β catenin antibody

Manufactured by Abmart

The β-catenin antibody is a laboratory reagent used to detect and study the β-catenin protein. β-catenin is a key component of the Wnt signaling pathway and plays a role in cell-cell adhesion. The antibody can be used in various analytical techniques, such as Western blotting and immunohistochemistry, to investigate the expression and localization of β-catenin in biological samples.

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Lab products found in correlation

Hrp conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) β actin antibody, by Bioworld Technology (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Primescript rt master mix, by Takara Bio (1 mentions) Sybr green pcr master mix, by Takara Bio (1 mentions) Trizol, by Takara Bio (1 mentions) β catenin antibody, by Abcam (1 mentions) Anti ub antibody, by Abcam (1 mentions) Mg132, by Abcam (1 mentions) Protein a g beads, by Abmart (1 mentions)

2 protocols using β catenin antibody

Comprehensive Molecular Analysis Workflow

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The medium and fetal bovine serum (FBS) were purchased from Invitrogen (Carlsbad, CA). Trizol, PrimeScript RT Master Mix and SYBR green PCR master mix were from Takara (Shiga, Japan). GRP78 and uPA antibodies were from Abcam (Cambridge, UK). β-actin antibody was from Bioworld Technology (Minneapolis, MN). GAPDH and GFP antibodies were from Sangon Biotech (Shanghai, China). β-catenin antibody was obtained from Abmart (Shanghai, China). FITC-, TRITC- and HRP-conjugated secondary antibodies were obtained from Invitrogen (Carlsbad, CA).

Li Z., Zhang L., Li H., Shan S, & Li Z. (2014). Glucose regulated protein 78 promotes cell invasion via regulation of uPA production and secretion in colon cancer cells. BMB Reports, 47(8), 445-450.

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Evaluating Protein Ubiquitination and Interaction

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Immunoprecipitation and ubiquitination evaluation were performed as previously described^{32 (link)}. In brief, cells were treated with MG132 (Abcam) at a final concentration of 20 μm for 4 h before collection. Whole-cell lysates were prepared in RIPA buffer and precipitated using protein A/G beads (Abmart) with β-Catenin antibody (Abmart). Precipitated products were assayed by immunoblot (IB) analysis with anti-Ub antibody (Abcam) or β-Catenin antibody.

Zhang B., Li Z., Zhang R., Hu Y., Jiang Y., Cao T., Wang J., Gong L., Ji L., Mu H., Yang X., Dai Y., Jiang C., Yin Y, & Zou J. (2019). PKCγ promotes axonal remodeling in the cortico-spinal tract via GSK3β/β-catenin signaling after traumatic brain injury. Scientific Reports, 9, 17078.

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