Dhea s

Authentic standards were acquired for Ch-S (Avanti Polar Lipids #700016), DHEA-S (Cayman chemical 15873), pregnenolone-sulfate (Sigma P162), and estradiol-sulfate (Sigma E9505). Additional standards were synthesized and authenticated as noted below.

Dimethyl sulfoxide (DMSO) was purchased from Alfa Aesar (Haverhill, MA). PFOA (CAS: 335–95-5) was purchased from Thermo Fisher Scientific Inc. (Waltham, MA). PFOS (CAS: 2795–39-3) was supplied by Sigma-Aldrich (St Louis, MO). GenX (CAS: 62037–80-3) was purchased from Manchester Organics (Runcorn, UK). PFNA (CAS: 375–95-1) and PFHxS (CAS: 3871–99-6) were both acquired through Frontier Scientific Inc. (Logan, UT). PFNS (CAS: 98789–57-2) was purchased from Toronto Research Chemicals (Toronto, ON). All PFAS stocks were prepared fresh in DMSO prior to each experiment (5–100 mM). Glucose-6-phosphate, Glucose-6-phosphate dehydrogenase, β-nicotinamide adenine dinucleotide phosphate (NADP⁺), and dehydroepiandrosterone-d₅-3-sulfate (DHEA-S-d5) were all purchased from Sigma Aldrich (St. Louis, MO). The fluorescent probe substrate, dibenzylfluorescein (DBF, CAS: 97744–44-0) and DHEA-S (CAS: 1099–87-2) were supplied by Cayman Chemical Company (Ann Arbor, MI). The metabolite standard, 16α-hydroxy DHEA-S, was purchased from Steraloids (Newport, RI). The CYP3A7 Bactosomes (catalog number: CYP/EZ060) were purchased from Cypex Ltd. (Scotland, UK) where the recombinant CYP3A7 enzyme was co-expressed with the human cytochrome P450 reductase in E. coli and then supplemented with the human cytochrome b₅.