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Recombinant rat pedf

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Recombinant rat PEDF is a laboratory product produced in a recombinant system. It is a protein that has the core function of pigment epithelium-derived factor.

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Lab products found in correlation

Mpo colorimetric activity assay kit, by Merck Group (1 mentions) Bca protein concentration determination kit, by Beyotime (1 mentions) Dapi staining solution, by Keygen Biotech (1 mentions) Ldh cytotoxicity assay kit, by Beyotime (1 mentions) Annexin 5 fitc pi apoptosis detection kit, by Keygen Biotech (1 mentions) Tunel kit, by Keygen Biotech (1 mentions) Lipofectamine 3000, by GE Healthcare (1 mentions) Pgex 6p 1, by GE Healthcare (1 mentions) Human embryonic kidney 293t cells, by American Type Culture Collection (1 mentions) Pparg, by Abcam (1 mentions) 2 7 dichlorofluorescin diacetate dcfh da, by Merck Group (1 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (1 mentions) Atglistatin, by Merck Group (1 mentions) Annexin 5 fitc pi staining kit, by Roche (1 mentions) Anti p akt antibody, by Cell Signaling Technology (1 mentions) Cell counting kit 8, by Dojindo Laboratories (1 mentions) Anti β actin, by Cell Signaling Technology (1 mentions) Hoechst 33342, by Merck Group (1 mentions) Necrostatin 1, by Merck Group (1 mentions)

Close spelling variants of this product

Recombinant PEDF

8 protocols using recombinant rat pedf

1

Recombinant Rat PEDF for Cardiomyocyte Protection

Cited 2 times
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Recombinant rat PEDF (GenBank™ accession no. NM_177927) was synthesized by Cusabio Biotech, Co., Ltd. (Wuhan, China) as described previously (20 (link)). Our previous studies demonstrated that 10 nmol/l is the optimal PEDF concentration for cardiomyocyte protection (21 (link)).
Huang B., Miao H., Yuan Y., Qiu F., Liu X., Liu Z., Zhang H., Zhao Q., Wang M., Dong H, & Zhang Z. (2019). PEDF decreases cardiomyocyte edema during oxygen-glucose deprivation and recovery via inhibiting lactate accumulation and expression of AQP1. International Journal of Molecular Medicine, 43(5), 1979-1990.
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2

Investigating PEDF and Inflammatory Pathways

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Recombinant rat PEDF (GenBank accession number: NM_177927): Cusabio Biotech Co, Ltd (Wuhan, Hubei, China); The bacterial lipopolysaccharide (LPS) (L4391, O111:B4) and MPO colorimetric activity assay kits: Sigma (St. Louis, MO, USA). The LDH cytotoxicity assay kit and BCA protein concentration determination kit: Beyotime (Shanghai, China); Tissue and cell total protein extraction kits: Sangon Biotech (Shanghai, China). ELISA kits to measure TNF-α, IL-1β, and IL-6: Shanghai Renjie Biotechnology Co., Ltd. (Shanghai, China) [15 (link)]. ELISA kits to measure PEDF: Shanghai Yan Qi Biological Technology Co Ltd. The TUNEL kit, annexin V‑FITC/PI apoptosis detection kit, and DAPI staining solution: KeyGEN Biotech (Nanjing, Jiangsu, China). Anti-rabbit cleaved-caspase-3 (Catalog No. #9661), anti-rabbit p38 MAPK (Catalog No. #8690), anti-rabbit phospho-p38 MAPK (Catalog No. #4511), anti-rabbit NF-κB p65 (Catalog No. #8242), and anti-rabbit phospho-NF-κB p65 (Catalog No. #3033) antibodies: Cell Signaling Technology (Danvers, MA, USA). Anti-rabbit NLRP3 (Catalog No. ab263899), anti-rabbit PPAR-γ (Catalog No. ab178860) antibodies and PPAR-γ inhibitor (GW9662, Catalog No. ab141125): Abcam (Cambridge, MA, USA) [16 (link)]. Anti-rabbit RIP3 (Catalog No. 17563–1-AP) and anti-mouse β-tubulin (Catalog No. 66240–1-Ig) antibodies: Proteintech (Wuhan, Hubei, China).
Xu L., Chen Y., Feng S., Liu Z., Ye Y., Zhou R, & Liu L. (2023). PEDF inhibits LPS-induced acute lung injury in rats and promotes lung epithelial cell survival by upregulating PPAR-γ. BMC Pulmonary Medicine, 23, 359.
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3

Recombinant Rat PEDF Synthesis

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Recombinant rat PEDF (GenBank™ accession no. NM_177927) was synthesized by Cusabio Biotech, Co., Ltd. (Wuhan, China).
Zhang Y., Li Y., Liu Z., Zhao Q., Zhang H., Wang X., Lu P., Yu H., Wang M., Dong H, & Zhang Z. (2018). PEDF regulates lipid metabolism and reduces apoptosis in hypoxic H9c2 cells by inducing autophagy related 5-mediated autophagy via PEDF-R. Molecular Medicine Reports, 17(5), 7170-7176.
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4

