B6.129p2 sjl myd88tm1defr j myd88fl fl

IL-12Rβ2^fl/fl mice were generated at the Brown Transgenic Facility, as previously described (21 (link)). B6.129P2(SJL)-MyD88tm1Defr/J (MyD88^fl/fl, Cat #: 008888), B6.129X1-Gt(ROSA)26Sor^tm1(EYFP)Cos/J (eYFP, Cat #: 006148), IL-18^−/− (Cat #: 004130), and C57BL/6 (B6, Cat #: 000664) mice were purchased from Jackson Laboratory. B6.Cg-Tg(CD4-cre)1Cwi N9 (CD4cre, Cat #: 4196), Balb/c (Cat #: BALB-F), and B6.SJL (Cat #: 002014) mice were purchased from Taconic. IL-12Rβ2^fl/fl CD4cre^+/− and MyD88^fl/fl eYFP^+/− CD4cre^+/− mice were generated and maintained in-house, along with littermate controls (IL-12Rβ2^fl/+ CD4cre^+/− and MyD88^fl/+ eYFP^+/− CD4cre^+/−, respectively). Both age- and sex-matched female and male mice (6–26 weeks) were used for these studies; littermates were used as controls for MyD88 cKO and IL-12βR2 cKO mice. All experiments were performed in accordance to the Guide for the Care of Use of Laboratory Animals, as defined by the NIH (PHS Assurance #A3284–01). The Institutional Animal Care and Use Committee (IACUC) of Brown University reviewed, and approved, the animal protocols performed in this study. Animals were housed in an AAALAC-accredited and centralized research facility.

Selp^−/−, CD169-DTR, Csf2^−/− mice, Tg[Hu-miniLCRα1^Gγ^Aγδβ^S] Hba^−/− Hbb^−/− (Berkeley sickle cell mice) and Tg[Hu-miniLCRα1^Gγ^Aγδβ^S] Hba^−/− Hbb^+/− (hemizygous control mice) have been described^{11 (link),14 (link),17 (link),19 (link)}. B6.129P2-Lyz2^tm1(cre)Ifo/J (LysM-Cre), B6.129P2(SJL)-Myd88^tm1Defr/J (Myd88^fl/fl) and B6;129S-Tnf^tm1Gkl/J (Tnf^−/−) mice were purchased from The Jackson Laboratory. Tlr2^−/− and Tlr4^−/− mice were kindly provided by Dr. Eric G. Pamer (Memorial Sloan-Kettering Cancer Center, NY). C57BL/6 CD45.1 and CD45.2 mice were purchased from the National Cancer Institute. Six to eight week old mice were used for experiments. All mice were housed in specific pathogen-free conditions and fed with autoclaved food, and experimental procedures preformed on mice were approved by the Animal Care and Use Committee of Albert Einstein College of Medicine. Germ-free C57/BL6 mice were maintained in sterile isolators with autoclaved food and water in the Gnotobiotic Core of Icahn School of Medicine at Mount Sinai. For fecal transplantation experiments, 100 mg of feces pellets was resuspended in 1 ml of PBS, homogenized, and filtered through a 70 μm strainer. Recipient GF mice were gavaged with 200 μl of the filtrate.