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Scintillation cocktail

Manufactured by Merck Group
Sourced in United States, Germany

Scintillation cocktail is a liquid solution used in liquid scintillation counting, a technique employed to measure radioactivity. The cocktail contains organic compounds that emit light (scintillate) when excited by radiation, allowing the quantification of radioactive samples.

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2 protocols using scintillation cocktail

1

Steroid Hormone Extraction and Analysis

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Dexamethasone (internal standard), 4-Androstene-3,17-dione-2,3,4-13C3, Estrone-2,3,4-13C3, 17β-Estradiol-2,3,4-13C3, Testosterone-2,3,4-13C3, Anastrozole, Letrozole, KZ, protease from Bacillus licheniformis (subtilisin), formic acid, acetone, methanol, acetonitrile, hexane, methylene chloride, charcoal activated, Tris, HCl, KH2PO4, K2HPO4, KCl, NADPH, glycerol, BSA, scintillation cocktail, and Supel™-Select SPE HLB (six mL, 200 mg) columns were provided by Sigma-Aldrich (St. Louis, MO, USA). 1β-3H Androstene-3,17-dione (30 Ci/mmol) was obtained from Perkin-Elmer Life Science (Boston, MA, USA).
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2

Measuring Fatty Acid Oxidation in Adult Mouse Cardiomyocytes

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In adult mouse cardiomyocytes, FAO was measured according to the previously described method [21 (link)]. In brief, isolated adult cardiomyocytes were incubated with analysis medium containing fatty acids for 10 min, and at the end of the reaction the supernatant was added to 4 ml scintillation cocktail (Sigma-Aldrich, Germany) and radioactivity was measured using a scintillation counter [33 (link)].
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