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Gfp 11 814 460 001

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The GFP (11 814 460 001) is a lab equipment product from Roche. It is a fluorescent protein that can be used as a marker in biological research.

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Lab products found in correlation

Ku55933, by R&D Systems (2 mentions) Mnase, by Merck Group (2 mentions) Cpd tdm 2, by MBL Life Science (2 mentions) Dna modifying enzymes, by Roche (2 mentions) Nu7441, by R&D Systems (2 mentions) Rnaseh2a nbp1 76981, by Novus Biologicals (2 mentions) Snrnp40 sab2701506 and srsf2 sc35 clone sc 35, by Merck Group (2 mentions) Snrpa u1a 3f9 1f7, by Abcepta (2 mentions) Phospho chk2 thr68 2661, by Cell Signaling Technology (2 mentions) Odyssey compatible irdye680 and irdye800 conjugated secondary antibodies, by LI COR (2 mentions) β tubulin, by Santa Cruz Biotechnology (2 mentions) Gfp trap beads, by Proteintech (2 mentions) Pladienolide b, by Santa Cruz Biotechnology (2 mentions) Anti tubulin, by Bio-Rad (1 mentions) Sb431542, by Merck Group (1 mentions) Fk506, by Merck Group (1 mentions) F7425, by Merck Group (1 mentions) Phospho smad3, by Cell Signaling Technology (1 mentions) Total stat3, by Cell Signaling Technology (1 mentions) Smad2 3, by BD (1 mentions)

4 protocols using gfp 11 814 460 001

1

Comprehensive Chromatin Immunoprecipitation and Protein Interaction Mapping

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MNase and all chemicals were purchased from Sigma-Aldrich unless otherwise specified. DNA modifying enzymes were from Roche Applied Sciences. Pladienolide B was from Santa Cruz Biotechnology, the ATR inhibitor VE821 from TINIB-Tools, and the ATM inhibitor KU55933 and DNA-PK inhibitor NU7441 from R&D Systems. Antibodies used were against: PRP8 (H300), XPA/p62 (FL-273), p89/XPB (S-19) and β-Tubulin from Santa Cruz Biotechnology; SNRPC/U1C (NBP1-96048), NHP2L1 (NBP1-32732), SF3a1 (NB100-79847), SF3b2 (NV100-79843), RNaseH1 (NBP2-20171), and RNaseH2A (NBP1-76981) from Novus Biologicals; SNRNP40 (SAB2701506) and SRSF2/SC35 (clone SC-35) from Sigma; SNRPA/U1A (3F9-1F7) from ABGENT; PRPF3 (ab187535), RNPII CTD (phospho-S2) (ab5095), RNAPII (ab5095), PCNA (PC-10), Ki67 (ab833) from abcam®; CPD (TDM-2) from MBL International; GFP (11 814 460 001) from Roche; H2A (07-146) from Millipore Corp.; phosphor-ATM(1981)(05-740) from Upstate Biotechnology, Phospho-CHK2(Thr68) (2661) from Cell Signaling. Anti-XPC (rabbit-polyclonal ab) was in-house developed. Odyssey-compatible IRDye680- and IRDye800–conjugated secondary antibodies were from LI-COR. Secondary antibodies conjugated to Alexa Fluorochromes-488, -568, -594 and-647 were from Invitrogen. GFP-tagged proteins were immunoprecipitated with GFP-Trap® beads (ChromoTek).
Tresini M., Warmerdam D.O., Kolovos P., Snijder L., Vrouwe M.G., Demmers J.A., van IJcken W.F., Grosveld F.G., Medema R.H., Hoeijmakers J.H., Mullenders L.H., Vermeulen W, & Marteijn J.A. (2015). The core spliceosome as target and effector of non-canonical ATM signaling. Nature, 523(7558), 53-58.
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2

Antibody-Based Protein Expression Analysis

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Antibodies: Phospho-Smad2 (#3108, Cell Signaling Tech. 1/1000), phospho-Smad3(#9520, Cell Signaling Tech. 1/1000), phospho-Stat3 (#9131, Cell Signaling Tech. 1/1000), phospho-Jak2 (#3771, Cell Signaling Tech. 1/1000), Snail (#3879, Cell Signaling Tech. 1/1000), EEA1 (#C45B10, Cell Signaling Tech. 1/100) and total Stat3(#4904, Cell Signaling Tech. 1/1000) were purchased from Cell Signaling Technology; SMAD2/3 (610843, BD Biosciences, 1/1000), anti-myc (sc789, 1/1000 and 9E10, sc40, 1/1000), TβRI (sc398, 1/500) Lamin B (sc6217, 1/1000) TβRII (sc17792, 1/500), FKBP12 (sc6174, 1/500), GAPDH (sc32233, 1/1000) and GPR50 (sc50590, 1/500) came from Santa Cruz Biotechnology. Monoclonal and polyclonal Flag-Antibodies were used from Sigma-Aldrich (M2-F3165, 1/1000, and F7425, 1/1000). HA (11867423001, 1/1000) and GFP (11814460001, 1/2000) antibodies were used from Roche. Anti-Tubulin was purchased from AbD Serotec (MCA77G, 1/2000). GPR50 antibody7 was produced by Kernov Antibody Services (1/1000)49 (link). Polyclonal Anti GPR50 (H00009248-B01P, 1/500) was purchased from Novus Biologicals. Anti TGFβRI-pS165 (ab112095, 1/500) was purchased from Abcam. All antibodies were employed according to the recommended dilutions for either immunoprecipitation or western blotting.
Reagents: FK506 and SB431542 were purchased from Sigma Aldrich and recombinant TGFβ-1 was purchased from Peprotech.
Wojciech S., Ahmad R., Belaid-Choucair Z., Journé A.S., Gallet S., Dam J., Daulat A., Ndiaye-Lobry D., Lahuna O., Karamitri A., Guillaume J.L., Do Cruzeiro M., Guillonneau F., Saade A., Clément N., Courivaud T., Kaabi N., Tadagaki K., Delagrange P., Prévot V., Hermine O., Prunier C, & Jockers R. (2018). The orphan GPR50 receptor promotes constitutive TGFβ receptor signaling and protects against cancer development. Nature Communications, 9, 1216.
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3