Recombinant PEDF Protein Purification

Cited 1 time
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Recombinant rat PEDF (GenBank accession no. NM_177927) was synthesized by Cusabio Biotech, Co., Ltd. (Wuhan, China). In brief, 293T cells were transfected with the 20 μg recombinant vector pGEX 6P-1 (GE Healthcare, Chicago, IL, USA), containing glutathione S-transferase (GST)-tagged PEDF using Lipofectamine® 3000 according to the manufacturer's protocols. GST-tagged PEDF proteins were purified by high-pressure liquid chromatography purification (>90% purity) and amino-terminal sequence determination (23 ,24 (link)). The resultant proteins were soluble in aqueous solutions.
Zhao Q., Liu Z., Huang B., Yuan Y., Liu X., Zhang H., Qiu F., Zhang Y., Li Y., Miao H., Dong H, & Zhang Z. (2018). PEDF improves cardiac function in rats subjected to myocardial ischemia/reperfusion injury by inhibiting ROS generation via PEDF-R. International Journal of Molecular Medicine, 41(6), 3243-3252.
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5

Recombinant Rat PEDF Purification

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Recombinant rat PEDF (GenBankTM accession number: NM_177927) was synthesized by Cusabio Biotech, Co., Ltd. (Wuhan, China). In brief, 293T cells were transfected with the recombinant vector pGEX 6P‐1 glutathione S‐transferase (GST)‐tagged PEDF. GST‐tagged PEDF proteins were purified by high‐pressure liquid chromatography purification (>90% purity) and amino‐terminal sequence determination. The resulting proteins were soluble in aqueous solutions.
Lu P., Zhang Y., Zhang H., Li Y., Wang X., Xu H., Liu Z., Li L., Dong H, & Zhang Z. (2016). Pigment Epithelium‐Derived Factor (PEDF) Improves Ischemic Cardiac Functional Reserve Through Decreasing Hypoxic Cardiomyocyte Contractility Through PEDF Receptor (PEDF‐R). Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 5(7), e003179.
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6

Recombinant Rat PEDF Protein Purification

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Recombinant rat PEDF (GenBank accession number: NM_177927) was synthesized by Cusabio Biotech, Co, Ltd (Wuhan, China). In brief, 293T human embryonic kidney cells (American Type Culture Collection, Manassas, VA) were transfected with the recombinant vector pGEX 6P‐1 glutathione S‐transferase–tagged PEDF. Glutathione S‐transferase–tagged PEDF proteins were purified by high‐pressure liquid chromatography (>90% purity) and amino‐terminal sequence determination. The resulting proteins were soluble in aqueous solution.
Liu X., Liu Z., Chen J., Zhu L., Zhang H., Quan X., Yuan Y., Miao H., Huang B., Dong H, & Zhang Z. (2019). Pigment Epithelium–Derived Factor Increases Native Collateral Blood Flow to Improve Cardiac Function and Induce Ventricular Remodeling After Acute Myocardial Infarction. Journal of the American Heart Association: Cardiovascular and Cerebrovascular Disease, 8(22), e013323.
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7

Recombinant Rat PEDF Protein Synthesis

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Recombinant rat PEDF (GenBankTM Accession Number NM_177927) was synthesized by Cusabio Biotech, Co., Ltd. (Wuhan, China). In brief, 293T cells were transfected with the recombinant vector pGEX 6P-1 GST-tagged PEDF. GST-tagged PEDF proteins were purified by high pressure liquid chromatography purification (>90% purity) and amino-terminal sequence determination. The resulting proteins were soluble in aqueous solutions.
Zhang H., Hui H., Li Z., Pan J., Jiang X., Wei T., Cui H., Li L., Yuan X., Sun T., Liu Z., Zhang Z, & Dong H. (2017). Pigment epithelium-derived factor attenuates myocardial fibrosis via inhibiting Endothelial-to-Mesenchymal Transition in rats with acute myocardial infarction. Scientific Reports, 7, 41932.
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8

Evaluation of Cell Death Pathways

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Anti-p-Akt antibody (#0458), anti-cleaved caspase-3 (#9664), and anti-β-actin (#13E5) antibodies were purchased from Cell Signaling Technology, USA. PPARg (#ab70405) was purchased from Abcam, Inc. (Abcam, Cambridge,UK). Annexin V-FITC/PI Staining Kit was purchased from Roche, Switzerland. Atglistatin (#SML1075), methyl methacrylate monomer (#55909), necrostatin-1 (#N9037), Hoechst33342 (#861405) and 2`,7`-dichlorofluorescin diacetate (DCFH-DA;#D6883) were purchased from Sigma, USA. Superoxide dismutase (SOD; #A001-1) and malonaldehyde (MDA; #A002-1) detection kit were purchased from JIANCHENG Bioengineering Institute, China. Recombinant rat PEDF was synthesized by CUSABIO BIOTECH CO. Ltd, China. Cell Counting Kit (CCK-8) was from Dojindo Molecular Technologies (Kumamoto, Japan).
Xin L., Han T., Tang J., Wang X., Zhang H., Dong H., Guo K, & Zhang Z. (2017). Pigment epithelium derived factor (PEDF) prevents methyl methacrylate monomer-induced cytotoxicity in H9c2 cells. Journal of Biomedical Research, 31(6), 512-520.
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