C57BL/6 mice were purchased from Chubu Kagaku Shizai (Nagoya, Japan). All the mice were handled in accordance with the Guidelines for Animal Experiments, Gifu University (Gifu, Japan). Mice are sacrificed by cervical dislocation. The APC-Cy7-conjugated CD4, PE-Cy-7-conjugated CD8 antibodies, PE-conjugated CD80 antibody, the purified anti-mouse CD16/32, the APC-Cy7-conjugated rat anti-mouse CD45, APC-Cy7-conjugated TER-119, and PE-conjugated anti-mouse EpCAM (G8.8) were obtained from Biolegend (San Diego, CA). The rabbit anti-mouse keratin-5 antibody was purchased from Covance (Berkeley, CA). The biotinylated anti-mouse keratin-8 antibody was from PROGEN (Heidelberg, Germany). The biotinylated UEA-1 was obtained from VECTOR Laboratories (Burlingame, CA). Further, 7-aminoactinomycin D was purchased from Wako (Tokyo, Japan).
7 aminoactinomycin d
Manufactured by Fujifilm
Sourced in Japan
7-aminoactinomycin D is a fluorescent dye used in flow cytometry and microscopy applications. It is a nucleic acid stain that binds to DNA, emitting fluorescence when excited by a specific wavelength of light. This dye is commonly used for cell viability and cell cycle analysis.
Automatically generated - may contain errors
Lab products found in correlation
Biotinylated uea 1, by Vector Laboratories (2 mentions)
Purified anti mouse cd16 32, by BioLegend (1 mentions)
Apc cy7 conjugated cd4, by BioLegend (1 mentions)
Fitc conjugated cd3e, by BD (1 mentions)
Alexa fluor 546 conjugated streptavidin, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 conjugated anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Apc cy7 conjugated rat anti mouse cd45, by BD (1 mentions)
Anti fcγ 3 2 receptor 2.4g2, by BD (1 mentions)
Dnase 1, by Merck Group (1 mentions)
Canto 2, by BD (1 mentions)
Liberase, by Roche (1 mentions)
3 protocols using 7 aminoactinomycin d
1
Murine Immune Cell Isolation and Characterization
2
Multiparametric Flow Cytometry Analysis of Immune Cell Populations
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rabbit anti–mouse keratin-5 antibody was purchased from Covance. Biotinylated UEA-1 was obtained from Vector Laboratories. The APC-Cy7–conjugated rat anti–mouse CD45, PE-Cy7–conjugated TER119, and PE-conjugated anti–mouse EpCAM (G8.8) antibodies were obtained from BD or BioLegend. The PE-conjugated rat anti-CD45, PE-conjugated mouse TER-119, FITC-conjugated mouse MHC II (M5/114.15.1), and APC-conjugated Aire antibodies were obtained from eBioscience. PE-conjugated CD80, CD86, CD40, and PD-L1 antibodies, purified anti–mouse CD16/32, FITC-conjugated ant–mouse EpCAM (G8.8), APC-Cy7–conjugated CD4, PE–Cy-7–conjugated CD8, and APC-conjugated GITR antibodies were obtained from BioLegend. PE-conjugated CD8, FITC-conjugated CD4, and FITC-conjugated CD3e antibodies were obtained from BD. PE-conjugated anti-Foxp3 (FJK-16s) was purchased from eBioscience. 7-aminoactinomycin D and recombinant mouse RANKL were purchased from Wako Pure Chemical Industries. Anti–mouse OPG antibody was purchased from R&D Systems. A RANK-Fc chimera was obtained from Sigma-Aldrich. Alexa Fluor 546–conjugated streptavidin and Alexa Fluor 488–conjugated anti–rabbit IgG were obtained from Invitrogen.
3
Thymic Cell Isolation and Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total thymic cells were prepared by digestion in RPMI 1640 medium containing Liberase (Roche) and DNase I (Sigma). For thymocytes analysis, single cell suspensions from the thymus were prepared mechanically. Before staining, cells were preincubated with anti-Fcγ III/II receptor (2.4G2) (BD Pharmingen) to block Fc receptors. Cells were stained with antibodies in PBS containing 2% FBS. Dead cells were excluded from the analysis by 7-aminoactinomycin D (Wako) staining. Stained cells were analyzed with a fluorescence-activated cell sorter (FACS; Canto II; BD Bioscience).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!