Physcomitrium patens subspecies patens (Hedwig) Mitten (Medina et al., 2019 ; Rensing et al., 2020 (link)) wild-type strains Gransden 2004, Gransden D12 and Villersexel, and previously published mutants were grown under sterile conditions on 42 mm Jiffy 7 peat pellets (Amazon, London). To produce data in Figures 2 –7 , pellets were first rehydrated using 40 ml of distilled water inside Magenta GA-7 culture vessels (Sigma-Aldrich, Gillingham, United Kingdom), sealed with Micropore tape (3 M, Maplewood, Minnesota, United States) and sterilized. Post-sterilization, a further 70 ml of sterilized distilled water was added. To produce sterile protonemal homogenate, a 1 × 9 cm plate of 6–10-day old BCDAT-grown tissue (Cove et al., 2009 (link)) was scraped from a cellophane disk (AA Packaging, Preston, United Kingdom) and placed in 15 ml of sterilized distilled water and homogenized for 20 s using a Polytron PT1200 (KINEMATICA AG, Luzern, Switzerland). Peat pellets were inoculated with either 1.5 ml of protonemal homogenate (Figures 2 –6 ) or a 1.5 cm2 piece of tissue derived from 3-week-old BCDAT-grown tissue (Figure 1 ). To produce the sporophytes presented in Figure 8 , plants were grown on agar plates (12 g l–1) supplemented with Knop medium (Egener et al., 2002 (link); Frank et al., 2005 (link)) as previously described Chater et al. (2016) (link).
Magenta ga 7 culture vessels
Manufactured by Merck Group
Sourced in United States
The Magenta GA-7 culture vessels are specialized laboratory equipment designed for tissue culture and plant growth applications. They provide a controlled environment for the cultivation of cells, tissues, or small plants. The vessels feature a transparent, polycarbonate construction and a built-in vent system to facilitate gas exchange. The core function of the Magenta GA-7 culture vessels is to provide a stable and sterile microenvironment for the cultivation and growth of biological samples.
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Lab products found in correlation
4 protocols using magenta ga 7 culture vessels
1
Protocols for Moss Physcomitrium patens
2
Optimizing Shoot Multiplication in Plant Tissue Culture
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Initiated shoots were cultured in Magenta GA-7 culture vessels (Sigma-Aldrich, St. Louis, Missouri, USA) containing 50-ml shoot multiplication medium. Shoot multiplication medium was MS medium containing different concentrations of BAP (0.5, 1.0, 1.5, 2.0, 2. 5 mg/l) in combination with Indole-3-butyric acid (IBA) (0.01, 0.1, 0.5 mg/l), TDZ (0.5, 1.0, 1.5, 2.0, 2. 5 mg/l) in combination with IBA (0.01, 0.1, 0.5 mg/l), or TDZ (0.5, 1.0, 1.5, 2.0, 2. 5 mg/l) in combination with α-Naphthalene acetic acid (NAA) (0.01, 0.1, 0.5 mg/l). Basal MS medium was used as control. The cultures were maintained at the same condition as for shoot initiation. For each treatment, a total of 30 explants were used. Number of shoots and shoot length were recorded after 4 weeks of culture. The whole experiment was repeated once.
3
Cladode Propagation via Tissue Culture
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Two types of explants were tested: Cladode segments and tips (3 cm length) were cultured in Magenta GA-7 culture vessels (77 × 77 × 97 mm; Sigma Chemical Co., St. Louis, MO, USA) that contained MS medium supplemented with 30 g L−1 of sucrose, 1 mg L−1 of BAP 6-benzylaminopurine and 0.1 mg L−1 of NAA naphthalene acetic acid and solidified with 0.8% agar agar. The pH of the medium was adjusted to 5.8 prior to autoclaving at 121 °C and 1.2 kg cm−2 pressure for 20 min. The environmental conditions of the growth chamber were adjusted to 23 °C ± 2 °C and 70 μmol m−2 s−1 PPFD for a 16 h photoperiod using cool white fluorescent lamps. The growth and cladode multiplication characteristics, i.e., the number of cladodes per explant, cladode length and fresh and dry weights, were compared for both explant types after 8 weeks in culture.
4
Establishing Sweet Potato Explant Cultures
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Explant collection, disinfection, and culture establishment Twigs (5-6 cm) of red-peeled Egyptian sweet potato (Ipomoea batatas [L.] Lam.) 'Abees' were excised from the actively growing shoots of stock plants grown and maintained at a local farm in Kafr El Sheikh Governorate, Egypt. The twigs were cleaned under running tap water for 10 min and rinsed three times using sterile distilled water. Then, twigs were disinfected by submerging them in 70% (v/v) ethanol for 20 s and then transferring them to a 0.1% (w/v) mercuric chloride solution that contained 2-3 drops of Tween 20 for 5 min. Finally, twigs were rinsed three more times using sterile distilled water, and 0.5 to 1 cm twig segments (i.e., nodal explants) were cultured in glass test tubes (24 × 200 mm) that contained 15 mL basal Murashige-Skoog (MS) medium (Murashige and Skoog, 1962) , which had been supplemented with 3% (w/v) sucrose and 0.8% (w/v) agar-agar plugged with plastic caps. In vitro regenerated shoots (Figure 1A) were cut into nodal segments (1 cm, with one axillary bud), cultured in Magenta GA-7 culture vessels (77 ×77× 97 mm; Sigma Chemical, St. Louis, Missouri, USA) that contained semisolid MS medium without plant growth regulators (PGRs). The regenerated shoots were then subcultured on the same medium every 4 wk until enough plant material was available to conduct the experiments.
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