All chemicals and reagents were purchased from Sigma Aldrich (St. Louis, MO, United States) unless otherwise mentioned. For culturing of cells, tissue culture grade plastic ware was purchased from BD Biosciences (Bedford, MA, United States). RPMI-1640 media and fetal bovine serum (FBS) were purchased from GIBCO (Grand Island, NY, United States) for cell culture. L-pyruvate, L-glutamine, streptomycin and penicillin were from Serva (Heidelberg, Germany). Anti–mouse fluorochrome labeled antibodies (Abs): CD4-PB, CD62L-APC, CD44-PerCP-Cy5.5, CD127-PE, and Abs for ELISA were procured from BD Pharmingen (San Diego, CA, United States) or otherwise mentioned.
Tissue culture grade plastic ware
Manufactured by BD
Sourced in United States
Tissue culture grade plastic-ware is a specialized laboratory equipment designed for the cultivation and maintenance of cells, tissues, and organs in a controlled environment. These products are manufactured to meet the precise requirements of cell culture applications, ensuring a consistent and optimal growth environment for various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Cd44 percp cy5, by BD (3 mentions)
Cd62l apc, by BD (3 mentions)
Rpmi 1640, by Thermo Fisher Scientific (3 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions)
Cd127 pe, by BD (2 mentions)
Cd4 pb, by BD (2 mentions)
Abs for elisa, by BD (2 mentions)
Klrg1 pe, by BD (2 mentions)
Cd8 apc cy7, by BD (2 mentions)
Streptomycin, by Serva Electrophoresis (1 mentions)
Penicillin, by Serva Electrophoresis (1 mentions)
Rpmi 1640 media, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Serva Electrophoresis (1 mentions)
L pyruvate, by Serva Electrophoresis (1 mentions)
Fetal calf serum (fcs), by Thermo Fisher Scientific (1 mentions)
Cd4 fitc, by BD (1 mentions)
Ifn γ pe cy7, by BD (1 mentions)
Tnfα percpcy5, by BD (1 mentions)
Cxcr3 fitc, by BioLegend (1 mentions)
Cd27 fitc, by BioLegend (1 mentions)
4 protocols using tissue culture grade plastic ware
1
Mouse T Cell Phenotyping Protocol
2
Detailed Protocols for Immune Cell Analysis
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All standard chemicals and reagents used in the study were purchased from Sigma (St. Louis, Mo., USA), and fluorochrome labeled Abs to CD4-FITC, CD8-APCCy7, CD44-PerCPCy5.5, CD62L-APC and KLRG1-PE were obtained from BD Biosciences (San Diego, CA), unless indicated. RPMI-1640 and FCS were purchased from GIBCO (Grand Island, NY). For culturing of cells, tissue culture grade plastic-ware was purchased from BD Biosciences (Bedford, MA). Purified protein derivative (PPD) of Mtb was prepared by standard protocols, as described elsewhere [47 (link), 48 (link)].
3
Murine Lymphocyte Immunophenotyping
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The chemicals and reagents were purchased from Sigma Aldrich (St. Louis, MO). Anti–mouse flurochrome labeled Abs: CD4-PB, CD62L-APC, CD44-PerCP-Cy5.5, CD127-PE, FoxP3-FITC, PD1-PECy7, CCR7-APC, IFN-γ-PECy7, TNFα-PerCPCy5.5, IL-17-PerCPCy5.5 and Abs for ELISA were procured from BD Pharmangin (San Diego, CA) or otherwise mentioned. RPMI-1640 and FBS were purchased from GIBCO (Grand Island, NY). Tissue culture grade plastic-ware was purchased from BD Biosciences (Bedford, MA).
4
Detailed Immunophenotyping Assay Protocol
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Chemicals and reagents were purchased from Sigma Aldrich (St. Louis, MO). Anti–mouse flurochrome labeled antibodies (Abs): CD4-PB, CD8-APC-Cy7, CD62L-APC, CD44-PerCP-Cy5.5, CD127-PE, KLRG1-PE, IFN-γ-PECy7, TNFα-PerCPCy5.5, IL-17-PerCPCy5.5 and Abs for ELISA were procured from BD Pharmingen (San Diego, CA); CD27-FITC, CD43-PE, CXCR3-FITC and CCR6-APC from Biolegend (San Diego, CA) or otherwise mentioned. RPMI-1640 and FBS were purchased from GIBCO (Grand Island, NY). For culturing of cells, tissue culture grade plastic-ware was purchased from BD Biosciences (Bedford, MA).
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