Fluvastatin capsules were purchased from Novartis Pharmaceutical Co., Ltd. (Beijing, China). A Masson staining kit was purchased from Leagene Biotechnology Co., Ltd. (Beijing, China). A hematoxylin-eosin staining kit was purchased from Servicebio Technology Co., Ltd. (Wuhan, China). Cell Counting Kit-8 (CCK-8) was obtained from Fcmacs Biotech Co., Ltd. (Nanjing, China). A BCA protein assay kit was purchased from Beyotime Biotechnology Co., Ltd. (Shanghai, China). Rosiglitazone (RSG, selective PPAR-γ agonist) and T0070907 (selective PPAR-γ antagonist) were obtained from Selleck Chemicals (Houston, USA). LPS was purchased from Sigma Chemical (St. Louis, USA). Recombinant rat IL-4 and INF-γ were purchased from PeproTech Inc. (New Jersey, USA). ELISA kits (IL-1, IL-6, TNF-α, IL4, IL-10, IL-13, and TGF-β1) were obtained from JinYiBai Biological Technology (Nanjing, China). The antibodies (JAK2, STAT6, and p-STAT1) were purchased from Santa Cruz Biotechnology (California, USA). The antibodies (PPAR-γ, STAT1, and p-PPAR-γ) were purchased from Bioss (Beijing, China). The antibodies (SOCS1, SOCS3, p-JAK2, and p-STAT6) were purchased from Affinity Biosciences. APC-anti-mouse CD86 was purchased from BioLegend (San Diego, USA). PE-Cy7-anti-mouse CD206 and Intracellular Fixation & Permeabilization set were purchased from Thermo Fisher Scientific (Massachusetts, USA).
P stat6
Manufactured by Affinity Biosciences
Sourced in China
The P-STAT6 is a laboratory equipment designed to measure the phosphorylation level of STAT6 protein. STAT6 is a transcription factor involved in various cellular processes. The P-STAT6 equipment provides a quantitative assessment of STAT6 phosphorylation status in biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Beyotime (2 mentions)
Rosiglitazone, by Selleck Chemicals (1 mentions)
P stat1, by Santa Cruz Biotechnology (1 mentions)
Apc anti mouse cd86, by BioLegend (1 mentions)
Stat6, by Santa Cruz Biotechnology (1 mentions)
T0070907, by Selleck Chemicals (1 mentions)
Hematoxylin eosin staining kit, by Wuhan Servicebio Technology (1 mentions)
Recombinant rat il 4, by Thermo Fisher Scientific (1 mentions)
Pparγ, by Bioss Antibodies (1 mentions)
P ppar γ, by Bioss Antibodies (1 mentions)
Socs1, by Affinity Biosciences (1 mentions)
P jak2, by Affinity Biosciences (1 mentions)
Lipopolysaccharides (lps), by Merck Group (1 mentions)
Ecl reagent, by 7Sea Biotech (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
β actin, by Proteintech (1 mentions)
Phenyl methyl sulfonyl fluoride (pmsf), by Beyotime (1 mentions)
Ab76302, by Abcam (1 mentions)
Af7021, by Affinity Biosciences (1 mentions)
S0001, by Affinity Biosciences (1 mentions)
3 protocols using p stat6
1
Macrophage Polarization Modulation
2
Protein Extraction and Western Blot Analysis
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RIPA lysis buffer (Beyotime, Shanghai, China) with 1% PMSF (Beyotime) was utilized for total protein extraction. The proteins were denatured at a high temperature and separated by SDS-PAGE. The primary antibodies used are as follows: SOX11 (1:1000; Affinity, Changzhou, China), p-STAT6 (1:1000; Affinity), STAT6 (1:500; Affinity), and β-actin (1:2000; Proteintech, Wuhan, China). Antibody binding was visualized using secondary antibodies (Proteintech). The blots were analyzed using Gel-Pro-Analyzer software with the ECL reagent (7sea biotech, Shanghai, China).
3
Penumbral Protein Expression Analysis
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Total protein of the ischemic penumbra cortex (n = 4/group) was extracted according to the previous method (Cai et al., 2022) ; protein concentrations were determined using a BCA Protein Assay Kit (Beyotime, Shanghai, China). Proteins were separated using 10% SDS-PAGE gels and then transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Massachusetts, USA). Blocked with 5% BSA for 1 h then incubated with primary antibody overnight at 4 °C, STAT6 (1:500 dilution, DF6302, Affinity); p-STAT6 (1:500 dilution, DF3301, Affinity); PPARc (1:500 dilution, DF6284, Affinity); p-PPARc, (1:500 dilution, DF3284, Affinity); p-NF-jB p65 (1:1000 dilution, ab76302, Abcam); GAPDH (1:1000 dilution, AF7021, Affinity). Subsequently, incubated with secondary antibody (1:5000 dilution, S0001, Affinity) for 1 h. Visualized using an Ultra Hypersensitive ECL Chemiluminescence Kit (Beyotime), chemiluminescence signals were photographed with ChampGel imager and Sage capture software (Sage, Beijing, China). The relative intensity of the protein bands was calculated using Ima-geJ software.
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