Log In Sign up
The largest database of trusted experimental protocols

Fv3000 confocal microscope

Manufactured by Zeiss
Visit Supplier

The ZEISS FV3000 is a confocal microscope designed for high-resolution imaging. It features a state-of-the-art optical system and advanced detectors to capture detailed fluorescence images. The core function of the FV3000 is to provide researchers with a powerful tool for visualizing and analyzing cellular and subcellular structures with exceptional clarity and resolution.

Automatically generated - may contain errors

Lab products found in correlation

Lipidtox green, by Thermo Fisher Scientific (1 mentions) Slowfade diamond antifade mounting media, by Thermo Fisher Scientific (1 mentions) Nile red, by Santa Cruz Biotechnology (1 mentions) Hcs lipidtox green, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Confocal microscope (860 citations) FV3000 (6 citations) FV3000 microscope (2 citations) FV3000 laser scanning confocal microscope (2 citations)

5 protocols using fv3000 confocal microscope

1

Synaptic Development in Prefrontal Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols
Staining, image acquisition, and analysis were performed as in Ippolito and Eroglu (2010) (link) (Ippolito and Eroglu, 2010 (link)) with adjustments. Synaptic staining was performed in two male/female littermate pairs at P6, P8, P10, P13, and P15 in WT C57BL6/J offspring to determine the normal developmental pattern. Synaptic staining was performed at P8, P15, and P100 in male and female offspring for CON and DEP+MS conditions. Image acquisition was performed in layer 1 (L1) of the ACC from P8, P15, and adult CON and DEP+MS animals. We chose to conduct our analyses in L1 because this layer contains sparse neuronal cell bodies and receives dense axonal inputs from both thalamic and neighboring regions. 5.1 mm-thick confocal images (optical section depth 0.33 µm, 15 sections/scan) were acquired at 60× magnification plus 1.4× optical zoom using an Olympus FV3000 confocal microscope or Zeiss 880.
Block C.L., Eroglu O., Mague S.D., Smith C.J., Ceasrine A.M., Sriworarat C., Blount C., Beben K.A., Malacon K.E., Ndubuizu N., Talbot A., Gallagher N.M., Jo Y.C., Nyangacha T., Carlson D.E., Dzirasa K., Eroglu C, & Bilbo S.D. (2022). Prenatal environmental stressors impair postnatal microglia function and adult behavior in males. Cell reports, 40(5), 111161.
+ Open protocol
+ Expand
2

Synaptic Development in Prefrontal Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols
Staining, image acquisition, and analysis were performed as in Ippolito and Eroglu (2010) (link) (Ippolito and Eroglu, 2010 (link)) with adjustments. Synaptic staining was performed in two male/female littermate pairs at P6, P8, P10, P13, and P15 in WT C57BL6/J offspring to determine the normal developmental pattern. Synaptic staining was performed at P8, P15, and P100 in male and female offspring for CON and DEP+MS conditions. Image acquisition was performed in layer 1 (L1) of the ACC from P8, P15, and adult CON and DEP+MS animals. We chose to conduct our analyses in L1 because this layer contains sparse neuronal cell bodies and receives dense axonal inputs from both thalamic and neighboring regions. 5.1 mm-thick confocal images (optical section depth 0.33 µm, 15 sections/scan) were acquired at 60× magnification plus 1.4× optical zoom using an Olympus FV3000 confocal microscope or Zeiss 880.
Block C.L., Eroglu O., Mague S.D., Smith C.J., Ceasrine A.M., Sriworarat C., Blount C., Beben K.A., Malacon K.E., Ndubuizu N., Talbot A., Gallagher N.M., Jo Y.C., Nyangacha T., Carlson D.E., Dzirasa K., Eroglu C, & Bilbo S.D. (2022). Prenatal environmental stressors impair postnatal microglia function and adult behavior in males. Cell reports, 40(5), 111161.
+ Open protocol
+ Expand
3

