Scientific (1 mentions)
Folin ciocalteau reagent, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
17 protocols using creatinine
1
Antioxidant and Inflammation Evaluation
2
Antioxidant and Anti-inflammatory Assays
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Rutin (RU), gallic acid (GA), catechin, ursolic acid (UA), Folin–Ciocalteau reagent, 2,2 diphenyl-1-picrylhydrazyl (DPPH), 2,4 dinitrophenyl hydrazine (DNPH), 5, 5′, dithiobis-2-nitrobenzoic acid (DTNB), butylated hydroxytoluene (BHT), CCl4 (reagent grade, 99.9%), 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), NADPH, and GSH were acquired from Sigma-Aldrich, St Louis, MO, USA. Thiobarbituric acid (TBA) was gained from El-Nasr Pharmaceutical Chemicals Co. (Alex., Egypt). Ascorbic acid [(Asc): vitamin C] and Trolox were bought from Riedel-de Haën, Germany. Biozol reagent was purchased from Invitrogen, CA, USA. SYBER Green 1-step qRT-PCR Kit was purchased from Thermo Scientific, USA. Primers for tumor necrosis factor (TNF)-α, nuclear factor-kappa B (NF-κB), transforming growth factor (TGF)-β1, interleukin (IL)-6, and the tumor suppressor gene p53 were acquired from Bioneer, Korea. Kits for alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein (TP), albumin, creatinine, urea, triglycerides (TG), low-density lipoprotein cholesterol (LDL-c), and high-density lipoprotein cholesterol (HDL-c) were gained from Biodiagnostic, Cairo, Egypt.
3
Hepatoprotective and Antioxidant Evaluation
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Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein, albumin, creatinine, urea, and uric acids were purchased from Biodiagnostics Company (Dokki, Giza, Egypt). The RNA extraction Kit was purchased from Introbio Company, Cat.No. 17063. Reverse transcriptase Polymerase chain reaction (RT-PCR) sensiFAST™ cDNA synthesis kit (Bioline, United Kingdom). All used primers are commercially based. Aflatoxin from the Mycology and Mycotoxins Department, Animal Health Research Institute (ARC), Dokki, Egypt. Curcumin Nanoparticles (NT-Cur-NPs) were purchased from Nanotech Egypt for photo- electronics at the city of 6 October, Al Giza. Saccharomyces cerevisiae cell wall extract (100%, Beta-glucan: 20% minimum &Manan Oligosaccharides: 20% minimum.) was obtained from IBEX Group for animal nutrition and health products and produced by Shandong Bio Sunkeen Co., Ltd (a state-owned high-tech enterprise that integrates R&D, production and sales with rich experience of yeast and nature yeast products.
4
Ochratoxin-Induced Biochemical Alterations
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All chemicals used in this study were of analytical grade. Ochratoxin was purchased from Sigma Chemical Co. (St. Luis, MO, USA). Asp. Awamori powder was kindly supplied by Prof. K. Hayashi, Department of Biochemical Science and Technology, Faculty of Agriculture, Kagoshima University, Kagoshima, Japan. The kits of aspartate aminotransferase (AST), alkaline phosphatase (ALP), total protein, albumin, urea, creatinine, malondialdehyde (MDA), catalase (CAT), and total antioxidant capacity (TAC) were obtained from Biodiagnostics Co. (Cairo, Egypt). While LDH kit was purchased from Randox Laboratories Ltd. (Crumlin, UK), and CK-MB was obtained from Stanbio™ (CK-NAC [UV-Rate] kit; Boerne, TX, USA).
5
Fipronil Biochemical Assays in Toxicology
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Fipronil (Insecto SC 5%) is a product of BASF Company and manufactured by, Sinochem Group Ningbo Technical Co., Ltd., China. The assay kits used for biochemical measurements of aspartate aminotransferases (AST, EC 2.6.1.1.), alanine aminotransferases (ALT, EC 2.6.1.2), alkaline phosphatase (ALP, EC 3.1.3.1), lactate dehydrogenase (LDH, EC 1.1.1.27), superoxide dismutase (SOD, EC 1.15.1.1), catalase (CAT, EC 1.11.1.6), glutathione peroxidase (GPx, EC 1.11.1.9), glutathione-s-transferase (GST, EC 2.5.1.13), glutathione reduced (GSH), lipid peroxidation (LPO), albumin, uric acid and creatinine were purchased from Biodiagnostic Company, Dokki, Giza, Egypt. All other chemicals were of reagent grades and were obtained from reputed companies.
