Genomic mini ax stool spin

Manufactured by A&A Biotechnology

Sourced in Poland

The Genomic Mini AX Stool Spin is a lab equipment product designed for the extraction of genomic DNA from stool samples. It utilizes a spin column-based method to isolate DNA.

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Lab products found in correlation

16s metagenomic sequencing library preparation protocol, by Illumina (2 mentions)

2 protocols using genomic mini ax stool spin

16S Metagenomic Sequencing of Stool Samples

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Bacterial DNA was isolated using Genomic Mini AX Stool Spin (A&A Biotechnology, Gdynia, Poland), with several modifications, as described in our previous publication [22 (link)].
Libraries were prepared according to the Illumina 16S Metagenomic Sequencing Library Preparation protocol. After that, the libraries at a concentration 10 pM with 20% PhiX spike-in control were sequenced on Illumina MiSeq (Illumina, Inc., San Diego, CA, USA) using the V3 sequencing kit (300 bp paired-end reads) [22 (link)].

Mrozinska S., Kapusta P., Gosiewski T., Sroka-Oleksiak A., Ludwig-Słomczyńska A.H., Matejko B., Kiec-Wilk B., Bulanda M., Malecki M.T., Wolkow P.P, & Klupa T. (2021). The Gut Microbiota Profile According to Glycemic Control in Type 1 Diabetes Patients Treated with Personal Insulin Pumps. Microorganisms, 9(1), 155.

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16S rRNA Sequencing of Bacterial RNA

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Bacterial RNA was isolated using Genomic Mini AX Stool Spin (A&A Biotechnology, Gdynia, Poland), modified to include enzymatic treatment (lysozyme, lysostaphin and lyticase) and a bead-beating step. Libraries were prepared according to the Illumina 16S Metagenomic Sequencing Library Preparation protocol (https://support.illumina.com/content/dam/ /illumina-support/documents/documentation/chem-istry_documentation/16s/16s-metagenomic-libraryprep-guide-15044223-b.pdf). Briefly, universal external primers were used to amplify regions V3 and V4 of the 16S rRNA. After the polymerase chain reaction cleanup, samples were indexed, cleaned and pooled. Finally, 10 pM libraries with 10% PhiX Spike-In were sequenced on Illumina MiSeq (Illumina, Inc., San Diego, CA, US) using the V3 sequencing kit (300 bp paired-end reads).

Mrozińska S., Gosiewski T., Sroka-Oleksiak A., Szopa M., Bulanda M., Małecki M.T., & Klupa T. (2020). The effect of linagliptin treatment on gut microbiota in patients with HNF1A-MODY or type 2 diabetes — a preliminary cohort study. Clinical Diabetology, 8(6), 263-270.

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