Sc 7345

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Sc-7345 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is designed for general laboratory use. The core function of this product is to provide a reliable and efficient tool for various scientific applications.

Automatically generated - may contain errors

Lab products found in correlation

Sc 6254, by Santa Cruz Biotechnology (2 mentions) Magna rip rna binding protein immunoprecipitation kit, by Merck Group (1 mentions) Ab190475, by Abcam (1 mentions) Ab76310, by Abcam (1 mentions) Sc 373841, by Santa Cruz Biotechnology (1 mentions) Triton x 100 x100, by Merck Group (1 mentions) Sc 5298, by Santa Cruz Biotechnology (1 mentions) Anti gapdh, by Santa Cruz Biotechnology (1 mentions) Sc 11418, by Santa Cruz Biotechnology (1 mentions) Ab34710, by Abcam (1 mentions) Anti p p38, by Cell Signaling Technology (1 mentions) Anti p38, by Cell Signaling Technology (1 mentions) Ab137474, by Abcam (1 mentions) Ab53096, by Abcam (1 mentions) Ab12169, by Abcam (1 mentions) Sc 14939, by Santa Cruz Biotechnology (1 mentions) Sc 271269, by Santa Cruz Biotechnology (1 mentions) Sc 146, by Santa Cruz Biotechnology (1 mentions) Anti smad4, by Cell Signaling Technology (1 mentions) Anti hdac4, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

JNK (sc-7345) (5 citations) Rabbit anti‐JNK (sc‐7345 (2 citations) Mouse anti‐JNK (sc‐7345) mouse anti‐p‐JNK (sc‐6254) (2 citations)

6 protocols using sc 7345

RNA-binding protein immunoprecipitation

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Magna RIP™ RNA-binding protein immunoprecipitation kit (Millipore) was applied for RIP assays. Specifically, the 1 × 10⁷ BV2 cell lysate was incubated with the negative control anti-IgG (ab190475, Abcam), anti-JNK (sc-7345, 1:500, Santa Cruz Biotechnology), anti-DUSP1 (sc-373841, 1:500, Santa Cruz Biotechnology), and anti-DUSP6 (ab76310, 1:1000, Abcam) conjugated magnetic beads. Subsequently, the RNA in the immunoprecipitation was extracted and the expression of TCONS_00145741 was analyzed using qRT-PCR.

Wu L., Zhan Q., Liu P., Zheng H., Liu M., Min J., Xie L, & Wu W. (2022). LncRNA TCONS_00145741 Knockdown Prevents Thrombin-Induced M1 Differentiation of Microglia in Intracerebral Hemorrhage by Enhancing the Interaction Between DUSP6 and JNK. Frontiers in Cell and Developmental Biology, 9, 684842.

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Isoliquiritigenin Platelet Activation Assay

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Isoliquiritigenin (961-29-5; the structure is listed in Figure 1A) was purchased from Weikeqi-biotech. Thrombin (T4393), ADP (A5285), PGE₁ (P7527), fibrinogen (F3879), TRITC-Phalloidin (P1951), dimethyl sulfoxide (DMSO; D2650), bovine serum albumin (BSA; B2064), and Triton X-100 (X100) were purchased from Sigma-Aldrich. Collagen (LS001652) was purchased from Chrono-Log. Fluo 3-AM (F023-10) was purchased from Dojindo. Fluorescein-conjugated antibody to human CD62P (Clone: AK-4) and PAC-1 (Clone: PAC-1) were purchased from BD Biosciences. The antibodies for the immunoblot in this study were the following: anti-phospho-PLCγ2 (Tyr759) antibody (50535, CST), anti-phospho-ERK1/2 (Thr202/Tyr204) antibody (4370, CST), anti-ERK1/2 antibody (9194, CST), anti-phospho-Jun N-terminal kinase (JNK) (Thr183/Tyr185) antibody (sc-6254, Santa Cruz), anti-JNK antibody (sc-7345, Santa Cruz), anti-phospho-p38 MAPK (Thr180/Tyr182) antibody (4511, CST), anti-p38 MAPK antibody (8690, CST), anti-phospho-Akt (Ser473) antibody (4060, CST), anti-Akt antibody (sc-5298, Santa Cruz), and anti-GAPDH (ANT011, Antegene).

Wu Y., Deng C., Wang Y., Ming Z., Mei H, & Hu Y. (2023). The inhibitory effect of isoliquiritigenin on human platelets in vitro. Annals of Translational Medicine, 11(6), 250.

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Piceatannol Inhibits Fibrosis Markers

