Ultrasensitive mouse elisa kit

C57BL/6J male mice (n = 12) of 15–21 weeks of age (average weight 23 ± 0.5 g) were divided into two groups. The first group was fed with a low fat (LF) chow D12450B as a control and the second was kept on a high fat (HF) diet D12492. The percentage of fat content in the diets were 10 kcal% fat and 60 kcal% fat, respectively. Mice were kept for 5 weeks in total on the diet. Glucose, insulin, lipids and weight were controlled weekly after 6 h of fasting. The study protocol (ID: ALR2015-2016) was approved by the Ethics Committee for Animal Experimentation at Juntendo University, Japan (2015–2016).
Triglycerides and blood glucose levels were measured using CardioChek^® PA (catalogue No. 0197) and the compatible PTS Panel^® test strips. Insulin was measured using the Ultrasensitive Mouse ELISA kit (Mercodia, Uppsala, Sweden, article No. 10-1249-01).

Adult white Balb/c male mice (22±3g), six to seven mice per group, were given a high fat diet daily (HFD) ad libitum, and a week later, injected with a single dose of alloxan (50 mg/Kg; IP/SC), and divided into three equal groups (seven mice per group). Diabetes was confirmed after two consecutive blood glucose readings of > 13mM taken 24 hours apart. The groups were gavaged a daily dose of probucol powder, probucol microcapsules, or probucol-ursodeoxycholic acid microcapsules (80 mg/Kg) for three months, and euthanized at the end of the study (on day 91) using Isoflurane gas as per approved animal ethics protocols, and then blood, tissues, and faeces collected for blood glucose and probucol analyses (Fig 2).
Blood glucose concentrations (mM) were measured via tail vein venepuncture daily and data analysed using Accu-check glucometers (Roche Laboratories, Basel, Switzerland). Insulin was measured using an ultrasensitive mouse Elisa kit (Mercodia, Switzerland), while the homeostasis model assessment for insulin resistance (HOMA-IR) and β-functions (HOMA-β) was measured mathematically as described elsewhere [20 (link), 21 (link)].

Circulating levels of HDL-cholesterol, LDL-cholesterol, triglycerides, free fatty acids, ALP, ALT, and fructosamine were determined with an autoanalyzer (Cobas Roche Diagnostic, Basel, Switzerland). Insulin was assayed with an ultrasensitive mouse ELISA kit (Mercodia, Uppsala, Sweden). Serum glycerol and hepatic triacylglycerol were determined by using enzymatic colorimetric kits (Sigma–Aldrich, Saint-Quentin-Fallavier, France). Organ lipid content was determined according to Dole and Meinertz [39 (link)]. Protein content was determined with the BCA protein assay kit (Pierce, Rockford, IL, USA). DNA content was determined with a fluorometric method.