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N fluorenyl 9 methoxycarbonyl fmoc protected l amino acids

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N-fluorenyl-9-methoxycarbonyl (Fmoc) protected L-amino acids are a class of amino acid derivatives used in solid-phase peptide synthesis. They provide a temporary protecting group for the amino terminus of the amino acid during chemical reactions, ensuring site-specific incorporation into peptide sequences.

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Lab products found in correlation

Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (3 mentions) Penicillin streptomycin, by Merck Group (3 mentions) Methanol, by Thermo Fisher Scientific (3 mentions) Acetonitrile, by Thermo Fisher Scientific (3 mentions) Fetal bovine serum (fbs), by Atlanta Biologicals (3 mentions) 1 palmitoyl 2 oleoyl sn glycero 3 phosphocholine, by Avanti Polar Lipids (3 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (2 mentions) Dichloromethane, by Thermo Fisher Scientific (2 mentions) Trifluoroacetic acid, by Thermo Fisher Scientific (2 mentions) N dimethylformamide, by Thermo Fisher Scientific (2 mentions) N tetramethyluronium hexafluorophosphate, by GL Biochem (2 mentions) Pierce ldh cytotoxicity assay kit, by Thermo Fisher Scientific (1 mentions) N n dimethylformamide (dmf), by Thermo Fisher Scientific (1 mentions) Alexa fluor 750 nhs ester, by Thermo Fisher Scientific (1 mentions) Methyl thiazolyl tetrazolium (mtt), by Thermo Fisher Scientific (1 mentions) Triisopropylsilane tis, by Merck Group (1 mentions) O benzotriazole n n n n tetramethyl uronium hexafluorophosphate hbtu, by GL Biochem (1 mentions) 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide, by Merck Group (1 mentions) Cellevent caspase 3 7 green detection reagent, by Thermo Fisher Scientific (1 mentions) Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Fmoc-protected amino acids (28 citations) Fmoc-amino acids (27 citations) N-Fmoc-amino acids (3 citations) Amino acids (3 citations) Protected amino acids (2 citations)

5 protocols using n fluorenyl 9 methoxycarbonyl fmoc protected l amino acids

1

Synthesis and Characterization of Antibody-Drug Conjugates

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N-Hydroxybenzotriazole (HOBt), o-benzotriazol-N,N,N,N′,N′-tetramethyluronium hexafluorophosphate (HBTU), and all N-fluorenyl-9-methoxycarbonyl (Fmoc) protected L-amino acids were purchased from GL Biochem Ltd. H-Rink Amide-ChemMatrix solid support resin was purchased from PCAS BioMatrix Inc. Diisopropylehtylamine (DIEA), piperazine, N,N-dimethylformamide (DMF), dichloro-methane (DCM), trifluoroacetic acid (TFA), methanol, acetonitrile, dimethyl sulfoxide (DMSO), and Dulbecco’s modified Eagle’s medium (DMEM) were all from Thermo Fisher Scientific Inc. Fetal bovine serum (FBS) was purchased from Atlanta Biologicals Inc. Penicillin–streptomycin was purchased from Sigma-Aldrich. Pyridyldithiol-activated Py-ds-Prp-monomethylauristatin F (MMAF) was purchased from Concortis. 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) was from Avanti Polar Lipids Inc. 3-(4,5-Dimethylth-iazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from EMD Millipore. Pierce LDH Cytotoxicity Assay Kit was purchased from Thermo Fisher Scientific Inc. Noncanonical amino acids (Gla and Aad) were purchased from ChemImpex.
Burns K.E., Hensley H., Robinson M.K, & Thévenin D. (2017). Therapeutic Efficacy of a Family of pHLIP–MMAF Conjugates in Cancer Cells and Mouse Models. Molecular pharmaceutics, 14(2), 415-422.
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2

Antibody-Drug Conjugate Synthesis

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N-Hydroxybenzotriazole (HOBt), o-benzotriazol-N, N, N, N’, N’-tetramethyluronium hexafluorophosphate (HBTU), and all N-fluorenyl-9-methoxycarbonyl (Fmoc) protected L-amino acids were purchased from GL Biochem Ltd. H-Rink Amide-ChemMatrix solid support resin was from PCAS BioMatrix Inc. Diisopropylehtylamine (DIEA), piperazine, N, N-dimethylformamide (DMF), dichloromethane (DCM), trifluoroacetic acid (TFA), methanol, acetonitrile, dimethyl sulfoxide (DMSO) and Dulbecco’s modified Eagle’s medium (DMEM) were all from Thermo Fisher Scientific Inc. Fetal Bovine Serum (FBS) was purchased from Atlanta Biologicals Inc. Penicillin-Streptomycin was purchased from Sigma-Aldrich. monomethylauristatin E (MMAE), and pyridyldithiol-activated MMAE (Py-ds-Prp-MMAE) were from Concortis. 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) was from Avanti Polar Lipids Inc. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from EMD Millipore. AlexaFluor 750 NHS ester was purchased from Life Technologies.
Burns K.E., Robinson M.K, & Théveninr D. (2015). Inhibition of Cancer Cell Proliferation and Breast Tumor Targeting of pHLIP-Monomethyl Auristatin E Conjugates. Molecular pharmaceutics, 12(4), 1250-1258.
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3