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Piceatannol was purchased from Future Chem (Seoul, Korea). Anti-alpha smooth muscle actin (α-SMA; 1:1000, sc-130617), anti-CTGF (1:1000, sc-14939), anti-HDAC3 (1:1000, sc-11417), anti-HDAC4 (1:1000, sc-11418), anti-HDAC5 (1:1000, sc-133225), anti-TGF-β1 (1:1000, sc-146), anti-JNK (1:1000, sc-7345), anti-ERK1 (1:1000, sc-271269), and anti-GAPDH (1:1000, sc-32233) antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Primary antibodies against collagen type I (1:1000, ab34710), HDAC2 (1:1000, ab12169), HDAC8 (1:1000, ab137474), and HDAC10 (1:1000, ab53096) were purchased from Abcam (Cambridge, MA, USA). Anti-fibronectin antibody (1:1000, MA5-11981) was purchased from Thermo Fisher Scientific (Waltham, MA, USA). Anti-HDAC1 antibody (1:1000, 06–720) was purchased from Merck Millipore (Darmstadt, Germany). Anti-HDAC6 (1:1000, 7612), anti-Smad3 (1:1000, 9523), anti-Smad2 (1:1000, 3103), anti-Smad4 (1:1000, 9515), anti-p-Smad3 (1:1000, 9520), anti-p-JNK (1:1000, 9251), anti-p-p38 (1:1000, 4511), anti-p-ERK1/2 (1:1000, 4370), and anti-p38 (1:1000, 8690) antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Choi S.Y., Piao Z.H., Jin L., Kim J.H., Kim G.R., Ryu Y., Lin M.Q., Kim H.S., Kee H.J, & Jeong M.H. (2016). Piceatannol Attenuates Renal Fibrosis Induced by Unilateral Ureteral Obstruction via Downregulation of Histone Deacetylase 4/5 or p38-MAPK Signaling. PLoS ONE, 11(11), e0167340.

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Colon Cancer Cell Line Maintenance

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Human colon cell lines (HT-29, SW480, SW620, HCT116, and HRT18) were purchased from the American Type Culture Collection (ATCC) in 2010, maintained in our laboratory, and cultured with RPMI-1640 medium supplemented with 10% fetal bovine serum (Life Technologies) at 37 °C in 5% CO₂. The cell lines were routinely tested for presence of mycoplasma with 4,6-diamidino-2-phenylindole staining, and were mycoplasma free.
Antibodies and reagents. Antibodies against RACK1 (sc-17754), Cyclin D1 (sc-450), Bax (sc-20067), cleaved PARP (sc-56196), Bcl-2 (sc-509) and JNK-2 (sc-7345) were purchased from Santa Cruz. Antibodies against SQSTM1 (ab207305), BECN1 (ab62557) and LC3-II (ab192890) were purchased from Abcam. Antibodies against LC3-I/II antibody (#4108) and phospho-JNK-1/2(#9255) were purchased from CST. Horseradish peroxidase-conjugated goat anti-rabbit (#A24531) and anti-mouse IgG antibodies (#A24512) were purchased from Life Technologies. Bafilomycin A1 (B1793) and 3-methyladenine (M9281) were purchased from Sigma-Aldrich.

Xiao T., Zhu W., Huang W., Lu S.S., Li X.H., Xiao Z.Q, & Yi H. (2018). RACK1 promotes tumorigenicity of colon cancer by inducing cell autophagy. Cell Death & Disease, 9(12), 1148.

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Setdb1 Knockdown Immunoblotting Protocol

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The cells were transfected with Setdb1 siRNA for 48 h. Approximately 30 μg protein was separated by 8–12% SDS-PAGE and transferred to PVDF membranes (Millipore). The membranes were probed using the following primary antibodies: NOX4 (1:500; NB110-58849; Novus), beta-Actin (1:2000; CW0096; CWBIO), SETDB1 (1:1000; 11231-1-AP; Proteintech), E2F1 (1:500; sc-193; Santa Cruz Biotechnology), FOXO4 (1:500; sc-5221; Santa Cruz Biotechnology), Lamin B (1:500; sc-6217; Santa Cruz Biotechnology), JNK (1:500; sc-7345; Santa Cruz Biotechnology), p-JNK (1:400; WL01813; WanleiBio), γH2AX (1:000; 2577; Cell signaling technology), p38 (1:500; sc-7972; Santa Cruz Biotechnology), and p-P38 (1:500; sc-17852-R; Santa Cruz Biotechnology). All were used as the manufacturer’s recommendation. The secondary antibodies were horse radish peroxidase-linked anti-mouse, anti-rabbit, or anti-goat IgG for 2 h at room temperature. The membranes were visualized on a Bio-Rad Chemidoc XRS using a Western Bright ECL Kit (Bio-Rad, Berkeley, CA, United States).

Li X., Chen X., Liu Y., Zhang P., Zheng Y, & Zeng W. (2020). The Histone Methyltransferase SETDB1 Modulates Survival of Spermatogonial Stem/Progenitor Cells Through NADPH Oxidase. Frontiers in Genetics, 11, 997.

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Oxidative Stress Biomarker Assays

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MDA assay kit (LPO-586) was purchased from EMD Millipore (Billerica, MA, USA). GSH (CS0260), CAT (CAT100), SOD (19160) and GPx (CGP1) activity assay kits were obtained from Sigma-Aldrich (Merck Millipore, Darmstadt, Germany). Mouse monoclonal antibodies against JNK1/2 (sc-7345) and phospho-JNK1/2 (sc-6254) were purchased from Santa Cruz Biotechnology. All other chemicals were of reagent grade and purchased from Sigma-Aldrich (Merck Millipore), unless stated otherwise.

Lee K.C., Teng C.C., Shen C.H., Huang W.S., Lu C.C., Kuo H.C, & Tung S.Y. (2018). Protective effect of black garlic extracts on tert-Butyl hydroperoxide-induced injury in hepatocytes via a c-Jun N-terminal kinase-dependent mechanism. Experimental and Therapeutic Medicine, 15(3), 2468-2474.

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