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Transwell 0.4 μm

Manufactured by Corning
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The Transwell 0.4 μm is a laboratory equipment product manufactured by Corning. It is a permeable support system designed for cell culture applications. The product features a membrane with a pore size of 0.4 micrometers, which allows for the passage of small molecules and solutes between the upper and lower chambers of the device.

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Corning Costar 12 mm 0.4 μm Transwells® Transwells (0.4 μm polycarbonate) 0.4 μm Polycarbonate membrane Transwell® 24-well plates 24-well Transwell insert with a 0.4 μm pore Transwell permeable polycarbonate inserts (0.4 μm) Transwell® chamber with a 0.4-μm pore size permeable membrane Transwell chambers (0.4 μm pore polycarbonate filters; 0.4 μm pore Transwell® filter systems 0.4-μm pore Transwell inserts Transwell permeable support with 0.4 μm pore

2 protocols using transwell 0.4 μm

1

Cerebral Endothelial Cell Culture Protocol

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When they reached confluence, BBCECs were treated with trypsin-EDTA, dissociated and seeded (4 x 105 cells/mL) on rat-tail collagen-coated filters (Costar Transwell 0.4 μm, 12-well format). Filters were placed above wells containing rat GCs. The culture medium was the same as the one used for ECs alone and was changed every 2 days. In these conditions, ECs formed a confluent monolayer after 5 days and were used 7 days after confluence. In total, 12 days were required for getting the specific properties of cerebral endothelium [8 (link)].
Drolez A., Vandenhaute E., Julien S., Gosselet F., Burchell J., Cecchelli R., Delannoy P., Dehouck M.P, & Mysiorek C. (2016). Selection of a Relevant In Vitro Blood-Brain Barrier Model to Investigate Pro-Metastatic Features of Human Breast Cancer Cell Lines. PLoS ONE, 11(3), e0151155.
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2

HUVEC-Pericyte Co-culture Angiogenesis Assay

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When the HUVECs culture reached the confluence, cells were treated with trypsin-EDTA, seeded onto Matrigel™-coated filters (Costar Transwell 0.4 μm, 12-well format) (8 x 104 cells/cm2) and put above wells containing or not pericytes or rat GCs. Medium was changed every 2 days; cells were used for experiment after 6 days of co-culture.
Drolez A., Vandenhaute E., Julien S., Gosselet F., Burchell J., Cecchelli R., Delannoy P., Dehouck M.P, & Mysiorek C. (2016). Selection of a Relevant In Vitro Blood-Brain Barrier Model to Investigate Pro-Metastatic Features of Human Breast Cancer Cell Lines. PLoS ONE, 11(3), e0151155.
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