Peptide Synthesis and Cytotoxicity Evaluation

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N-fluorenyl-9-methoxycarbonyl (Fmoc)-protected L-amino acids, piperdine Rink-Amide resin (100-200 mesh, loading: 0.51 mmol g-1), diisopropylethylamine (DiEA) and o-benzotriazole-N,N,N',N- tetramethyluroniumhexafluorophosphate (HBTU) were provided by GL Biochem Ltd. (Shanghai, China). HAuCl4, Diisopropylethylamine (DIEA), dimethylformamide (DMF), trifluoroacetic acid (TFA) and piperdine were obtained by Shanghai Reagent Chemical Co. (China). Triisopropylsilane (TIS) was purchased from Sigma-Aldrich (USA). MTT, Dulbecco's modified Eagle's medium (DMEM), trypsin, fetal bovine serum (FBS), Penicillin-streptomycin were provided by GIBCO Invitrogen Corp. All other reagents were used without further purification.
Zhang W., Cai K., Li X., Zhang J., Ma Z., Foda M.F., Mu Y., Dai X, & Han H. (2019). Au Hollow Nanorods-Chimeric Peptide Nanocarrier for NIR-II Photothermal Therapy and Real-time Apoptosis Imaging for Tumor Theranostics. Theranostics, 9(17), 4971-4981.
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4

Peptide Synthesis and Cell Viability Assays

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N-Hydroxybenzotriazole (HOBt), o-benzotriazol-N, N, N, N′, N′-tetramethyluronium hexafluorophosphate (HBTU), and all N-fluorenyl-9-methoxycarbonyl (Fmoc) protected L-amino acids were purchased from GL Biochem Ltd. H-Rink Amide-ChemMatrix solid support resin was purchased from PCAS BioMatrix Inc., Diisopropylehtylamine (DIEA), Piperazine, N, N-dimethylformamide (DMF), dichloromethane (DCM), trifluoroacetic acid (TFA), methanol, acetonitrile, dimethyl sulfoxide (DMSO), and Dulbecco’s modified Eagle’s medium (DMEM) were all purchased from Thermo Fisher Scientific Inc. Fetal Bovine Serum (FBS) was purchased from Atlanta Biologicals Inc. Penicillin-Streptomycin was purchased from Sigma-Aldrich. 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) was purchased from Avanti Polar Lipids Inc. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from EMD Millipore. Mitochondrial Membrane Potential/Annexin V Apoptosis Kit, Pierce LDH Cytotoxicity Assay Kit, and CellEvent™ Caspase-3/7 Green Detection Reagent were all purchased from Thermo Fisher Scientific.
Burns K.E., McCleerey T.P, & Thévenin D. (2016). pH-Selective Cytotoxicity of pHLIP-Antimicrobial Peptide Conjugates. Scientific Reports, 6, 28465.
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5

Peptide Synthesis Using HBTU and HOBt

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N-Hydroxybenzotriazole (HOBt), o-benzotriazol-N, N, N, N′, N′-tetramethyluronium hexafluorophosphate (HBTU), and all N-fluorenyl-9-methoxycarbonyl (Fmoc) protected L-amino acids were purchased from GL Biochem Ltd. H-Rink Amide-ChemMatrix solid support resin was purchased from PCAS BioMatrix Inc. Diisopropylehtylamine (DIEA), piperazine, N, N-dimethylformamide (DMF), dichloromethane (DCM) and trifluoroacetic acid (TFA) were from Thermo Fisher Scientific Inc. All lipids were purchased from Avanti Polar Lipids (Alabaster, AL). 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phospholcholine (POPC), 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphoethanolamine (POPE), 1‐palmitoyl‐2‐oleoyl‐sn‐glycero‐3‐phosphoserine (POPS).
Vasquez-Montes V., Gerhart J., King K.E., Thévenin D, & Ladokhin A.S. (2017). Comparison of lipid-dependent bilayer insertion of pHLIP and its P20G variant. Biochimica et biophysica acta, 1860(2), 534-543.
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