Approximately 2 X105 infected erythrocytes were collected and lysed in 0.5% saponin. The pellet was resuspended in SDS buffer containing 2% β-mercaptoethanol, and separated by SDS-PAGE. After transfer to a nitrocellulose membrane, blots were blocked in 5% milk, and probed with either mouse anti-GFP (1:10,000) or mouse anti- hemagglutinin (HA) epitope (Santa Cruz Biotechnology; 1:10,000 dilution), and subsequently with rabbit or goat anti-mouse IgG conjugated with HRP (secondary 1:1000), and developed on film with SuperSignal West Femto substrate (ThermoFisher Scientific). Plasmodium aldolase was detected using a rabbit anti-aldolase (Abcam) directly conjugated to HRP (1:40,000 dilution).
Rabbit anti aldolase
Manufactured by Abcam
Rabbit anti-aldolase is a primary antibody that can be used to detect the presence of the aldolase enzyme in various biological samples. Aldolase is an enzyme that plays a crucial role in the glycolysis pathway, catalyzing the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate. This antibody can be a valuable tool for researchers studying the expression and distribution of aldolase in different cell types or tissues.
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Lab products found in correlation
Supersignal west femto substrate, by Thermo Fisher Scientific (1 mentions)
Mouse anti gfp, by Santa Cruz Biotechnology (1 mentions)
Ecl western blotting kit, by GE Healthcare (1 mentions)
Anti ha, by Roche (1 mentions)
Trans blot sd semi dry electrophoretic transfer cell, by Bio-Rad (1 mentions)
Mouse anti gfp, by Merck Group (1 mentions)
Sds loading buffer, by Bio-Rad (1 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
2 protocols using rabbit anti aldolase
1
Immunoblot Analysis of Plasmodium Proteins
2
Western Blot Analysis of Malaria Parasites
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Synchronized schizont-stage parasites were isolated by treatment of infected RBC (iRBCs) with 0.15% saponin in PBS on ice for 10 min. Parasite pellets were washed by 1× PBS twice, then dissolved in 100 μl 1× SDS-loading buffer (Bio-Rad) and boiled at 100°C for 5 min. Total proteins were loaded onto SDS-PAGE gels with suitable amount according to the theoretical molecular weight of target proteins. Proteins were transferred to a PVDF membrane by semi-dry (Trans-Blot® SD Semi-Dry Electrophoretic Transfer Cell) for 2 h at room temperature or wet transfer (Trans-Blot® Cell) overnight at 4°C, and visualized by exposing with the Bio-Rad ChemiDoc XRS system. The antibody used in this study was mouse anti-GFP (Sigma), anti-HA (Roche) and rabbit anti-aldolase (Abcam). The experiment was done with ECL western blotting kit (GE healthcare).
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