Dried plants were ground to a powder with a pulverizator (National Mixer Grinder Mx-119N, Japan). 50 g of powder was then macerated 48 h (at room temperature) in 70% ethanol in a 1/10 (w/v) ratio. The material was filtered through a Millipore filter of 0.2 μm (Acrodisc, USA). The filtrate was concentrated under reduced pressure at less than 40°C using a rotary evaporator (Buchi Rotavapor R-200/205, Switzerland) to obtain a crude residue.
Rotavapor r 200 205
Manufactured by Büchi
Sourced in Switzerland
The Rotavapor R-200/205 is a rotary evaporator designed for the efficient removal of solvents from samples. It features a temperature-controlled heating bath, a rotating evaporation flask, and a condenser system to facilitate the evaporation and recovery of volatile components.
Automatically generated - may contain errors
6 protocols using rotavapor r 200 205
1
Ethanolic Extraction of Dried Plant Powder
2
Extraction and Lyophilization of CA Compound
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
CA powder (10 g) was dissolved in 50 mL methanol and then incubated for 3 days at 30°C. After that, the resultant product was filtered using Whatman filter paper No. 1. The resulting extract was concentrated and lyophilized using a rotary evaporator (BUCHI® Rotavapor R-200/205, Flawil, Switzerland) under decreased pressure at 40°C and a freeze-dry vacuum system (Labconco, Kansas, MO, USA) [11 (link),12 (link)].
3
Extraction and Yield Evaluation of Ceylon Cinnamon
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Locally produced cinnamon bark C. verum (Ceylon cinnamon) was purchased from a spice store in Egypt, identified and voucher specimen number was placed by the members of the Department of Pharmacology and Chemotherapeutics, Faculty of Veterinary Medicine, Damanhour University, Egypt. The voucher specimen number of C. verum is A 0177107 (DPV). The bark was dried in an electric drying oven at 30 °C (Sanyo, Osaka, Japan) and pounded using a 60–80 mm mesh. The finely ground C. verum powder (100 g) was dissolved in 250 mL of each solvent (acetone and ethyl acetate) and then stirred at 25 °C for 72 h as described elsewhere [9 ,35 ]. The obtained solutions were filtered using Whatmann filter paper no. 1., concentrated using a rotary evaporator (RotavaporR-200/205, BUCHI®, Flawil, Switzerland) and lyophilized using a Freeze dry vacuum system (Labconco, Kansas City, MO, USA) [7 (link),8 ]. Crude extracts were weighed followed by the addition of 1 mL of DMSO to 100 mg of the extract to be stored at −30 °C. The resulting extract weight was 8.34 g and the yield percentage was calculated using the following equation [38 (link)]:
4
Extraction of Piper Plant Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Leaves of P. betle L. and P. sarmentosum Roxb. and dried seeds of P. nigrum L. were collected from central Thailand from April to May 2020 (Figure 5 ). All plants were identified and stored at the Sireeruckhachati Nature Learning Park, Faculty of Pharmacy, Mahidol University. The plant serial numbers were as follows: P. betle L., PBM–005510–1; P. nigrum L., PBM–005504–6; and P. sarmentosum Roxb., PBM–005491–2. The leaves of PB (5000 g) and PS (6000 g) were washed with tap water and oven-dried at 60–70 °C for 48–72 h. The seeds of P. nigrum (1000 g) were dried and ground to small pieces. The test plants were extracted by treating with 97% ethanol (Sigma-Aldrich, St. Louis, MO, USA) at room temperature (RT) for a period of 3 days, filtered through sterile gauze, and evaporated to dryness under reduced pressure at 40 °C using a rotary evaporator (Rotavapor R-200/205, BÜCHI, Flawil, Switzerland). The extracts were then lyophilized using a Freeze Dry Vacuum System (Labconco, Kansas City, MO, USA). The final crude extracts were weighed, and the yields of the extracts were calculated based on their dry weights. The crude extracts were dissolved in dimethyl sulfoxide (DMSO) at 100 mg/mL before use.
5
Preparation of Lipid-Based Drug Delivery Systems
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The three types of SLNs (containing AmB, PAR, or SSZ) were prepared as previously described (5, 17) . Briefly, 50 mg of drug (AmB, PAR, or SSZ) and 120 mg of lecithin were initially dissolved in a 40-mL mixture of chloroform and methanol at a 1:1 ratio, along with 200 mg each of cocoa butter and beeswax. The solvent was evaporated off using Rotavapor® R-200/205 (Büchi, Switzerland) at 50°C. The resulting druglipid matrix was melted in 20 mL of ethyl acetate at 70°C and added to 40 mL of 2.5% w/v sodium cholate solution at the same temperature. The mixture was homogenized at 10,000 rpm using an IKA T 25® homogenizer (IKA, Germany) for 6 min. A total of 60 mL of water at 70°C was then added slowly to the mixture with continuous stirring for 20 min after which the organic solvent was evaporated off using the Rotavapor at 70°C.
6
Date Palm Seed Cultivar Extraction
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Date palm fruits were obtained from the M'zab oasis of the Ghardaia region in southern Algeria. The seeds of eight different cultivars locally known as Ourous (OUR), Tazizaout (TAZ), Tazarzeit (TAR), Tazoughart (TAG), Ouaouchet (OUC), Oukasaba (OUK), Delat (DEL) and Tamezwertn'telet (TWT) were directly isolated from date fruits collected at the mature stage.
Removed seeds were soaked in water, washed to free them from any adhering date flesh and then dried at 50 °C for 24 h. The dried seeds of each cultivar were separately ground into a fine powder (250 µm of diameter) with a coffee grinder after having crushed them to small pieces using a mortar. The extracts were prepared from each sample by continuous agitation. Briefly, the powder was extracted three times at room temperature for 24 h, in acetone/H 2 O (75/25, v/v). The mixture was centrifuged and then filtered and the obtained filtrates were evaporated in a rotary evaporator (Rotavapor R-200/205, Buchi, Flawil, Switzerland) at 40 °C. The aqueous extracts were frozen at -20 °C in a freezer for 24 h and finally lyophilized using a Christ Alpha 1-4 LD freeze-dryer over 10 days. A powdery residue was obtained and stored at -20 °C in an airtight container until use. The yield of extraction was estimated for each date seed cultivar.
Removed seeds were soaked in water, washed to free them from any adhering date flesh and then dried at 50 °C for 24 h. The dried seeds of each cultivar were separately ground into a fine powder (250 µm of diameter) with a coffee grinder after having crushed them to small pieces using a mortar. The extracts were prepared from each sample by continuous agitation. Briefly, the powder was extracted three times at room temperature for 24 h, in acetone/H 2 O (75/25, v/v). The mixture was centrifuged and then filtered and the obtained filtrates were evaporated in a rotary evaporator (Rotavapor R-200/205, Buchi, Flawil, Switzerland) at 40 °C. The aqueous extracts were frozen at -20 °C in a freezer for 24 h and finally lyophilized using a Christ Alpha 1-4 LD freeze-dryer over 10 days. A powdery residue was obtained and stored at -20 °C in an airtight container until use. The yield of extraction was estimated for each date seed cultivar.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!