Filtration system

Manufactured by Merck Group

Sourced in United States

The Filtration system is a laboratory equipment designed to separate solid particles from a liquid or gaseous mixture. It uses a porous medium, such as a filter paper or membrane, to trap the solid particles while allowing the fluid to pass through. The core function of this system is to purify or clarify the fluid by removing unwanted contaminants, depending on the pore size of the filter.

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Lab products found in correlation

Close spelling variants of this product

Milli-Q filtration system (41 citations) Milli-Q water filtration system (25 citations) Tangential flow filtration system (10 citations) Vacuum filtration system (9 citations) Millipore filtration system (8 citations) Labscale tangential flow filtration system (7 citations) Milli-Q-UF filtration system (4 citations) Multiscreen Filtration System (4 citations) 0.22 μm sterile filtration systems (3 citations) Milli-Q filtration/purification system (3 citations)

19 protocols using filtration system

Mass Spectrometry Reagent Preparation

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Methanol (MeOH), acetonitrile (ACN), formic acid (FA), ammonium bicarbonate and glacial acetic acid were purchased from Fisher Scientific (Pittsburgh, PA). Borane pyridine, formaldehyde, and deuterium formaldehyde were from Sigma-Aldrich (St. Louis, MO). 2,5-dihydroxybenzoic acid (DHB) was obtained from Acros Organics (Morris Plains, NJ), and alpha-cyano-4-hydroxycinnamic acid (CHCA) was purchased from Sigma-Aldrich (St. Louis, MO). Acidified methanol was prepared as 90% methanol, 9% water and 1% glacial acetic acid (v/v/v). All water used in this study was doubly distilled on a Millipore filtration system (Burlington, MA) or Fisher HPLC grade.

Liu Y., Buchberger A.R., DeLaney K., Li Z, & Li L. (2019). Multifaceted Mass Spectrometric Investigation of Neuropeptide Changes in Atlantic Blue Crab, Callinectes sapidus, in Response to Low pH Stress. Journal of proteome research, 18(7), 2759-2770.

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Preparation and Analysis of MK and BBR

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Methanol (purity ≥ 99.9%) was used for sample preparation and was purchased from Sigma-Aldrich (Milan, Italy). Formic acid (purity ≥ 95%) and acetonitrile (purity ≥ 99.9%) were used to prepare the mobile phases for HPLC analyses and were purchased from Sigma-Aldrich (Milan, Italy), while water was Milli-Q grade (18.2 MΩ.cm at 25 °C) and was obtained through a Millipore filtration system (Billerica, MA, US). MK and BBR chloride dihydrate standards were purchased from Sigma-Aldrich (Milan, Italy).

Marcheluzzo S., Faggian M., Zancato M, & Peron G. (2021). Analysis of Monacolins and Berberine in Food Supplements for Lipid Control: An Overview of Products Sold on the Italian Market. Molecules, 26(8), 2222.

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Synthesis and Purification of MWNTs

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The MWNTs were synthesized using a floating catalytic chemical vapor deposition process using ferrocene and ethylene as the transition metal and carbon precursors, respectively. After the synthesis, the MWNTs were submitted to a simple purification process by washing and filtering several times with isopropyl alcohol in a Millipore filtration system to remove any non-reacted ferrocene and other carbon impurities. After the cleaning process, the MWNTs were dried at 80 °C for 12 h.

Munk M., Ladeira L.O., Carvalho B.C., Camargo L.S., Raposo N.R., Serapião R.V., Quintão C.C., Silva S.R., Soares J.S., Jorio A, & Brandão H.M. (2016). Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes. Scientific Reports, 6, 33588.

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Preparation of Anhydrous Solvents

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Tetrahydrofuran (THF), toluene and ethanol were distilled from sodium benzophenone ketyl, calcium hydride and magnesium, respectively, under nitrogen immediately prior to use. Water was purified by a Millipore filtration system. Other chemicals were purchased from Aldrich and used as received without further purification.

Lou X., Hong Y., Chen S., Leung C.W., Zhao N., Situ B., Lam J.W, & Tang B.Z. (2014). A Selective Glutathione Probe based on AIE Fluorogen and its Application in Enzymatic Activity Assay. Scientific Reports, 4, 4272.

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Oxaliplatin Formulation and Vesicle Incubation Buffer

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Oxaliplatin powder (Actavis New Zealand) was dissolved in 5% glucose solution, sonicated and filtered with a 0.22 μm Millipore filtration system (Bedford, USA) and stored at -20°C. The membrane vesicle incubation buffer solution consisted of 50 mM MOPS-Tris, 70 mM KCl, 7.5 mM MgCl₂ and was freshly prepared using MOPS-Tris, KCl and MgCl₂ powders dissolved in Milli-Q grade water (Millipore, Bedford, USA). All other chemicals were sourced from Sigma-Aldrich, St Louis, MO, USA. Stock solutions of 5(6)-carboxy-2,'7'-dichlorofluorescein (CDCF), myricetin and MK571 were prepared at concentrations 1000 times higher than working solutions in dimethylformamide and stored at -20°C.

Myint K., Li Y., Paxton J, & McKeage M. (2015). Multidrug Resistance-Associated Protein 2 (MRP2) Mediated Transport of Oxaliplatin-Derived Platinum in Membrane Vesicles. PLoS ONE, 10(7), e0130727.

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Fabrication of Self-Assembled Monolayers

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Deionized water (18.2 MΩ·cm)
was obtained from a Millipore filtration system. Glass substrates
were cleaned by oxygen plasma before use. Octadecanethiol (ODT, 98%),
16-mercaptohexadecanoic acid (MHDA), Cu(ClO₄)₂, polystyrene spheres (500 nm, 10 wt % in water, Product no. 59769,
Sigma-Aldrich) and methylene blue (MB, 99%) were purchased from Aldrich.
Acetone (99.5% purity) and absolute ethanol (99.5% purity) were used
as received from VWR without further purification.

