Phospho tau

Manufactured by Abcam

Phospho-tau is a laboratory product used for detecting and measuring phosphorylated tau protein, a key component associated with neurodegenerative diseases. The product provides a tool for researchers to investigate the role of tau phosphorylation in disease processes.

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Lab products found in correlation

P44 42 mapk erk1 2, by Cell Signaling Technology (1 mentions) Hrp conjugated affinipure goat anti rabbit igg, by Proteintech (1 mentions) Phospho p44 42 mapk, by Cell Signaling Technology (1 mentions) Phospho mek1 2, by Cell Signaling Technology (1 mentions) Propidium iodide, by Absin (1 mentions) Oil red o, by Merck Group (1 mentions) Cavin1, by Proteintech (1 mentions) Hrp conjugated affinipure goat anti mouse igg, by Proteintech (1 mentions) Dhcr24, by Cell Signaling Technology (1 mentions) Filipin 3, by Absin (1 mentions) Phospho tau ser396, by Cell Signaling Technology (1 mentions) Ras 27h5, by Cell Signaling Technology (1 mentions) U0126, by Cell Signaling Technology (1 mentions) Chemidoc xr, by Bio-Rad (1 mentions) Molecular imager, by Bio-Rad (1 mentions) Electrotransfer apparatus, by Bio-Rad (1 mentions) Gsk 3, by Abcam (1 mentions) Psd 95, by Thermo Fisher Scientific (1 mentions) Hsp70, by Enzo Life Sciences (1 mentions) Mmp 9, by Abcam (1 mentions)

Spelling variants of this product

Recombinant anti-tau (phospho T231) antibody (2 citations) Phospho-tau ser396 (2 citations) Anti-Tau (phospho T181; (2 citations)

3 protocols using phospho tau

Tau Phosphorylation and Signaling Assays

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DHCR24 (#2033, Cell Signaling Technology), Tau5 (AHB0042, Thermo), Phospho-Tau (Thr181) (ab254409, Abcam), Phospho-Tau (Ser199) (#29,957, Cell Signaling Technology), Phospho-Tau (Ser396) (#9632, Cell Signaling Technology), Actin (#2118, Proteintech), cavin1 (#18,892–1-AP, Proteintech), Phospho-Tau (Ser262)(ab131354, Abcam), Phospho-Tau (Thr231)(#4137, ABclonal), Ras(27H5)(#3339, Cell Signaling Technology), p44/42 MAPK (ERK1/2) (137F5)(#4695S, Cell Signaling Technology), Phospho-p44/42 MAPK (ERK1/2)(Thr202/Tyr204)(#4730S, Cell Signaling Technology), MEK1/2 (D1A5)(#8727, Cell Signaling Technology), Phospho-MEK1/2 (Ser217/221)(#9154, Cell Signaling Technology), U0126 (#9903, Cell Signaling Technology), Propidium Iodide (abs9105, Absin), Methyl-β-cyclodextrin (abs42021762, Absin), Filipin III (abs42018484, Absin), Oil Red O (CAS:1320–06-5, O0625-25G, Sigma), HRP-conjugated Affinipure Goat Anti-Rabbit IgG(H + L)(SA00001-2, Proteintech), HRP-conjugated Affinipure Goat Anti-Mouse IgG (H + L)(SA00001-1, Proteintech).

Mai M., Guo X., Huang Y., Zhang W., Xu Y., Zhang Y., Bai X., Wu J, & Zu H. (2022). DHCR24 Knockdown Induces Tau Hyperphosphorylation at Thr181, Ser199, Ser262, and Ser396 Sites via Activation of the Lipid Raft-Dependent Ras/MEK/ERK Signaling Pathway in C8D1A Astrocytes. Molecular Neurobiology, 59(9), 5856-5873.

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Western Blot Analysis of Neuronal Markers

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Equal quantities of mice brain extracts (25μg) were separated on polyacrylamide gel under reducing condition and transferred to polyvinyldifluoride membrane using an electrotransfer apparatus (Bio-Rad, Hercules, CA, USA). After blocking with 5 % non-fat dry milk for 1h, the membranes were probed with a primary antibody overnight at 4°C (PrP^c, Sigma, 1:500; Glycogen synthase kinase-3 (GSK-3), Abcam, 1: 500; Microtubule-associated protein-2 (MAP-2), Abcam, 1:500; tau, Abcam, 1:1000; and, phospho-tau, Abcam, 1:1000). Next day, the membranes were probed with the appropriate secondary antibody (Santa cruz, 1:5000) for 1 h at room temperature and then developed using a Bio-Rad Molecular Imager (ChemiDoc XRS+, Hercules, CA, USA).

Kalani A., Chaturvedi P., Maldonado C., Bauer P., Joshua I.G., Tyagi S.C, & Tyagi N. (2017). Dementia-like pathology in type-2 diabetes: A novel microRNA mechanism. Molecular and cellular neurosciences, 80, 58-65.

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Protein Profiling of Rat Hippocampal Samples

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Rat brain hippocampal samples were lysed in a lysis buffer containing a protease inhibitor (11697498001, Sigma, USA), a phosphatase inhibitor (4906845001, Sigma, USA), and an RIPA buffer (IBS-BR002, iNtRON, Republic of Korea), and then centrifuged for 20 min at 14,000 rpm, at 4°C. Supernatant protein quantification was carried out using a Bradford protein assay. Quantified supernatant was boiled in an SDS loading buffer. Separately divided proteins were transferred to a PVDF membrane after SDS-PAGE. Primary antibodies to the following target antigens were used: phospho-AMPK (Abcam, 1:1 k), AMPKα (Cell signaling, 1:1 k), MMP-9 (Abcam, 1:1 k), β-actin (Cell signaling, 1:10 k), HSP-70 (Enzo, 1:1 k), phospho-Tau (Abcam, 1:50 k), and PSD-95 (Invitrogen, 1:1 k).

Hong D.K., Eom J.W., Kho A.R., Lee S.H., Kang B.S., Lee S.H., Koh J.Y., Kim Y.H., Choi B.Y, & Suh S.W. (2022). The Inhibition of Zinc Excitotoxicity and AMPK Phosphorylation by a Novel Zinc Chelator, 2G11, Ameliorates Neuronal Death Induced by Global Cerebral Ischemia. Antioxidants, 11(11), 2192.

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