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Apc cd151

Manufactured by BioLegend

APC-CD151 is a fluorochrome-conjugated monoclonal antibody that specifically binds to the CD151 antigen. CD151 is a cell surface glycoprotein that belongs to the tetraspanin family of proteins. The APC-CD151 antibody can be used for the identification and characterization of CD151-expressing cells by flow cytometry.

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2 protocols using apc cd151

1

Immune Cell Subset Characterization

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Excised tissues were prepared as previously described (41 (link)). Cell suspensions were blocked by the addition of Gamunex (Baxter, Deerfield, IL) for 30 minutes at RT, then washed in flow buffer (PBS + 2%FBS) and incubated at RT for 1 hour with the following antibodies (at a 1:10 final concentration): PerCP/Cy5.5-CD3, APC-CD34, PE-CD45, and/or APC-CD151 (BioLegend). Flow analysis was conducted on a Beckman Coulter Cyan. Cell sorting was performed on the Beckman Coulter Moflo XDP 70. Data analysis was performed using Summit, V5.1 (Beckman Coulter, Brea, CA).
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2

Immune Cell Subset Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Excised tissues were prepared as previously described (41 (link)). Cell suspensions were blocked by the addition of Gamunex (Baxter, Deerfield, IL) for 30 minutes at RT, then washed in flow buffer (PBS + 2%FBS) and incubated at RT for 1 hour with the following antibodies (at a 1:10 final concentration): PerCP/Cy5.5-CD3, APC-CD34, PE-CD45, and/or APC-CD151 (BioLegend). Flow analysis was conducted on a Beckman Coulter Cyan. Cell sorting was performed on the Beckman Coulter Moflo XDP 70. Data analysis was performed using Summit, V5.1 (Beckman Coulter, Brea, CA).
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