Synaptic Development in Prefrontal Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols
Staining, image acquisition, and analysis were performed as in Ippolito and Eroglu (2010) (link) (Ippolito and Eroglu, 2010 (link)) with adjustments. Synaptic staining was performed in two male/female littermate pairs at P6, P8, P10, P13, and P15 in WT C57BL6/J offspring to determine the normal developmental pattern. Synaptic staining was performed at P8, P15, and P100 in male and female offspring for CON and DEP+MS conditions. Image acquisition was performed in layer 1 (L1) of the ACC from P8, P15, and adult CON and DEP+MS animals. We chose to conduct our analyses in L1 because this layer contains sparse neuronal cell bodies and receives dense axonal inputs from both thalamic and neighboring regions. 5.1 mm-thick confocal images (optical section depth 0.33 µm, 15 sections/scan) were acquired at 60× magnification plus 1.4× optical zoom using an Olympus FV3000 confocal microscope or Zeiss 880.
Block C.L., Eroglu O., Mague S.D., Smith C.J., Ceasrine A.M., Sriworarat C., Blount C., Beben K.A., Malacon K.E., Ndubuizu N., Talbot A., Gallagher N.M., Jo Y.C., Nyangacha T., Carlson D.E., Dzirasa K., Eroglu C, & Bilbo S.D. (2022). Prenatal environmental stressors impair postnatal microglia function and adult behavior in males. Cell reports, 40(5), 111161.
+ Open protocol
+ Expand
4

Synaptic Development in Prefrontal Cortex

Check if the same lab product or an alternative is used in the 5 most similar protocols
Staining, image acquisition, and analysis were performed as in Ippolito and Eroglu (2010) (link) (Ippolito and Eroglu, 2010 (link)) with adjustments. Synaptic staining was performed in two male/female littermate pairs at P6, P8, P10, P13, and P15 in WT C57BL6/J offspring to determine the normal developmental pattern. Synaptic staining was performed at P8, P15, and P100 in male and female offspring for CON and DEP+MS conditions. Image acquisition was performed in layer 1 (L1) of the ACC from P8, P15, and adult CON and DEP+MS animals. We chose to conduct our analyses in L1 because this layer contains sparse neuronal cell bodies and receives dense axonal inputs from both thalamic and neighboring regions. 5.1 mm-thick confocal images (optical section depth 0.33 µm, 15 sections/scan) were acquired at 60× magnification plus 1.4× optical zoom using an Olympus FV3000 confocal microscope or Zeiss 880.
Block C.L., Eroglu O., Mague S.D., Smith C.J., Ceasrine A.M., Sriworarat C., Blount C., Beben K.A., Malacon K.E., Ndubuizu N., Talbot A., Gallagher N.M., Jo Y.C., Nyangacha T., Carlson D.E., Dzirasa K., Eroglu C, & Bilbo S.D. (2022). Prenatal environmental stressors impair postnatal microglia function and adult behavior in males. Cell reports, 40(5), 111161.
+ Open protocol
+ Expand
5

Quantifying Lipid Droplet Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were grown on glass coverslips, treated as required and fixed with 4% paraformaldehyde. Lipid droplets were visualized by staining with 0.1 μg/mL Nile Red (Santa Cruz) or HCS LipidTox green (1:200, Invitrogen H34475). For co-staining, cells were fixed with 4% paraformaldehyde, blocked for 1h in IF solution (PBS, 2% BSA, 0.1% Triton-X100), and incubated overnight with mouse a-myc (1:250, Cell Signaling 2276S), followed by donkey anti-mouse Alexa Fluor 594 secondary (1:400, Invitrogen R37115), donkey anti-rabbit Alexa Fluor 488 (1:400, Invitrogen A21206) and LipidTox green (1:200, Invitrogen H34475) for 1 hour at room temperature. Finally, cells were incubated with Hoechst 33342 nuclear stain for 5 minutes, and mounted with SlowFade Diamond antifade mounting media (Life Technology S36968). Slides were imaged on an Olympus FV3000 confocal microscope or a Zeiss Axioskope wide field microscope. Lipid droplet numbers and sizes were quantified with ImageJ and the particle counting plugin.
VandeKopple M.J., Wu J., Auer E.N., Giaccia A.J., Denko N.C, & Papandreou I. (2019). HILPDA regulates lipid metabolism, lipid droplet abundance, and response to microenvironmental stress in solid tumors.. Molecular cancer research : MCR, 17(10), 2089-2101.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!