Scepter counter

Manufactured by Merck Group

Sourced in United States

The Scepter counter is a compact, handheld instrument used for automated cell counting and sizing. It provides accurate and reliable measurements of cell concentration and size distribution in a variety of cell samples.

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Lab products found in correlation

Countbright absolute counting beads, by Thermo Fisher Scientific (1 mentions) Neural tissue dissociation kit t, by Miltenyi Biotec (1 mentions) Dnase 1, by Roche (1 mentions) Collagenase d, by Roche (1 mentions) Guava easycyte ht, by Merck Group (1 mentions) Fetal calf serum (fcs), by Euroclone (1 mentions) Rpmi 1640 medium, by Merck Group (1 mentions) Cytation 3 cell imaging reader, by Agilent Technologies (1 mentions)

Close spelling variants of this product

Scepter™ 2.0 Cell Counter (62 citations) Scepter cell counter (52 citations) Scepter handheld automated cell counter (45 citations) Scepter 2.0 Handheld Automated Cell Counter (29 citations) Scepter automated cell counter (14 citations) Scepter 2.0 automated cell counter (11 citations) Scepter handheld cell counter (5 citations) Scepter handheld automatic cell counter (3 citations) Scepter 2.0 handheld cell counter (2 citations) Scepter 3.0 handheld automated cell counter (2 citations)

5 protocols using scepter counter

Isolation and Purification of Immune Cells

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Mice were sacrificed by injecting 100 µl pentobarbital (i.p.). When applicable, blood was collected from the right ventricle prior to perfusion. Mice were perfused with ice- cold PBS and organs dissected. CNS cells were prepared by enzymatic digestion using Collagenase D (1 mg/ml, Roche) and DNAse I (0.2 mg/ml, Roche) or Neural Tissue Dissociation Kit T (Miltenyi Biotec). Myelin was removed using 38% Percoll.
Samples of 100–200 μl blood were collected into tubes containing EDTA, lysed in ACK buffer and centrifuged. The pellet was resuspended in PBS and used for staining. Spleen cell suspensions were prepared by mechanical dissociation in PBS using 40 μm strainers. BM cells were prepared by flushing femurs with PBS. Spleen and BM preparations were treated with ACK buffer to lyse RBCs. Cell suspensions from the lamina propria of the small intestine were prepared by mechanical dissociation followed by enzymatic digestion using DNAse and Liberase. Cells were then purified using a 40/60% Percoll gradient and isolated at the interphase. Cells were counted using a Scepter counter (Millipore) or by flow cytometry using CountBright absolute counting beads (Thermo Fisher).

Lund H., Pieber M., Parsa R., Han J., Grommisch D., Ewing E., Kular L., Needhamsen M., Espinosa A., Nilsson E., Överby A.K., Butovsky O., Jagodic M., Zhang X.M, & Harris R.A. (2018). Competitive repopulation of an empty microglial niche yields functionally distinct subsets of microglia-like cells. Nature Communications, 9, 4845.

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Flow Cytometric Characterization of iPSCs

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Example 18

Flow cytometry was conducted using a Guava EasyCyte HT. Cells were dissociated using Trypsin Like Enzyme (Tryp-LE) for 10 minutes at 37° C. Dissociated cells were pipetted to remove aggregations and clumps and passed through a 70-micron filter. Single cell suspensions were counted using a Millipore Scepter counter and cell density was adjusted to 1×10⁵cells/100 microliters. 5 microliters of appropriate antibody was added to the dissociated cells and mixed using gentle pipetting. This was then incubated in the dark for 30 minutes on ice. At the end of this incubation period, labeled cells were washed with 1× ice cold DPBS and resuspended in 200 microliters DPBS. Cells were then counted using a Guava EasyCyte HT. Viable cells were gated using a log/log Forward Scatter/Side Scatter plot. Each IPSC marker fluorescence was also compared to its IgKappa Isotype control to quantify non-specific and autofluorescence events. Each IPSC marker was counted and plotted as a graph with the abscissa containing the log Fluorescence of a given marker and the ordinate containing the counts of either a negative or positive viable gated cell. This graph was then used to create histograms providing percentages of negative and positive cells. Based on the IgKappa Isotype control, 10²was used as the cutoff in log Fluorescence between a negative and a positive cell.

