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Ec plan neofluar x10 air objective na 0.3 wd 5 2mm

Manufactured by Zeiss
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The EC Plan-Neofluar X10 air objective is a microscope objective lens with a 10x magnification, numerical aperture (NA) of 0.3, and a working distance (WD) of 5.2 mm. It is designed for use in air and provides high-quality imaging performance.

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Lab products found in correlation

W plan apochromat x20 water objective na 1.0 wd 1 17mm, by Zeiss (2 mentions) Opera phenix microscope, by PerkinElmer (2 mentions) Sensoplate, by Greiner (2 mentions)

2 protocols using ec plan neofluar x10 air objective na 0.3 wd 5 2mm

1

Time-lapse Imaging of 3D Co-cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
CBF-0.5 hydrogels containing mPCOs, PaFs and BMDMs 1:5:5 ratio of PDO:Paf:BMDM were prepared as described previously and crafted into 10 μL domes in 24-well Sensoplate (Greiner). Co-culture domes were imaged via the PerkinElmer Opera Phenix microscope. A Zeiss EC Plan Neofluar X10 air objective NA 0.3 WD 5.2mm or Zeiss W Plan-Apochromat X20 water objective NA 1.0 WD 1.17mm were used as indicated. Pre-scan directed z-stack acquisition of individual spheres within the gel was used and co-cultures were imaged every hour over a time-window of 72h starting from day three of culture. Videos and representative still images were generated from maximum intensity projections using Harmony 4.8 software. Representative images were exported from Harmony 4.8 in the portable network graphic format with scaling equally applied to all conditions.
Below C.R., Kelly J., Brown A., Humphries J.D., Hutton C., Xu J., Lee B.Y., Cintas C., Zhang X., Hernandez-Gordillo V., Stockdale L., Goldsworthy M.A., Geraghty J., Foster L., O’Reilly D.A., Schedding B., Askari J., Burns J., Hodson N., Smith D.L., Lally C., Ashton G., Knight D., Mironov A., Banyard A., Eble J.A., Morton J.P., Humphries M.J., Griffith L.G, & Jørgensen C. (2021). A microenvironment-inspired synthetic 3D model for pancreatic ductal adenocarcinoma organoids. Nature materials, 21(1), 110-119.
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2

Time-lapse Imaging of 3D Co-cultures

Check if the same lab product or an alternative is used in the 5 most similar protocols
CBF-0.5 hydrogels containing mPCOs, PaFs and BMDMs 1:5:5 ratio of PDO:Paf:BMDM were prepared as described previously and crafted into 10 μL domes in 24-well Sensoplate (Greiner). Co-culture domes were imaged via the PerkinElmer Opera Phenix microscope. A Zeiss EC Plan Neofluar X10 air objective NA 0.3 WD 5.2mm or Zeiss W Plan-Apochromat X20 water objective NA 1.0 WD 1.17mm were used as indicated. Pre-scan directed z-stack acquisition of individual spheres within the gel was used and co-cultures were imaged every hour over a time-window of 72h starting from day three of culture. Videos and representative still images were generated from maximum intensity projections using Harmony 4.8 software. Representative images were exported from Harmony 4.8 in the portable network graphic format with scaling equally applied to all conditions.
Below C.R., Kelly J., Brown A., Humphries J.D., Hutton C., Xu J., Lee B.Y., Cintas C., Zhang X., Hernandez-Gordillo V., Stockdale L., Goldsworthy M.A., Geraghty J., Foster L., O’Reilly D.A., Schedding B., Askari J., Burns J., Hodson N., Smith D.L., Lally C., Ashton G., Knight D., Mironov A., Banyard A., Eble J.A., Morton J.P., Humphries M.J., Griffith L.G, & Jørgensen C. (2021). A microenvironment-inspired synthetic 3D model for pancreatic ductal adenocarcinoma organoids. Nature materials, 21(1), 110-119.
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