Chromium single cell 5 d j solution

Manufactured by 10x Genomics

The Chromium Single Cell V(D)J Solution is a lab equipment product offered by 10x Genomics. It is designed to enable the analysis of the adaptive immune system by capturing and sequencing the variable regions of T-cell receptor (TCR) and B-cell receptor (BCR) genes from individual cells.

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Lab products found in correlation

Gentlemacs dissociator and tumor infiltrating lymphocyte kit, by Miltenyi Biotec (1 mentions) Novaseq 6000 platform, by Illumina (1 mentions) Novaseq 6000 system, by Illumina (1 mentions) Collagenase p, by Merck Group (1 mentions) Dnase 1, by Roche (1 mentions)

2 protocols using chromium single cell 5 d j solution

Single-cell Profiling of Tumor Samples

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For single-cell preparation, tumor tissue was dissociated with the gentleMACS Dissociator and Tumor infiltrating Lymphocyte Kit (Miltenyi Biotec) according to the manufacturer's protocol. The cells were then cryopreserved in liquid nitrogen until use. All samples showed a viability of around 90% on average after thawing. We performed 5′ gene expression profiling on the same single-cell suspension using the Chromium Single Cell V(D)J Solution from 10x Genomics according to the manufacturer's instructions. Up to 8,000 cells were loaded onto a 10x Genomics cartridge for each sample. Cell-barcoded 5′ gene expression Libraries were constructed and sequenced at a depth of approximately 50,000 reads per cell using the NovaSeq 6000 platform (Illumina).

An M., Mehta A., Min B.H., Heo Y.J., Wright S.J., Parikh M., Bi L., Lee H., Kim T.J., Lee S.Y., Moon J., Park R.J., Strickland M.R., Park W.Y., Kang W.K., Kim K.M., Kim S.T., Klempner S.J, & Lee J. (2024). Early Immune Remodeling Steers Clinical Response to First-Line Chemoimmunotherapy in Advanced Gastric Cancer. Cancer Discovery, 14(5), 766-785.

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Profiling Tumor Transcriptomes in PDXs

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Single‐cell RNA and TCR sequencing were conducted to profile transcriptional changes in pre‐ (n = 12) and posttreatment (n = 3 PD‐1i, n = 4 PI3K/mTORi, n = 4 PI3K/mTORi+PD‐1i) tumour samples collected from CD34+ HSC humanized PDXs. Tumour biopsies were obtained using 2 mm diameter punch needles (Kai Medical, A01020302) and processed into single‐cell suspensions. The samples underwent mechanical and enzymatic dissociation (2 mg/mL Collagenase P, Sigma Aldrich and .2 mg/mL DNAse I, Roche). Single‐cell TCR sequencing and 5′ gene expression profiling were performed using the Chromium Single Cell V(D)J Solution from 10x Genomics (8000 viable cells/sample). Cell‐barcoded libraries were sequenced on an Illumina NovaSeq6000 system and reads were aligned to the GRCh38 human reference genome using the Cell Ranger Single‐cell software suite.

De Wispelaere W., Annibali D., Tuyaerts S., Messiaen J., Antoranz A., Shankar G., Dubroja N., Herreros‐Pomares A., Baiden‐Amissah R.E., Orban M., Delfini M., Berardi E., Van Brussel T., Schepers R., Philips G., Boeckx B., Baietti M.F., Congedo L., HoWangYin K.Y., Bayon E., Van Rompuy A., Leucci E., Tabruyn S.P., Bosisio F., Mazzone M., Lambrechts D, & Amant F. (2024). PI3K/mTOR inhibition induces tumour microenvironment remodelling and sensitises pS6high uterine leiomyosarcoma to PD‐1 blockade. Clinical and Translational Medicine, 14(5), e1655.

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