US11268070B2. Methods for creating integration-free, virus-free, exogenous oncogene-free IPS cells and compositions for use in such methods (2022-03-08). Cellular Engineering Technologies, Inc. [US]. Inventors: Alan B. Moy [US], Anant Kamath [US].

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Proliferation and Cytotoxicity Assays

Cited 1 time

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For proliferation kinetics, cells were seeded in 6-well plates. Every 24 hours, duplicate wells were counted using a Scepter Counter (Millipore). For cytotoxicity assays, cells were seeded in a 96 well plate. After 24 hours, cells were treated with serial dilutions of the indicated drug, with a fixed concentration of 1 µM NVP-AEW541 or 10 µg/ml IMC-A12 if indicated. After 72 hours of treatment, the relative cell number in each well was determined using the Sulforhodamine B assay [7] (link). The IC₅₀ is the drug concentration corresponding to a 50% decrease in cell number calculated using the dose-effect curve.

Brouwer-Visser J., Lee J., McCullagh K., Cossio M.J., Wang Y, & Huang G.S. (2014). Insulin-Like Growth Factor 2 Silencing Restores Taxol Sensitivity in Drug Resistant Ovarian Cancer. PLoS ONE, 9(6), e100165.

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IFN-γ Production in BSL-4 Leukocytes

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IFN-γ production was evaluated in BSL-4 laboratory after mitogenic stimulation. Specifically, leukocytes from patients in viremic and non-viremic phases were thawed, counted by Scepter counter (Millipore) and seeded at 3x10⁵cells/well in RPMI-1640 medium (Sigma-Aldrich, St. Louis, MS, USA) supplemented with 10% pre-tested heat inactivated FCS (Euroclone, Italy). Cells were then stimulated with phytohemagglutinin (PHA, 5 mg/ml) for 20 h, and the immunological competence was evaluated by IFN-γ enzyme-linked immunospot assay (ELISpot assay, AID Diagnostika, Germany). Leukocytes from healthy donors were used as internal positive controls. CD3 T-cells count from the same samples was evaluated by flow cytometry as described above, and the results of the ELISpot assay were then normalized as spot forming cells (SFC)/10⁵ CD3.

Agrati C., Castilletti C., Casetti R., Sacchi A., Falasca L., Turchi F., Tumino N., Bordoni V., Cimini E., Viola D., Lalle E., Bordi L., Lanini S., Martini F., Nicastri E., Petrosillo N., Puro V., Piacentini M., Di Caro A., Kobinger G.P., Zumla A., Ippolito G, & Capobianchi M.R. (2016). Longitudinal characterization of dysfunctional T cell-activation during human acute Ebola infection. Cell Death & Disease, 7(3), e2164-.

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Quantitative Cell Imaging of CDs

Cited 1 time

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Cells were plated using Scepter counter^® (Millipore, USA) at 2500 cells/well in 96 well plate. After 48 h, 100 µL of culture medium containing CDs solutions at the concentrations used in cytotoxicity assay were added to the wells. Cells were incubated in presence of CDs for 24 h. Cell imaging was performed on Cytation 3 platform: Images were taken at × 4 to × 40 magnification using Cytation 3 cell imaging reader (Biotek Instrument Inc., Colmar, France) on living cells maintained at 37 °C. Fluorescence intensities were measured at magnification × 20 with filters 469/525 (green), 586/647 (red), 377/447 (blue), 8 wells per CD were analyzed. Image and fluorescence data were retrieved using the software Gen5 2.08 (Biotek Instruments, Winooski, USA) [16 (link)].

Kuznietsova H., Géloën A., Dziubenko N., Zaderko A., Alekseev S., Lysenko V, & Skryshevsky V. (2023). In vitro and in vivo toxicity of carbon dots with different chemical compositions. Discover Nano, 18(1), 111.

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