1 2 dilauroyl sn glycero 3 phosphocholine

Arachidonic acid, 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS), 1,2-dilauroyl-sn-glycero-3-phosphocholine (DLPC), 5-aminolevulinic acid (ALA), NADP⁺, and NADPH were purchased from Sigma–Aldrich (St. Louis, MO, USA). Paclitaxel was purchased from Santa Cruz Biotechnology (Dallas, TX, USA), and the primary vascular eicosanoid LC–MS mixture and (±)11,12-epoxy-5Z,8Z,14Z-eicosatrienoic acid (11,12-EET) were purchased from Cayman Chemicals (Ann Arbor, MI, USA). 6α-HydroxyPaclitaxel was kindly provided by Professor Sang Kyum Kim of Chungnam National University. Ni²⁺-nitrilotriacetate (NTA) agarose was purchased from Qiagen (Hilden, Germany). Other chemicals of the highest commercially available grade were used. Escherichia coli DH5α was purchased from Invitrogen (Carlsbad, CA, USA). Rat NADPH-P450 reductase (NPR) was heterologously expressed in E. coli (TOPP3 strain) and purified as described previously [23 (link)].

Progesterone, pregnenolone, 17-OH Progesterone, 17-OH pregnenolone, androstenedione, dehydroepiandrosterone, protease inhibitor cocktail, cholesterol oxidase, and 1,2-dilauroyl-sn-glycero-3-phosphocholine were purchased from Sigma–Aldrich. Ni²⁺–NTA–agarose was purchased from Qiagen. 5-Aminolevulinic acid was purchased from Frontier Scientific. IPTG and CHAPS were purchased from Anatrace. Other chemicals were of the highest grade commercially available. Peptides were purchased from New England Peptides, with purity and identity analysis provided (Fig. S8):
with the numbering of the residues shown from the primary sequence.