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2 protocols using mouse pd l1 antibody mab90781 100

1

Comprehensive Signaling Pathway Analysis

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The human PD-L1 (13684) Rabbit mAb, c-JUN (60A8) Rabbit mAb (9165S), Phospho-JNK (Thr183/Tyr185) (81E11) Rabbit mAb (4668T), Phospho-c-JUN (Ser63) II Antibody (9261S), NAE1 Rabbit mAb (14321S), p-ERK1/2 Rabbit mAb (4376S), ERK1/2 Mouse mAb (9106S), p-AKT Rabbit mAb (4060S), AKT Rabbit mAb (4691S), β-CATENIN Rabbit mAb (8480S), Cleaved-PARP Rabbit mAb (9541S) Cleaved-Caspase3 Rabbit mAb (9661S) and Phospho-c-FOS (Ser32) (5348T) were purchased from Cell Signaling Technology. UBA3 (ab124728) Rabbit mAb was purchased from Abcam. Mouse PD-L1 antibody (MAB90781-100) was purchased from R&D Systems. APC-conjugated anti-human PD-L1 (329708), APC anti-mice CD3 (100235), FITC anti-h/m Gramzyme B (515403), PE anti-mIFN-γ (505808), FITC-anti-mCD4 (10509), PE anti-mCD8a (100707), Fixation Buffer (420801) and Permeabilization Wash Buffer (10×) (421002) were purchased from Biolegend. Collagenase (C0130-500MG), β-ACTIN Mouse mAb (A5441) was purchased from Sigma. MEK inhibitor (trametinib) and JNK inhibitor (SP600125) were purchased from MedChem Express. AKT inhibitor (MK2206) and MLN4924 (S7109) were purchased from Selleck.
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2

Immunophenotyping and Protein Regulation Assay

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Anti-PD-L1 (E1L3N) rabbit mAb (13,684), anti-CDK5 (2506S) and anti-ubiquitin (3933S) were purchased from Cell Signaling Technology. Mouse PD-L1 antibody (MAB90781-100) was purchased from R&D systems. Anti-TRIM21 (12,108-1-AP) was purchased from Proteintech. Fluorochrome-conjugated monoclonal antibodies including anti-CD3, anti-CD4, anti-CD8, anti-CD45, anti-PD-1 and live/dead were purchased from BD Pharmingen. In vivo mab anti-mouse PD-L1 was purchased from BioXcell. Cycloheximide (CHX) was purchased from Selleck. MG132 was purchased from MedChemExpress. Silver stain kit was purchased from Solarbio LIFE SCIENCES.
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