I control 3

Cells were seeded with eight technical replicates on PLO-coated black 96-well plates with 15,000 cells per well. CellTiter-Blue^® Cell Viability (CTB) Assay (Promega, WI, USA, G8081) was used to determine the metabolic activity of the cells. Two biological replicates were employed for this study. According to the manufacturer’s instructions, at the designated sample collection time points (Fig. 2), CellTiter-Blue^® reagent was added to the cells and incubated for 2 h at 37 °C. The assay is based on the conversion of the redox dye resazurin into resorufin, a fluorescent end-product. The resulting fluorescence intensity (excitation: 560 nm, emission: 590 nm) was recorded with a Tecan infinite M200 plate reader and processed with the i-control 3.4.2.0 software (Tecan, Männedorf, Switzerland). The CytoTox 96^® Non-Radioactive Cytotoxicity (LDH) Assay (Promega, WI, USA, G1782) was used to determine cell cytotoxicity. According to the manufacturer’s instructions, at the designated sample collection time points, CytoTox 96^® working solution was added to wells and incubated for 30 min at room temperature. The assay is based on the quantification of extracellular LDH through the directly proportional formation of a dye. Absorbance was recorded at 490 nm with a Tecan infinite M200 plate reader and processed with the i-control 3.4.2.0 software (Tecan, Männedorf, Switzerland).