Trio 3.0t mri

During each fMRI scan an Echo-Planar imaging (EPI) sequence was used for data collection, and T2*-weighted images were recorded per run using a Siemens TRIO 3.0T MRI scanner [repetition time (TR) = 2000 ms; echo time (TE) = 30 ms; flip angle = 90°; FoV = 220 × 220 mm²; matrix size = 64 × 64; 32 interleaved 3 mm-thick slices; in-plane resolution = 3.4 × 3.4 mm²; inter slice skip = 0.99 mm]. Additionally, anatomical images (256 × 256 × 176) with 1 × 1 × 1 mm³ resolution were obtained by a T1-weighted three-dimensional magnetization prepared rapid gradient echo (MPRAGE) sequence (inversion time = 900 ms; TR = 1900 ms; TE = 2.52 ms; flip angle = 9°).

We used a Siemens TRIO 3.0T MRI scanner (Siemens Magnetom Trio TIM, Erlangen, Germany) to collect functional imaging data. Head movement was restricted using foam cushions. T1-weighted images were recorded with a total of 176 slices at a thickness of 1 mm and in-plane resolution of 0.98 × 0.98 mm(TR = 1900 ms; TE = 2.52 ms; flip angle = 9°; FOV = 250 × 250 mm²). An Echo-Planar imaging (EPI) sequence was used for data collection (TR = 2000 ms; TE = 30 ms; flip angle = 90°; FoV = 192 × 192 mm2 (link); matrix size = 64 × 64; voxel size = 3 × 3 × 3 mm³; interslice skip = 0.99 mm; Slices = 32).

The MRI scanner used was a standard 3.0 T Signa system (TRIO 3.0 T MRI; Siemens MAGNETOM, Erlangen, Germany). Anatomical images were acquired in multiple planes prior to and after sonication using a T2-weighted fast spin echo sequence (TE = 16 ms; TR = 1,000 ms; ETL = 4; BW = 16 kHz; matrix, 1846256; slice = 1 mm; NEX = 2; FOV = 5 cm) to evaluate whether signs of tissue damage were present after exposure. The rats were anesthetized with 30% chloral hydrate during the imaging procedure. A 7.5-cm diameter surface coil was placed under the head. Sonication was performed through a hole in the coil that was filled with a bag containing degassed water. A gradient echo sequence was used to aim the beam at the brain. Following each sonication, T2-weighted fast spin echo images were obtained and repeated after an intravenous bolus injection of meglumine gadopentetate MR contrast agent (0.1 ml/kg; Consun, Guangzhou, China) to detect and evaluate the opening of the BBB.