Csb e07282r

The concentrations of estradiol and progesterone in the plasma of wild ground squirrels were measured using ELISA kits (CSB-E05110r for estradiol, CSB-E07282r for progesterone, CSB-E07271r for adiponectin, Cusabio Biotech Co., Ltd., Wuhan, China) according to the protocol. The data at 450 nm were subsequently read with a microplate reader (PT 3502 g, Beijing Potenov Technology Co., Ltd., Beijing, China). The intra-assay and inter-assay coefficient of variation for estradiol were 5.1% and 8.9%, respectively. In the hormone determination of progesterone, the intra- and inter-assay co-efficient of variation were 3.9% and 6.7%, respectively. The intra-assay co-efficient of variation was 4.5% and the inter-assay co-efficient of variation was 7.3% in the hormone determination of adiponectin.

Enzyme-linked immunosorbent assay (ELISA) kits were used to measure the serum concentrations of HSP70 (DYC1663E, Minneapolis, MN, USA), follicle stimulating hormone (FSH)(CSB-E06869r, Cusabio Biotech CO.,Ltd., Wuhan, China), luteinizing hormone (LH) (CSB-E12654r, Cusabio Biotech CO.,Ltd., Wuhan, China), estradiol (E2) (CSB-E05110r, Cusabio Biotech CO.,Ltd., Wuhan, China), progesterone (P) (CSB-E07282r, Cusabio Biotech CO.,Ltd., Wuhan, China), testosterone (T)(E-EL-0072c, Elabscience Biotechnology Co.,Ltd., Wuhan, China), insulin (CSB-E05070r, Elabscience Biotechnology Co.,Ltd., Wuhan, China), C-reactive protein (CRP) (E-EL-R0022c, Elabscience Biotechnology Co.,Ltd., Wuhan, China), interleukin (IL)-6(E-EL-R0015c, Elabscience Biotechnology Co.,Ltd., Wuhan, China), IL-18(E-EL-R0567c, Elabscience Biotechnology Co.,Ltd., Wuhan, China), tumor necrosis factor (TNF)-α. (E-EL-R0019c, Elabscience Biotechnology Co.,Ltd., Wuhan, China). There were standard curves used to calculate for all these kits. The coefficients of variation within and between plates were less than 10%.