Mouse monoclonal anti cx43

The antibodies used in this study were as follows: mouse monoclonal anti-Cx45 (MAB3100, Merck Millipore, Billerica, MA, USA), mouse monoclonal anti-Cx43 (C13720, BD Transduction Laboratories™, San Joes, CA, USA), goat polyclonal anti-actin (C-11, Santa Cruz Biotechnology, Santa Cruz, CA, USA), and goat anti-mouse IgG (H + L) HRP-conjugated (62-6520, Thermo Fisher Scientific, Rockford, IL, USA) antibodies. RNeasy^® Mini Kit (74104, Qiagen, Hilden, Germany), PrimeScript TM 1st strand cDNA kit (6110A, Takara Bio, Shiga, Japan), Taq polymerase PCR kit (DT95-E500, Solgent, Daejeon, Korea), tissue DNA Purification Kit (CME0112, Cosmogenetech, Daegu, Korea), T7 endonuclease 1 (T7E1) (M0302L, New England Biolabs, Ipswich, MA, USA), and LY (L0144, Sigma-Aldrich, St Louis, MO, USA) were purchased from local vendors.

The following primary antibodies were used in Western blot, Immunofluorescence and PLA analysis: mouse monoclonal anti-Cx43 (BD Transduction Laboratories™, USA, Cat. #610,062), rabbit polyclonal anti-Panx-1 (Sigma-Aldrich, USA, Cat. #AV42783-50UG), rabbit polyclonal anti-CALHM1 (Alomone Labs, Israel, Cat. #ACC-101), rabbit polyclonal anti-eNOS (BD Transduction Laboratories™, USA, Cat. #610,298), mouse monoclonal anti-eNOS (BD Transduction Laboratories™, USA, Cat. #610,296), rabbit monoclonal anti-nNOS (Cell Signaling Technology, Cat. #4231), rabbit polyclonal anti-iNOS (Millipore, Germany, Cat. #ab5383), rabbit polyclonal anti-GFAP (Sigma Aldrich, USA, Cat. #G9269), mouse monoclonal anti-GFAP (Sigma Aldrich, USA, #G3893), rabbit polyclonal anti-β-actin (Sigma Aldrich, USA, Cat. #A3853) and rabbit polyclonal anti-SNO-Cys (Sigma Aldrich, USA, Cat. #N5411).