Ventricle tissue sections were incubated with primary antibodies against cleaved caspase3 (Servicebio, GB11532) and GRP78 (Servicebio, GB11098), and the nuclei were stained with DAPI. The results were analyzed by Image-Pro Plus 6.0 software. More than three ventricle sections from different animals were randomly selected to quantify the expression of cleaved caspase3 and GRP78. The mean optical density of the protein was calculated as follows: sum of the optical density values/total area.
Gb11098
Manufactured by Wuhan Servicebio Technology
Sourced in China
GB11098 is a centrifuge designed for laboratory applications. It is capable of separating sample components based on their density and sedimentation rate.
Automatically generated - may contain errors
Lab products found in correlation
Gb11532, by Wuhan Servicebio Technology (1 mentions)
Gb11297, by Wuhan Servicebio Technology (1 mentions)
Gb23303, by Wuhan Servicebio Technology (1 mentions)
A21428, by Thermo Fisher Scientific (1 mentions)
Goat serum, by Beyotime (1 mentions)
Laser confocal microscope, by Olympus (1 mentions)
Gb22303, by Wuhan Servicebio Technology (1 mentions)
Gb21301, by Wuhan Servicebio Technology (1 mentions)
4 protocols using gb11098
1
Quantifying Apoptosis and ER Stress in Ventricle
2
Quantifying ATF6 and GRP78 Expression
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Immunohistochemistry for activating transcription factor 6 (ATF6) (GB11297; Servicebio, Wuhan, China) and glucose-regulated protein78 (GRP78) (GB11098; Servicebio, Wuhan, China) was performed on formalin-fixed paraffin-embedded tissue sections. Peroxidase-conjugated goat anti-rabbit IgG (GB23303; Servicebio, Wuhan, China) was used as the secondary antibody. The protocols of immunohistochemical staining have been described previously [26 (link)]. The cell nuclei were colored purple-blue, and positive products were tan or yellow. Three photographs were selected randomly for each slide and analyzed using ImageJ software (National Institutes of Health, Bethesda, MD).
3
Immunohistochemical Analysis of GRP78 in Mouse Heart
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The mouse heart sections were deparaffinized with xylene and then dewatered with ethanol. After heat-induced antigen retrieval, the cell membrane was permeabilized with 0.1% Triton X-100, and non-specific sites were blocked with goat serum (C0265; Beyotime Biotechnology) for 30 min at a room temperature. The sections were then incubated with rabbit polyclonal antibodies against glucose-regulated protein 78 (GRP78) (1:200, GB11098; Servicebio, Wuhan, China) overnight at 4°C, followed by incubation with fluorescein-conjugated secondary antibodies (1:200, A-21428; Invitrogen) for 1 h at a room temperature in the dark. Afterwards, the nuclei were stained with DAPI for 5 min at a room temperature. The images were captured using a fluorescence microscope (Ti-S; Nikon, Tokyo, Japan).
4
Immunofluorescence Analysis of ERS Markers
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The expressions of ERS marker proteins glucose-regulated protein 78 (GRP78), glucose-regulated protein 75 (GRP75), and C/EBP homologous protein (CHOP) in HK-2 cells were detected by immunofluorescence. Tissue slides were dewaxing, rehydrated, antigen retrieval, and blocking. The rehydrated paraffin sections (4 μm) were washed with PBS, anti-CHOP (1:100, PA5-104528, Thermo Fisher Scientific, USA), GRP75 (1:100, ab227215, abcam, UK), and GRP78 (1:200, GB11098, Servicebio, China) were incubated overnight at 4 °C, and then stained with FITC-labeled goat anti-rabbit IgG (1:100, GB22303, GB21301, GB22303, Servicebio, China) for 30 min. Nuclei were incubated with DAPI for 10 min at room temperature. Finally, the sections were analyzed by a confocal laser microscope (Olympus Corporation, Japan). The antibodies against GRP78, GRP75, and CHOP exhibited a strong green fluorescence indicating positive expression, while DAPI staining appeared as blue fluorescence representing the cell nuclei.
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The expressions of ERS marker proteins glucose-regulated protein 78 (GRP78), glucose-regulated protein 75 (GRP75), and C/EBP homologous protein (CHOP) in HK-2 cells were detected by immunofluorescence. Tissue slides were dewaxing, rehydrated, antigen retrieval, and blocking. The rehydrated paraffin sections (4 μm) were washed with PBS, anti-CHOP (1:100, PA5-104528, Thermo Fisher Scientific, USA), GRP75 (1:100, ab227215, abcam, UK), and GRP78 (1:200, GB11098, Servicebio, China) were incubated overnight at 4 °C, and then stained with FITC-labeled goat anti-rabbit IgG (1:100, GB22303, GB21301, GB22303, Servicebio, China) for 30 min. Nuclei were incubated with DAPI for 10 min at room temperature. Finally, the sections were analyzed by a confocal laser microscope (Olympus Corporation, Japan). The antibodies against GRP78, GRP75, and CHOP exhibited a strong green fluorescence indicating positive expression, while DAPI staining appeared as blue fluorescence representing the cell nuclei.
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