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Sodium iodide nai

Manufactured by Merck Group
Sourced in United States

Sodium iodide (NaI) is a chemical compound used in various laboratory applications. It is a crystalline solid with a high refractive index and is commonly used as a scintillator material in radiation detection equipment, such as gamma-ray spectrometers and X-ray detectors. Sodium iodide is known for its ability to efficiently convert high-energy radiation into visible light, which can then be detected and measured by photomultiplier tubes or other light-sensing devices.

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7 protocols using sodium iodide nai

1

Peptide Synthesis and Purification Protocol

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SJMHE1 437-460 (VPGGGTALLRCIPVLDTLSTKNED) was synthesized and purified by Top-peptide (Shanghai, China) as previously described 18 (link). FSL-1 was purchased from MedChem Express (Monmouth Junction, NJ); Sodium iodide (NaI) was purchased from Sigma-Aldrich (St. Louis, MO); anti-TLR2, anti-TLR4, and IgG isotype control antibodies were purchased from BioLegend (San Diego, CA).
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2

In-vitro and In-vivo NIS Expression

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Hela cells were obtained from ATCC, cultured in 4.5 g/l Dulbecco’s modified Eagle’s medium supplemented with 10% (v/v) of fetal bovine serum and with penicillin and streptomycin and maintained in a humidified atmosphere of 5% CO2 at 37°C. Cumate (4-isopropylbenzoic acid), sodium perchlorate (NaCIO4) and sodium iodide (NaI) were purchased from Sigma-Aldrich (Sigma-Aldrich, USA). The Lipofectamine RNAiMAX transfection reagent, synthetic microRNA mimics and RIPA lysing buffer were from Thermofisher (Thermofisher, USA). The Pluronic F-68 formulation used to transfect the tibialis anterior muscle in vivo was also purchased from Sigma Aldrich. The human specific NIS antibody (clone 17795) was from Abcam (Abcam, USA), the APAF-1 (clone D5C3), Caspase 9 (clone 353) antibodies were from Cell signalling Technologies (Cell signalling Technologies, USA) and the tubulin (clone TU-02) antibody was from Santa Cruz Biotechnology. The luciferin substrate for in vivo use was purchased from Promega (Promega, USA). The 99mTcO4 pertechnetate (99mTcO4-) was from IBA Molecular (900 MBq, IBA Molecular, France) and was collected freshly from the Nuclear medicine department of a neighboring hospital (Centre Hospitalier Regional d’Orléans, Orléans, France) and used within the day.
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3

Dye-Sensitized Solar Cell Fabrication

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Activated carbon (AC, P4) was provided by SGL Carbon (Germany). Sodium carboxymethyl cellulose (CMC, Walocell CRT 2000) was purchased from Dow Wolff Cellulosics. Conductive carbon (CC, Super C65) was purchased from Imerys Graphite & Carbon. The ionic liquid Pyr14TFSI (99.9%, Solvionic) was dried under high vacuum (10−7 mbar) for 24 h at 120°C. Its water content after drying, determined by Karl Fischer titration (Mettler Toledo), was below 10 ppm. Benzophenone (for synthesis, ≥99.0%, Merck) was dried under vacuum for 48 h at 40°C. Poly(ethylene oxide) (PEO, Mv = 100,000, Dow Chemical) was dried under vacuum for 48 h at 50°C. The sensitizing dye, cis-bis(isothiocyanato)(2,2'-bipyridyl-4,4'-dicarboxylato)(4,4'-di-nonyl-2'bipyridyl) ruthenium (II) (Z907, Ruthenizer 520-DN) was purchased from Solaronix. TiO2 Paste DSL 18NR-AO was purchased from Dyesol. The Meltonix 1170-60 (60 μm) sealant was purchased from Solaronix. Solar cells electrolyte components, sodium iodide (NaI) and iodine (I2) were purchased from Sigma-Aldrich (Milan, Italy). 4-tert-butylpyridine (TBP) and methoxypropionitrile (CH3OCH2CH2CN) were purchased from Merck. 7 Ω sq−1 sheet resistance fluorine-doped tin oxide (FTO)-coated glass was purchased by Solaronix.
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4

