Anaesthetized polyps (using 2% urethane for 2 min) were fixed using EM fixing solution [2.5% paraformaldehyde (Electron Microscopy Sciences, EMS), 2.5% glutaraldehyde (EMS) and 0.1 mol l−1 cacodylate buffer (Sigma)]. These polyps were then embedded in 5% agar and sectioned into 200 μm slices using a vibratome (Leica VT1200 S). The sections were then stained with 1% osmium tetroxide (Sigma), 1% tannic acid (EMS) and 1% uranyl acetate (EMS), and dehydrated by washing them serially with increasing concentrations of ethanol (30–100% ethanol). The sections were then dried at CO2 critical point using a critical point dryer (Leica CPD030), then mounted on carbon tapes and sputter-coated with platinum. The coated samples were imaged using the Sigma 500 electron microscope (Zeiss).
Sigma 500 electron microscope
Manufactured by Zeiss
Sourced in Germany
The Sigma 500 is an electron microscope manufactured by Zeiss. It provides high-resolution imaging of samples by using a focused beam of electrons to interact with the surface of the specimen. The Sigma 500 can be used to observe and analyze the microstructure and composition of a wide range of materials.
Automatically generated - may contain errors
Lab products found in correlation
Cacodylate buffer, by Merck Group (1 mentions)
Osmium tetroxide, by Merck Group (1 mentions)
Vt1200, by Leica (1 mentions)
Cpd 030, by Leica (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Paraformaldehyde (pfa), by Electron Microscopy Sciences (1 mentions)
Cf200 cu, by Electron Microscopy Sciences (1 mentions)
2 protocols using sigma 500 electron microscope
1
Ultrastructural Analysis of Anesthetized Polyps
2
PFF Characterization via Electron Microscopy
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Two μl of PFF diluted with 18 μl PBS and then pipet to mix. Then, PFF were treated onto a carbon-coated copper grid (#CF200-CU, Electron Microscopy Sciences, Ft. Washington, PA, USA) followed by negative staining with 2% uranyl acetate for 2 min. The grid was gently washed three times 1 min with distilled water. After drying the grid, it was observed by SIGMA500 electron microscope (Carl Zeiss, Oberkochen, Germany).
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