DYRK1B Antibody Production and Validation

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DYRK1B antibodies produced in rabbits using the immunogen CGLRGVPQSTAASS for the Western blot antibody and MAVPPGHGPFSGC for the antibody used for immunoprecipitation assays were provided by AstraZeneca (Alderley Park, Macclesfield, UK) and were described previously. The p-S421-DYRK1B antibody, also from AstraZeneca, was produced in rabbits immunised with the BSA conjugated phospho-peptide ac-CGEPAARI(P)SPLGALQHG-nh2, prepared using standard procedures [37 ]. Antibodies specific for p-T202/Y204-ERK1/2 (9106), ERK1/2 (4695), p-T180/Y182-p38 (9211), p-T183/Y185-JNK (9251), pT286-cyclin D1 (2921), p-S33/S37/T41-β Catenin (9561) were from Cell Signaling Technologies; ERK1 (610031), HSP90 (610418) and β-Catenin (610153) were from BD Biosciences, β-actin (A5441) was from Sigma; GFP (11814460001) was from Roche; cyclin D1 (CC12) and Ras (OP40) were from Calbiochem. JNK1/3 (sc474) and ERK2 (sc154) were from Santa Cruz Biotechnology; Flag (MAB3118) was from Millipore; p38 antibody was provided by the Babraham Institute Monoclonal Antibody Facility.
Ashford A.L., Dunkley T.P., Cockerill M., Rowlinson R.A., Baak L.M., Gallo R., Balmanno K., Goodwin L.M., Ward R.A., Lochhead P.A., Guichard S., Hudson K, & Cook S.J. (2015). Identification of DYRK1B as a substrate of ERK1/2 and characterisation of the kinase activity of DYRK1B mutants from cancer and metabolic syndrome. Cellular and Molecular Life Sciences, 73, 883-900.
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4

Comprehensive Chromatin Immunoprecipitation and Protein Interaction Mapping

Check if the same lab product or an alternative is used in the 5 most similar protocols
MNase and all chemicals were purchased from Sigma-Aldrich unless otherwise specified. DNA modifying enzymes were from Roche Applied Sciences. Pladienolide B was from Santa Cruz Biotechnology, the ATR inhibitor VE821 from TINIB-Tools, and the ATM inhibitor KU55933 and DNA-PK inhibitor NU7441 from R&D Systems. Antibodies used were against: PRP8 (H300), XPA/p62 (FL-273), p89/XPB (S-19) and β-Tubulin from Santa Cruz Biotechnology; SNRPC/U1C (NBP1-96048), NHP2L1 (NBP1-32732), SF3a1 (NB100-79847), SF3b2 (NV100-79843), RNaseH1 (NBP2-20171), and RNaseH2A (NBP1-76981) from Novus Biologicals; SNRNP40 (SAB2701506) and SRSF2/SC35 (clone SC-35) from Sigma; SNRPA/U1A (3F9-1F7) from ABGENT; PRPF3 (ab187535), RNPII CTD (phospho-S2) (ab5095), RNAPII (ab5095), PCNA (PC-10), Ki67 (ab833) from abcam®; CPD (TDM-2) from MBL International; GFP (11 814 460 001) from Roche; H2A (07-146) from Millipore Corp.; phosphor-ATM(1981)(05-740) from Upstate Biotechnology, Phospho-CHK2(Thr68) (2661) from Cell Signaling. Anti-XPC (rabbit-polyclonal ab) was in-house developed. Odyssey-compatible IRDye680- and IRDye800–conjugated secondary antibodies were from LI-COR. Secondary antibodies conjugated to Alexa Fluorochromes-488, -568, -594 and-647 were from Invitrogen. GFP-tagged proteins were immunoprecipitated with GFP-Trap® beads (ChromoTek).
Tresini M., Warmerdam D.O., Kolovos P., Snijder L., Vrouwe M.G., Demmers J.A., van IJcken W.F., Grosveld F.G., Medema R.H., Hoeijmakers J.H., Mullenders L.H., Vermeulen W, & Marteijn J.A. (2015). The core spliceosome as target and effector of non-canonical ATM signaling. Nature, 523(7558), 53-58.
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