6
Serum and Urinary Biomarker Analysis
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Serum and urinary urea (Bio-diagnostic, Giza, Egypt, UR 21 10) and creatinine (Bio-diagnostic, Giza, Egypt, CR 12 51), and serum albumin (Bio-diagnostic, Giza, Egypt, AB 10 10) were assayed using commercial kits. Serum and urinary calcium and phosphorus levels (Egyptian Co. for Biotechnology – Spectrum Diagnostics, Al-Obour city, Egypt, 226,002, 294,002 for calcium and phosphorus, respectively) were estimated according to the o-cresolphthalein complex-one and UV-phosphomolybdate methods, respectively. creatinine clearance (CCr) was calculated according to the following equation: CCR (mL/min) = urinary creatinine (UCr; mg/dL) × urine flow (mL/min)/ creatinine in serum (mg/dL). Urine flow per min was estimated by dividing urine volume during 24 h by 1,440.
7
Cadmium Chloride-Induced Liver Damage
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Cadmium chloride (CdCl2) and Na2EDTA were purchased from Merck Company (Darmstadt, Germany). Aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, creatinine, superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) kits were purchased from Bio-Diagnostic Company (Cairo, Egypt). The other chemicals were purchased from the local chemical trade companies.
8
Cypermethrin Biochemical Evaluation Protocol
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Cypermethrin (95%) was obtained from Jiangsu Yangnong Chemical Co., Ltd., China. The assay kits used for biochemical measurements of total protein, albumin, urea nitrogen, aspartate aminotransferases (EC 2.6.1.1.), alanine aminotransferases (EC 2.6.1.2), alkaline phosphatase (EC 3.1.3.1), creatinine and gamma-glutamyl transferase (EC 2.3.2.2) in serum were purchased from Bio-Diagnostic Co., Egypt. Kit of total protein was obtained from Stanbio Laboratory, Texas, USA. All other chemicals were of reagent grades and obtained from Sigma-Aldrich and local scientific distributors in Egypt.
9
Biochemical Assays for Cell Viability and Apoptosis
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MTT assay kit and propidium iodide (PI) staining FC assay kit was purchased from Abcam (Boston, MA, USA). AST, ALT, and bilirubin (total and direct) kits and creatinine, urea kits, and GSH kit were all purchased from Biodiagnostics Company (Cairo, Egypt).
Heparanase ELISA kit (Abcam, Cambridge, UK), Annexin V/PI double staining kit (eBiosciencesDx), EGFR 96-well plate kit (Abcam, Cambridge, UK), uPA ELISA kit (Creative Diagnostics, New York, NY, USA), and BcL-2 ELISA kit (Zymed Laboratories, Carlsbad, CA, USA) were used.
Heparanase ELISA kit (Abcam, Cambridge, UK), Annexin V/PI double staining kit (eBiosciencesDx), EGFR 96-well plate kit (Abcam, Cambridge, UK), uPA ELISA kit (Creative Diagnostics, New York, NY, USA), and BcL-2 ELISA kit (Zymed Laboratories, Carlsbad, CA, USA) were used.
10
Biochemical and Hematological Assessments in Rats
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On the past day of the experimental period (42 days), rats were fasted overnight and then anesthetized with ether, and blood samples were drawn from the hepatic portal vein and then transported into centrifuge tubes. Tubes were centrifuged at 5000× g for 15 min at 23°C to collect serum for the biochemical examination. Serum samples were stored at −20°C until used for various biochemical analyses. To determine hematological parameters, additional blood samples (approximately 1 mL) were collected into test tubes containing EDTA.
The kits of urea, uric acid, creatinine, total proteins, albumin, Cystatin-C, neutrophil gelatinase-associated lipocalin (NGAL), sodium, potassium, superoxide dismutase (SOD), reduced GSH, nitric oxide (NO), malondialdehyde (MDA), and total antioxidant capacity (TAC) were obtained from Bio diagnostics Co. (Cairo, Egypt) whereas 8-hydroxy-2deoxyguanosine (8-OHdG) and enzyme-linked immunosorbent assay kits were utilized to measure the levels of caspase 3, nuclear factor kappa-B (NF-κB), tumor necrosis factor-alpha (TNF-α), and C-reactive protein (CRP) according to the manufacturer Kamiya Biomedical Co. (CA, USA).
The kits of urea, uric acid, creatinine, total proteins, albumin, Cystatin-C, neutrophil gelatinase-associated lipocalin (NGAL), sodium, potassium, superoxide dismutase (SOD), reduced GSH, nitric oxide (NO), malondialdehyde (MDA), and total antioxidant capacity (TAC) were obtained from Bio diagnostics Co. (Cairo, Egypt) whereas 8-hydroxy-2deoxyguanosine (8-OHdG) and enzyme-linked immunosorbent assay kits were utilized to measure the levels of caspase 3, nuclear factor kappa-B (NF-κB), tumor necrosis factor-alpha (TNF-α), and C-reactive protein (CRP) according to the manufacturer Kamiya Biomedical Co. (CA, USA).
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