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Human or murine il 1β elisa kits

Manufactured by R&D Systems

The human or murine IL-1β ELISA kits are designed to quantitatively measure the concentration of IL-1β in human or mouse biological samples. The kits utilize the sandwich ELISA technique and include a microplate pre-coated with an antibody specific to IL-1β, as well as all necessary reagents for performing the assay.

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3 protocols using human or murine il 1β elisa kits

1

Inflammatory Cytokine Quantification

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All chemicals used in the present study were purchased from Sigma (St. Louis, MO), unless otherwise noted. Ultrapure LPS, ATP, Caspase-1 inhibitor Ac-YVAD-cmk were purchased from Invivogen (San Diego, CA). Murine or human IL-1β, IL-1α and IL-18 from PeproTech (Rocky Hill, NJ); mouse IL1Ra from Novus Biotech, and human IL-1Ra from PeproTech. Human or murine IL-1β ELISA kits were from R&D system (Minneapolis, MN) or eBioscience (San Diego, California); Antibodies to human IL-1β, human caspase 1, mouse IL-1β and mouse caspase 1 were from R&D Systems or Santa Cruz Biotechnology (Santa Cruz, CA).
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2

Inflammatory Pathway Activation Assays

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All chemicals used in the present study were purchased from Sigma (St. Louis, MO), unless otherwise noted. Ultrapure LPS, Pm3C, ATP, MSU crystal, Caspase inhibitors Z-VAD-FMK, Ac-YVAD-cmk and puno-NLRP3 were purchased from Invivogen (San Diego, CA). Murine or human IL-1β, IL-10 from PeproTech (Rocky Hill, NJ); Anti-CD3 antibody from BD Biosciences (San Diego, CA); Human or murine IL-1β ELISA kits were from R&D system (Minneapolis, MN) or eBioscience (San Diego, California); Alum (Imject-alum) was purchased from Pierce Biochemicals. Antibodies to human IL-1β, human caspase 1, mouse IL-1β and mouse caspase 1 were from R&D Systems or Santa Cruz Biotechnology (Santa Cruz, CA).
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3

Inflammatory Pathway Activation Assays

Check if the same lab product or an alternative is used in the 5 most similar protocols
All chemicals used in the present study were purchased from Sigma (St. Louis, MO), unless otherwise noted. Ultrapure LPS, Pm3C, ATP, MSU crystal, Caspase inhibitors Z-VAD-FMK, Ac-YVAD-cmk and puno-NLRP3 were purchased from Invivogen (San Diego, CA). Murine or human IL-1β, IL-10 from PeproTech (Rocky Hill, NJ); Anti-CD3 antibody from BD Biosciences (San Diego, CA); Human or murine IL-1β ELISA kits were from R&D system (Minneapolis, MN) or eBioscience (San Diego, California); Alum (Imject-alum) was purchased from Pierce Biochemicals. Antibodies to human IL-1β, human caspase 1, mouse IL-1β and mouse caspase 1 were from R&D Systems or Santa Cruz Biotechnology (Santa Cruz, CA).
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