The colorectal tissue whole protein was extracted using a protein lysis buffer (P0013B, Beyotime, China). 50 μg of total protein was separated by 10% SDS‐polyacrylamide gel electrophoresis and then transferred onto 0.22 μm polyvinylidene fluoride membrane. Then blocked with 5% nonfat milk powder in TBST for 2 h at room temperature and incubated with the appropriate primary antibody at 4°C overnight. The primary antibodies and their dilution concentration were as follows: anti‐SR‐B1 (1:1000, NB400‐104, Novus Biologicals, USA), anti‐LDL‐R (1:1000, K009497P, Solarbio, China), anti‐PD‐L1 (1:1000, K009918P, Solarbio, China), anti‐GAPDH (1:2500, E12‐052, EnoGene, China), anti‐ABCA1 (1:1000, ab18180, Abcam, UK), anti‐HLA‐B (1:1000, K009916P, Solarbio, China), and anti‐β‐Actin (1:2500, #21338, SAB, USA). Subsequently, the membranes were washed with 1 × TBST and incubated with secondary antibodies: Goat anti‐Mouse or anti‐Rabbit IgG (1:10000) for 1 h at room temperature. After the membranes were washed again by 1 × TBST buffer, the proteins were visualized using enhanced chemiluminescence.
E12 052
Manufactured by Enogene
Sourced in China
The E12-052 is a laboratory equipment product manufactured by Enogene. It is a versatile piece of equipment designed for use in various scientific applications. The core function of the E12-052 is to perform precise measurements and analyses, but its specific intended use is not provided in this factual and unbiased description.
Automatically generated - may contain errors
Lab products found in correlation
Ab18180, by Abcam (1 mentions)
Nb400 104, by Novus Biologicals (1 mentions)
Ab65783, by Abcam (1 mentions)
Ab5076, by Abcam (1 mentions)
Ab18258, by Abcam (1 mentions)
Sc 47778, by Santa Cruz Biotechnology (1 mentions)
Sc 271390, by Santa Cruz Biotechnology (1 mentions)
New super ecl kit, by Keygen Biotech (1 mentions)
Ab108319, by Abcam (1 mentions)
2 protocols using e12 052
1
Protein Expression Analysis in Colorectal Tissue
2
Hippocampal Protein Expression Analysis
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The animals were sacrificed for experiments at the following ages: postnatal day 11 (PND-11), PND-16, PND-21, PND-35, and PND-67, with bilateral hippocampal tissues meticulously isolated, followed by homogenization on ice with lysate buffer plus protease inhibitors. Following centrifugation at 12,000 r/min for 15 min at 4°C, the resultant supernatants were measured for protein concentrations with a BCA assay kit, with bovine serum albumin serving as a standard, and were transferred onto polyvinylidene fluoride membranes and blocked with blocking buffer for 1 h at r/t, followed by incubation with primary antibodies added to DCX (dilution 1:1000, sc-271390, Santa Cruz), Iba1 (dilution 1:500, ab5076, Abcam), BDNF (dilution 1:1000, ab108319, Abcam), CX3CR1 (dilution 1:800, ab8012, Abcam), PSD95 (dilution 1:1000, ab18258, Abcam), NMDA2B (dilution 1:1000, ab65783, Abcam), β-tubulin (dilution 1:1000, sc-5274, Santa), β-actin (dilution 1:2000, sc-47778, Santa Cruz), and GAPDH (dilution 1:2000, E12-052, Enogene) overnight at 4°C. Signals were detected with HRP-conjugated goat, rabbit or mouse antibodies followed by chemiluminescence with a New Super ECL kit (KeyGEN BioTECH). 49 (link) Films were scanned and signals qualified by ImageJ (NIH, USA). Density of each band was normalized to controls.
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