Luo S., Mancini A., Wang F., Liu J., Maier S.A, & de Mello J.C. (2022). High-Throughput Fabrication of Triangular Nanogap Arrays for Surface-Enhanced Raman Spectroscopy. ACS Nano, 16(5), 7438-7447.

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Synthetic Protocols and Analytical Techniques

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All chemicals and solvents were purchased from commercial sources and used without further purification. Solvents were purified by standard procedures. The water was purified by a Millipore filtration system. All chemical reactions were detected by thin-layer chromatography under 254 nm (or 365 nm) UV lamp. ¹H and ¹³C NMR spectra were recorded on a WIPM 400 spectrometer using tetramethylsilane (TMS) as the internal standard. High-resolution mass (HRMS) spectra were recorded on a Waters LCT Premier XE spectrometer using standard conditions (ESI, 70 eV). All absorption spectrums were measured on a UV-1800 UV visible spectrophotometer. All fluorescence spectra were measured on an FL-4500 fluorescence spectrometer. The pH measurements were taken on a METTLER TOLEDO FiveEasy Plus pH meter. The cell imaging experiments were taken under an Olympus IX71 inverted fluorescence microscope.

Du M., Zhang Y., Xu Z., Dong Z., Zhao S., Du H, & Zhao H. (2023). Point-of-Care and Dual-Response Detection of Hydrazine/Hypochlorite-Based on a Smart Hydrogel Sensor and Applications in Information Security and Bioimaging. Molecules, 28(9), 3896.

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Gold Nanorod Synthesis and Collagen Extraction

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Cetyltrimethyl ammonium bromide (CTAB), chloroauric acid trihydrate (HAuCl₄.3H₂O), ascorbic acid, sodium borohydride (NaBH₄), hydrochloric acid (HCl), and silver nitrate (AgNO₃) were purchased from Millipore Sigma (St. Louis, MO) for gold nanorod (GNR) synthesis. Acetic acid (glacial), sodium chloride, sodium phosphate, sodium hydroxide, and citric acid were purchased from Millipore Sigma for collagen extraction and purification. All solutions were freshly prepared in nanopure water (resistivity ~ 18.2 MΩ-cm, Millipore filtration system, Darmstadt, Germany). To maintain reagent integrity: HAuCl₄·3H₂O was kept at 0°C, NaBH₄ and AgNO₃ solids were stored under vacuum, HAuCl₄ and AgNO₃ solutions were kept at 4°C, and CTAB solutions were maintained at 27°C and 100 rpm. Other reagents were stored at room temperature. Rat tails and mouse cadavers were obtained from the ASU Department of Animal Care Technologies. BALB/c mice were purchased at 6-8 weeks from Charles River Laboratories (Wilmington, MA, USA). Commercially-available 6-0 polyglycolic acid (PGA) sutures were purchased from CARA Life, Inc. (Rancho Cucamonga, CA) and 6-0 silk sutures from Ethicon (Edinburgh, United Kingdom). All suture sizes are reported based on those in the United States Pharmacopeia.

Ghosh D., Urie R., Chang A., Nitiyanandan R., Lee J.K., Kilbourne J, & Rege K. (2019). Light-Activated Tissue-Integrating Sutures as Surgical Nanodevices. Advanced healthcare materials, 8(14), e1900084.

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Synthesis of Deuterated Organic Compounds

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Methyl propiolate (MP, >98.0%), methyl ethynyl ketone (MEK, >97.0%), 1,4-diazabicyclo[2.2.2]octane (DABCO, >98.0%), and trimethylolpropane (>98.0%) were purchased from Tokyo Chemical Industry Co, Ltd. Heavy water (D₂O, 99.9 atom% D) and heavy oxygen water (H₂¹⁸O, 98 atom % ¹⁸O), and propiolic acid (98%) were purchased from Energy Chemical. THF (inhibitor-free, HPLC grade, ≥99.9%) and DCM (HPLC grade, ≥99.9%) were purchased from Sigma-Aldrich. Water was purified with a Millipore filtration system.

Song B., Zhang J., Zhou J., Qin A., Lam J.W, & Tang B.Z. (2023). Facile conversion of water to functional molecules and cross-linked polymeric films with efficient clusteroluminescence. Nature Communications, 14, 3115.

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Silk Fibroin Extraction and Purification

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Silkworm (Bombyx mori) cocoons were purchased from Mulberry Farms as a source of silk fibroin protein (henceforth referred to as silk). Sodium carbonate (Na₂CO₃), and lithium bromide (LiBr) were purchased from Millipore Sigma for silk fibroin extraction from silkworm cocoons. Dialysis bags, 3.5 kDa molecular weight cut‐off (Spectra/Por), were purchased from Fisher Scientific to facilitate purification of silk fibroin. ICG dye was purchased from MP Biomedicals (#ICN15502050) and stored at 4°C. All solutions were freshly prepared in nanopure water (NPW; resistivity ~18.2 MΩ cm; Millipore Filtration System). BALB/c mice were purchased at ~10 weeks from Charles River Laboratories. Commercially available 4‐0 Monosof™ Monofilament Nylon Sutures (Medtronic) were purchased from esutures.com.

Ghosh D., Salinas C.M., Pallod S., Roberts J., Makin I.R., Yaron J.R., Witte R.S, & Rege K. (2022). Temporal evaluation of efficacy and quality of tissue repair upon laser‐activated sealing. Bioengineering & Translational Medicine, 8(2), e10412.

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