Investigating Inflammatory Signaling Pathways

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Primary antibodies, such as rabbit anti-NFκB p65, rabbit anti-p-NFκB p65 (Ser536), and rabbit anti-β-actin antibodies, were obtained from Cell Signaling Technology (USA). Anti-GSDMDC1 (64-Y), anti-IL-1β, anti-Hsp60, HRP-labeled goat anti-rabbit IgG, and HRP-labeled goat anti-mouse IgG were purchased from Santa Cruz Biotechnology (USA). The anti-NLRP3 antibody was acquired from Abcam (Cambridge, UK). The IL-1β Quantikine ELISA kit was from MultiSciences (China). The ROS assay kit, N-acetyl-cysteine (NAC) and Cell Counting Kit-8 (CCK-8) were from Beyotime Biotech (China). IKK-16 was purchased from Selleck Chemicals (USA). Sodium iodide (NaI) was purchased from Sigma-Aldrich.
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5

Inflammation Model of Thyroid Cells

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The normal human thyroid epithelial cell line Nthy-ori 3-1 was given generously by the First Affiliated Hospital of China Medical University, China. Cells were cultured in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA) containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and 1% penicillin–streptomycin, incubated at 37°C with 5% carbon dioxide. The inflammation model of Nthy-ori 3-1 was constructed with the stimulation of TNF-α (20 ng) (Sigma, St. Louis, MO, USA) and varied doses of sodium iodide (NaI; Sigma) and co-stimulated with JSK-J4 (20 µmol; Selleck, Houston, TX, USA) for 6 and 24 h.
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6

Iodide Challenge in Nrf2 Mice

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C57BL/6J Nrf2+/− mice, obtained from RIKEN BRC (Tsukuba, Japan) [22 (link)], were used to generate wild-type (WT) and Nrf2 knockout mice (KO). For iodide challenge, male WT and KO mice (three to four months old) fed a standard diet (product number 4RF21, Mucedola, Italy, containing 1 mg/kg iodine) were supplied with normal tap water with or without 0.05% sodium iodide (NaI) (Sigma, St Louis, MO, USA) for seven days. Thus, the study comprises the following four groups of mice: WT mice on regular tap water (WT control (WTC), n = 9), WT mice on 0.05% NaI (WT on iodide (WTI), n = 12), KO mice on regular tap water (KO control, (KOC), n = 10), and KO mice on 0.05% NaI (KO on iodide (KOI), n = 10). Mice were housed in the animal facility of the University of Patras Medical School in temperature-, light-, and humidity-controlled rooms with a 12 h light/dark cycle. All animal procedures were approved by the institutional review board of the University of Patras Medical School (approval ID 15/2008) and were in accordance with EC Directive 86/609/EEC.
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7

Synthesis and Characterization of Cyclodextrin Derivatives

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EDOT was purchased from Sigma-Aldrich and purified before use by vacuum distillation. Sodium hydride (NaH) 60% dispersion in mineral oil and iodomethane were acquired from Sigma-Aldrich. β-CD and γ-CD were purchased from CycloLab Ltd. (Budapest, Hungary), recrystallized twice from water, and then dried in vacuum at 95 °C for 24 h prior to use. For MALDI MS analysis, the matrix (trans-2-(3-(4-tert-butylphenyl)-2-methyl-2-propenylidene)) malononitrile-DCTB and sodium iodide (NaI) were purchased from Sigma Aldrich (Saint Louis, MO, USA). Ultrapure water and all other solvents were purchased from commercial sources (Sigma-Aldrich, Fischer (Ried im Innkreis, Austria)) and used as received.
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