201 electron microscope

Manufactured by Philips

Sourced in Netherlands

The Philips 201 electron microscope is a versatile instrument designed for high-resolution imaging and analysis of small-scale structures. It utilizes a focused beam of electrons to generate detailed images, providing a powerful tool for researchers and scientists across various fields. The core function of the 201 electron microscope is to enable the observation and examination of specimens at the nanoscale level, allowing for the study of materials, surfaces, and biological samples with exceptional detail and precision.

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Lab products found in correlation

Em uc7 ultramicrotome, by Leica (2 mentions) Poly bed 812, by Polysciences (2 mentions) Intense m, by Cytiva (1 mentions) Cy5 conjugated anti rabbit igg, by Jackson ImmunoResearch (1 mentions) Tritc conjugated phalloidin, by Merck Group (1 mentions) Lsm 510 confocal microscope, by Zeiss (1 mentions)

4 protocols using 201 electron microscope

Ultrastructural Analysis of CHIKV Infection

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Vero cell monolayers were prepared and infected with EILV/CHIKV or formalin-inactivated EILV/CHIKV for 1hr at 4°C. Following cold-binding to cells at a MOI of 1000 PFU/cell and fixation for 0 or 15 seconds, or 5 minutes after incubation at 37°C, the cells were processed for thin-section EM as previously described (22 (link)). Infected Vero (3 time points including 0, 15 secs, and 5 mins) cell monolayers were fixed in 2.5% formaldehyde/0.1% glutaraldehyde in 0.05 M cacodylate buffer pH 7.3 containing 0.03% trinitrophenol and 0.03% CaCl2 for at least 1 hour at room temperature. Monolayers were then washed in 0.1 M cacodylate buffer, scraped and pelleted by centrifugation. Pellets were post-fixed in 1% OsO4, 0.1 M cacodylate buffer, pH 7.3, washed with distilled water, en bloc stained with 2% aqueous uranyl acetate for 20 minutes at 60°C, then dehydrated in graded series of ethanol and embedded in Poly/Bed 812 (Polysciences, Warrington, PA). Ultrathin sections were cut on Leica EM UC7 ultramicrotome (Leica Microsystems, Buffalo grove, IL), stained with lead citrate and examined on a Philips (Eindhoven, Netherlands) 201 electron microscope at 60 KV.

Erasmus J.H., Auguste A.J., Kaelber J.T., Luo H., Rossi S.L., Fenton K., Leal G., Kim D.Y., Chiu W., Wang T., Frolov I., Nasar F, & Weaver S.C. (2016). A Chikungunya Fever Vaccine Utilizing an Insect-Specific Virus Platform. Nature medicine, 23(2), 192-199.

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Immunohistochemical Localization of NR1

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Sections were immersed in 25% sucrose and 3% glycerol in 0.05 M PB (15 min) then immersed in Freon followed by liquid nitrogen (method of S. Aicher assisting G.S.Y.). Sections were treated with mouse anti-NR1 antisera (1:10; Chemicon). NR1 was visualized by immunogold labeling using goat anti-mouse IgG conjugated to 1 nm colloidal gold (1:50, Amersham). Incubations contained 0.1% BSA at 22° C (1 h) followed by 4° C (4 h). Sections were rinsed in citrate buffer. Colloidal gold was enhanced by silver intensification (IntenSEM, Amersham, Piscataway, NJ). Sections were fixed in 2% osmium tetroxide in 0.1 M PB (1 h), washed (0.1 M PB, 10 min), dehydrated using an ethanol series followed by propylene oxide and propylene oxide:EMBed (Electronmicroscopy Sciences, Fort Washington, PA; 1:1) solution, incubated in EMBed (2 h), embedded between Aclar sheets (60° C, 1–2 d), and glued to plastic blocks; 75 nm sections were collected onto copper grids and counterstained with uranyl acetate and Reynolds lead citrate. Images from the medial accessory olive were obtained using a Philips 201 electronmicroscope, recorded to 3.75×3.25 in film, and digitized (2400 pixels/in).

Turecek J., Yuen G.S., Han V.Z., Zeng X.H., Bayer K.U, & Welsh J.P. (2014). NMDA RECEPTOR ACTIVATION STRENGTHENS WEAK ELECTRICAL COUPLING IN MAMMALIAN BRAIN. Neuron, 81(6), 1375-1388.

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Immunohistochemical and Ultrastructural Analysis

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Immunohistochemistry was carried out on 10 μm sections as described in detail elsewhere (14 (link), 15 (link)). Briefly, non-specific binding sites were quenched with 5% bovine serum albumin; sections were then incubated with rabbit anti-entactin antibody (Abcam, Cambridge, UK) followed by Cy5-conjugated anti-rabbit IgG (Jackson Immunoresearch, West Grove, PA) for 45 minutes. Sections were counterstained with TRITC-conjugated phalloidin (Sigma-Aldrich) and analysed with a Zeiss LSM 510 confocal microscope. For TEM analysis, samples were processed according to standard procedure (16 (link)) and examined with a Philips 201 electron microscope.

Man A.L., Gicheva N., Regoli M., Rowley G., De Cunto G., Wellner N., Bassity E., Gulisano M., Bertelli E, & Nicoletti C. (2016). CX3CR1+ cell-mediated Salmonella-exclusion protects the intestinal mucosa during the initial stage of infection. Journal of immunology (Baltimore, Md. : 1950), 198(1), 335-343.

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Ultrastructural Analysis of ARPV Entry

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Infected C6/36 monolayers were fixed in 2.5% formaldehyde/0.1% glutaraldehyde in 0.05 M cacodylate buffer pH 7.3 containing 0.03% trinitrophenol and 0.03% CaCl₂ for at least 1 hour at room temperature. Monolayers were then washed in 0.1 M cacodylate buffer, scraped and pelleted by centrifugation. Pellets were post-fixed in 1% OsO₄, 0.1 M cacodylate buffer, pH 7.3, washed with distilled water, en bloc stained with 2% aqueous uranyl acetate for 20 minutes at 60°C, then dehydrated in graded series of ethanol and embedded in Poly/Bed 812 (Polysciences, Warrington, PA). Ultrathin sections were cut on Leica EM UC7 ultramicrotome (Leica Microsystems, Buffalo Grove, IL), stained with lead citrate, and examined in a Philips (Eindhoven, Netherlands) 201 electron microscope at 60 KV.
To visualize ARPV entry into vertebrate cells, Vero cells were infected (MOI 10 genome copies/cell) with ARPV and incubated on ice for 1 hour. Cells were removed from the ice and incubated at 37°C for 15 seconds, 1 minute, or 5 minutes before being fixed in 2.5% formaldehyde/0.1% glutaraldehyde in 0.05 M cacodylate buffer pH 7.3 containing 0.03% trinitrophenol and 0.03% CaCl₂ for 1 hour at room temperature. Monolayers were processed for microscopy similarly to C6/36 monolayers as above.

Auguste A.J., Langsjoen R.M., Porier D.L., Erasmus J.H., Bergren N.A., Bolling B.G., Luo H., Singh A., Guzman H., Popov V.L., Travassos da Rosa A.P., Wang T., Kang L., Allen I.C., Carrington C.V., Tesh R.B, & Weaver S.C. (2021). Isolation of a novel insect-specific flavivirus with immunomodulatory effects in vertebrate systems.. Virology, 562, 50-62.

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