Afp hs00173490 m1

Manufactured by Thermo Fisher Scientific

Sourced in United States

The AFP (Hs00173490_m1) is a gene expression assay designed to quantify the expression of the Alpha-Fetoprotein (AFP) gene. It is a molecular biology tool used for research purposes. The assay utilizes TaqMan® technology to enable sensitive and specific detection of the target transcript.

Automatically generated - may contain errors

Lab products found in correlation

7900 sequence detection system, by Thermo Fisher Scientific (1 mentions) Isospin cell tissue rna, by Nippon Gene (1 mentions) Rneasy mini kit, by Qiagen (1 mentions) 18s hs99999901 s1, by Thermo Fisher Scientific (1 mentions) 7900ht sequence detection system, by Thermo Fisher Scientific (1 mentions)

2 protocols using afp hs00173490 m1

Quantitative Analysis of FOXM1 and AFP Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was extracted using ISOSPIN Cell & Tissue RNA (Nippon Gene Co., Ltd., Tokyo, Japan) according to the manufacturer’s instructions. Quantitative PCR probes for FOXM1 (Hs1073586_m1) and AFP (Hs00173490_m1) were purchased from Applied Biosystems (Foster City, CA, USA). The expression of selected genes was determined in triplicate using the 7900 Sequence Detection System (Applied Biosystems). Each sample was normalized relative to the expression of a reference gene (18S rRNA). Quantitation of genes expressed in cell lines relative to Huh7 cells was performed using the ΔΔCT method.

Li R., Okada H., Yamashita T., Nio K., Chen H., Li Y., Shimakami T., Takatori H., Arai K., Sakai Y., Yamashita T., Mizukoshi E., Honda M, & Kaneko S. (2022). FOXM1 Is a Novel Molecular Target of AFP-Positive Hepatocellular Carcinoma Abrogated by Proteasome Inhibition. International Journal of Molecular Sciences, 23(15), 8305.

+ Open protocol

+ Expand

Quantitative Analysis of Liver Genes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was extracted using an RNeasy Mini Kit (QIAGEN, Valencia, CA)
according to the manufacturer’s instructions. The expression of selected
genes was determined in triplicate using the Applied Biosystems 7900HT Sequence
Detection System (Applied Biosystems, Foster City, CA) and the
−ΔΔCT method. The following probes were used:
AFP, Hs00173490_m1; FOXM1, Hs01073586_m1;
OATP1B3, Hs00251986_m1; CYP3A4,
Hs00430021_m1; and 18S, Hs99999901_s1 (Applied Biosystems).

Yamashita T., Kitao A., Matsui O., Hayashi T., Nio K., Kondo M., Ohno N., Miyati T., Okada H., Yamashita T., Mizukoshi E., Honda M., Nakanuma Y., Takamura H., Ohta T., Nakamoto Y., Yamamoto M., Takayama T., Arii S., Wang X.W, & Kaneko S. (2014). Gd-EOB-DTPA-enhanced Magnetic Resonance Imaging and Alpha-fetoprotein Predict Prognosis of Early-Stage Hepatocellular Carcinoma. Hepatology (Baltimore, Md.), 60(5), 1674-1685.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!