Glow discharged copper grid

Manufactured by Ted Pella

The Glow-discharged copper grid is a laboratory equipment item used for sample preparation in electron microscopy. It provides a conductive surface to support and stabilize samples during imaging. The glow-discharge process creates a hydrophilic surface on the copper grid, enhancing the wetting and adhesion of the sample material.

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Copper grids (53 citations) Glow-discharged carbon coated copper grid (2 citations) Glow-discharged formvar/carbon-coated 400 mesh copper grid (2 citations) Glow discharged 300 mesh copper grids (2 citations) Glow discharged formvar/carbon 200 mesh copper grids (2 citations)

3 protocols using glow discharged copper grid

Transmission Electron Microscopy Imaging of Engineered Nanoparticles

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2 μl of eNP sample at a concentration of 1 mg/ml in PBS was applied to a glow-discharged copper grid (Ted Pella), washed twice with buffer before staining with 2% uranyl acetate for 30 s and air dried. Grids were imaged using a JEOL JEM-1400plus Transmission Electron Microscope operating at 80 kV and recorded with an AMT XR111 high-speed 4k × 2k pixel phosphor-scintillated 12-bit CCD camera.

Su Z., Wu C., Shi L., Luthra P., Pintilie G.D., Johnson B., Porter J.R., Ge P., Chen M., Liu G., Frederick T.E., Binning J.M., Bowman G.R., Zhou Z.H., Basler C.F., Gross M.L., Leung D.W., Chiu W, & Amarasinghe G.K. (2018). Electron cryo-microscopy structure of Ebola nucleoprotein reveals a mechanism for nucleocapsid-like assembly. Cell, 172(5), 966-978.e12.

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Transmission Electron Microscopy of Samples

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Two microliter of N sample at a concentration of 1 mg/mL was applied to a glow-discharged copper grid (Ted Pella), washed twice with water before staining with 2% uranyl acetate for 30 s, and air-dried. Grids were imaged using a JEOL JEM-1400Plus Transmission Electron Microscope operating at 120 kV and recorded with an AMT XR111 high-speed 4k x 2k pixel phosphor-scintillated 12-bit CCD camera.

Wu C., Qavi A.J., Hachim A., Kavian N., Cole A.R., Moyle A.B., Wagner N.D., Sweeney-Gibbons J., Rohrs H.W., Gross M.L., Peiris J.S., Basler C.F., Farnsworth C.W., Valkenburg S.A., Amarasinghe G.K, & Leung D.W. (2021). Characterization of SARS-CoV-2 nucleocapsid protein reveals multiple functional consequences of the C-terminal domain. iScience, 24(6), 102681.

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Cryo-EM Imaging of N Samples

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2 μL of N sample at a concentration of 1 mg/mL was applied to a glow-discharged copper grid (Ted Pella), washed twice with water before staining with 2% uranyl acetate for 30 s and air dried. Grids were imaged using a JEOL JEM-1400plus Transmission Electron Microscope operating at 120 kV and recorded with an AMT XR111 high-speed 4k × 2k pixel phosphor-scintillated 12-bit CCD camera.

Wu C., Qavi A.J., Hachim A., Kavian N., Cole A.R., Moyle A.B., Wagner N.D., Sweeney-Gibbons J., Rohrs H.W., Gross M.L., Peiris J.S., Basler C.F., Farnsworth C.W., Valkenburg S.A., Amarasinghe G.K, & Leung D.W. (2020). Characterization of SARS-CoV-2 N protein reveals multiple functional consequences of the C-terminal domain. bioRxiv.

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